Improving the accuracy and precision of an arsenic field test kit: increased reaction time and digital image analysis

Kearns, James K.; Tyson, Julian F.

doi:10.1039/c2ay05655k

Cited by 23 publications

(21 citation statements)

References 23 publications

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“…[2][3][4][5][6][7][8][9][10][11][12][13][14][15] Ozcan and co-workers and Henry and co-workers have recently reviewed the literature in the field. [17][18][19] Test strips, however, typically only provide semi-quantitative results; for full quantitation, test kits for liquid samples remain the gold standard.…”

Section: Introductionmentioning

confidence: 99%

Broadly Available Imaging Devices Enable High-Quality Low-Cost Photometry

et al. 2015

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This paper demonstrates that, for applications in resource-limited environments, expensive microplate spectrophotometers-which are used in many central laboratories for parallel measurement of absorbance of samples-can be replaced by photometers based on inexpensive and ubiquitous, consumer electronic devices (e.g., scanners and cell-phone cameras). Two devices-i) a flatbed scanner operating in transmittance mode and ii) a camera-based photometer constructed from a cell phone camera, a planar light source, and a cardboard box-demonstrate the concept. These devices illuminate samples in microtiter plates from one side, and use the RGB-based imaging sensors of the scanner/camera to measure the light transmitted to the other side. The broadband absorbance of samples (RGB-resolved absorbance) can be calculated using the RGB color values of only three pixels per micro-well. Rigorous theoretical analysis establishes a well-defined relationship between the absorbance spectrum of a sample and its corresponding RGB-resolved absorbance. The linearity and precision of measurements performed with these low-cost photometers on different dyes that absorb across the range of the visible spectrum, and chromogenic products of assays (e.g., enzymatic, ELISA) demonstrates that these low-cost photometers can be used reliably in a broad range of chemical and biochemical analyses. The ability to perform accurate measurements of absorbance on liquid samples, in parallel and at low cost, would enable testing, typically reserved for well-equipped clinics and laboratories, to be performed in circumstances where resources and expertise are limited.

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Section: Introductionmentioning

confidence: 99%

Broadly Available Imaging Devices Enable High-Quality Low-Cost Photometry

et al. 2015

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“…54 In other cases, it has been further sought to improve the accuracy of the methods via even longer reaction times and imaging of the test strips using a flatbed scanner. 55 A study from Nebraska, USA looking at the Quick Arsenic and Hach EZ kits has concluded favourably with regards to their routine use. 48 Evaluating against the US Environmental Protection Agency (US EPA) standard of 10 µg/L of arsenic in solution, the authors have found that, largely, water samples containing 15 µg/L or Table 2: General comparison of the performance attributes of various portable arsenic test kits based on the Gutzeit reaction.…”

Section: Field Kit Evaluationsmentioning

confidence: 99%

Detection of trace arsenic in drinking water: challenges and opportunities for microfluidics

Yogarajah¹,

Tsai²

2023

Preprint

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Arsenic contamination of drinking water is a major global problem, with contamination in Bangladesh deemed most serious. Although the current World Health Organisation (WHO) maximum contamination limit (MCL) for arsenic in drinking water is 10 μg L−1, due to practical and economic constraints, the standard limit in Bangladesh and many other developing nations is 50 μg L−1. We propose that an ideal arsenic sensor, designed for routine monitoring, will have five essential qualities: sensitivity and selectivity for arsenic; speed and reliability; portability and robustness; reduced health and environmental risks; and affordability and ease of use for local technicians. It is our opinion that many of these characteristics can be accentuated by microfluidic systems. We describe candidate colorimetric, electrochemical, biological, electrophoretic, surface-sensing, and spectroscopic methods for arsenic detection; and comment on their potential for portable microfluidic adaptation. We also describe existing developments in the literature towards the ultimate creation of microfluidic total analysis systems (μTASs) for arsenic detection. The fundamental purpose of this review is to highlight the need for better portable arsenic contamination detection, and describe how microfluidic technology may be developed to address this need.

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“…For three summers, 30 teacher participants learned about the problems of arsenic contamination of groundwater and of rice and of the role of spectrochemical analysis (as exemplified, at least for the arsenic-related projects, by the Gutzeit-Marsh reaction augmented by digital image analysis) in supporting such investigations. This theme, of improvement through digital image analysis, formed the basis of part of a doctoral dissertation in, and subsequent publication by, the Tyson group [18]. The teacher participants took materials back with them to further develop curricular materials for their students.…”

Section: Innovative Technology Experiences For Students and Teachersmentioning

confidence: 99%

Integrating Arsenic-Related Environmental Topics into the Education of the Next Generation of Citizens for Arsenic-Hit Communities: Awareness and Mobilization

Tyson¹,

Ray

Begum

et al. 2015

IJEMA

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Improving the accuracy and precision of an arsenic field test kit: increased reaction time and digital image analysis

Cited by 23 publications

References 23 publications

Broadly Available Imaging Devices Enable High-Quality Low-Cost Photometry

Broadly Available Imaging Devices Enable High-Quality Low-Cost Photometry

Detection of trace arsenic in drinking water: challenges and opportunities for microfluidics

Integrating Arsenic-Related Environmental Topics into the Education of the Next Generation of Citizens for Arsenic-Hit Communities: Awareness and Mobilization

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