Improving the cellobiose hydrolysis activity of glucose-stimulating β-glucosidase Bgl2A

Liu, Shuaifeng; Zhang, Meng; Hong, Daqing; Fang, Zemin; Xiao, Yazhong; Fang, Wei; Zhang, Xuecheng

doi:10.1016/j.enzmictec.2023.110289

Cited by 3 publications

(2 citation statements)

References 52 publications

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“…However, intensifying sulfuric acid pretreatment to enhance hydrolysis exacerbates inhibitor production, contrary to our objectives. Previous studies have shown that β -glucosidase effectively alleviates cellobiose feedback inhibition, thereby enhancing enzymatic hydrolysis efficiency [ 60 ]. Through the addition of 10 U/g β -glucosidase, enzymatic hydrolysis efficiency increased to 91.36% ( Table 6 ).…”

Section: Resultsmentioning

confidence: 99%

Highly Efficient Production of Cellulosic Ethanol from Poplar Using an Optimal C6/C5 Co-Fermentation Strain of Saccharomyces cerevisiae

Xu,

Sun,

Wang

et al. 2024

Microorganisms

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Cellulosic ethanol is the key technology to alleviate the pressure of energy supply and climate change. However, the ethanol production process, which is close to industrial production and has a high saccharification rate and ethanol yield, still needs to be developed. This study demonstrates the effective conversion of poplar wood waste into fuel-grade ethanol. By employing a two-step pretreatment using sodium chlorite (SC)-dilute sulfuric acid (DSA), the raw material achieved a sugar conversion rate exceeding 85% of the theoretical value. Under optimized conditions, brewing yeast co-utilizing C6/C5 enabled a yield of 35 g/L ethanol from 10% solid loading delignified poplar hydrolysate. We increased the solid loading to enhance the final ethanol concentration and optimized both the hydrolysis and fermentation stages. With 20% solid loading delignified poplar hydrolysate, the final ethanol concentration reached 60 g/L, a 71.4% increase from the 10% solid loading. Our work incorporates the pretreatment, enzymatic hydrolysis, and fermentation stages to establish a simple, crude poplar waste fuel ethanol process, expanding the range of feedstocks for second-generation fuel ethanol production.

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Section: Resultsmentioning

confidence: 99%

Highly Efficient Production of Cellulosic Ethanol from Poplar Using an Optimal C6/C5 Co-Fermentation Strain of Saccharomyces cerevisiae

Xu,

Sun,

Wang

et al. 2024

Microorganisms

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“…To address such issues, one could adjust the enzyme formulation to improve the efficiency of enzymatic hydrolysis. For example, adding an additive enzyme ( β -glucosidase) to mitigate the negative feedback inhibition from its own produced final products or intermediates is one of the interesting approaches (for example, cellobiose, an intermediate that strongly inhibits the activity of cellulase) [ 28 , 29 ]. Therefore, strategies involving synergistic roles of enzymes, enzyme loading, and supplementation of β -glucosidase are continuously accessed for futuristic advancement in the enzymatic hydrolysis of cellulosic biomass.…”

Section: Methods Of Cellulosic Biomass Conversionmentioning

confidence: 99%

Light-Driven Depolymerization of Cellulosic Biomass into Hydrocarbons

Negi,

Kesari

2023

Polymers

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Cellulose and hemicellulose are the main constituents of lignocellulosic biomass. Chemical derivatization of lignocellulosic biomass leads to a range of C5 and C6 organic compounds. These C5 and C6 compounds are valuable precursors (or fine chemicals) for developing sustainable chemical processes. Therefore, depolymerization of cellulose and hemicellulose is essential, leading to the development of various materials that have applications in biomaterial industries. However, most depolymerized processes for cellulose have limited success because of its structural quality: crystallinity, high hydrogen-bond networking, and mild solubility in organic and water. As a result, various chemical treatments, acidic (mineral or solid acids) and photocatalysis, have developed. One of the significant shortcomings of acidic treatment is that the requirement for high temperatures increases the commercial end cost (energy) and hampers product selectivity. For example, a catalyst with prolonged exposure to high temperatures damages the catalyst surface over time; therefore, it cannot be used for iterative cycles. Photocatalysts provide ample application to overcome such flaws as they do not require high temperatures to perform efficient catalysis. Various photocatalysts have shown efficient cellulosic biomass conversion into its C6 and C5 hydrocarbons and the production of hydrogen (as a green energy component). For example, TiO2-based photocatalysts are the most studied for biomass valorization. Herein, we discussed the feasibility of a photocatalyst with application to cellulosic biomass hydrolysis.

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Enhancing biogas production with fungi from oil palm empty fruit bunches: Isolation and innovative application

Ali,

Senda,

Yanti

et al. 2024

Case Studies in Chemical and Environmental Engineering

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Improving the cellobiose hydrolysis activity of glucose-stimulating β-glucosidase Bgl2A

Cited by 3 publications

References 52 publications

Highly Efficient Production of Cellulosic Ethanol from Poplar Using an Optimal C6/C5 Co-Fermentation Strain of Saccharomyces cerevisiae

Highly Efficient Production of Cellulosic Ethanol from Poplar Using an Optimal C6/C5 Co-Fermentation Strain of Saccharomyces cerevisiae

Light-Driven Depolymerization of Cellulosic Biomass into Hydrocarbons

Enhancing biogas production with fungi from oil palm empty fruit bunches: Isolation and innovative application

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