Improving the activity and thermal stability of trypsin by the rational design

Xiao, Tianxiang; Li, Zhouquan; Xing, Xingyue; He, Feifan; Jie, Huang; Xue, Dongsheng

doi:10.1016/j.procbio.2023.04.024

Cited by 4 publications

(1 citation statement)

References 42 publications

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“…Trypsin is known to hydrolyze proteins after positively charged lysine (K) and arginine (R) residues. In contrast, chymotrypsin hydrolyzes proteins preferentially after aromatic amino acids (tryptophan (W), phenylalanine (F), tyrosine (Y)) and leucine (L) [ 32 , 33 , 34 ].…”

Section: Resultsmentioning

confidence: 99%

Identification of Antibody-Mediated Hydrolysis Sites of Oligopeptides Corresponding to the SARS-CoV-2 S-Protein by MALDI-TOF Mass Spectrometry

Timofeeva,

Sedykh,

Dmitrenok

et al. 2023

IJMS

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Antibodies recognizing RBD and the S-protein have been previously demonstrated to be formed in humans after SARS-CoV-2 infection and vaccination with the Sputnik V adenovirus vaccine. These antibodies were found to be active when hydrolyzing FITC-labeled oligopeptides corresponding to linear epitopes of the S-protein. The thin-layer chromatography method allows the relative accumulation of the reaction product to be estimated but cannot identify hydrolysis sites. This study used the MALDI-TOF MS method to establish oligopeptide hydrolysis sites. Using the MALDI-TOF MS method in combination with the analysis of known hydrolysis sites characteristic of canonical proteases allowed us to establish the unique hydrolysis sites inherent only to catalytically active antibodies. We have discovered two 12-mer oligopeptides to have six hydrolysis sites equally distributed throughout the oligopeptide. The other three oligopeptides were found to have two to three closely spaced hydrolysis sites. In contrast to trypsin and chymotrypsin proteases, the catalytically active antibodies of COVID-19 patients have their peptide bond hydrolyzed mainly after proline, threonine, glycine, or serine residues. Here, we propose a new high-throughput experimental method for analyzing the proteolytic activity of natural antibodies produced in viral pathology.

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Section: Resultsmentioning

confidence: 99%