Improving Spatial Resolution of a LTQ Orbitrap MALDI Source

Montero, Raúl; Abad‐García, Beatriz; Garate, José Francisco Valencia; Martín-Saiz, Lucía; Barceló‐Coblijn, Gwendolyn; Fernández, José A.

doi:10.1021/jasms.0c00133

Cited by 5 publications

(11 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Histological sections were prepared and analyzed by MALDI-IMS as described by Garate et al Briefly, DAN was used as a matrix for negative-ion detection and deposited with the aid of our in-house designed sublimator . Sections were scanned in negative-ion mode at 10 μm/pixel for nontumoral tissue and 25 μm/pixel for ccRCC sections, using an orbitrap analyzer (MALDI-LTQ-Orbitrap XL, Thermo Fisher, San Jose, CA), equipped with a modified MALDI source …”

Section: Methodsmentioning

confidence: 99%

“…32 Sections were scanned in negative-ion mode at 10 μm/pixel for nontumoral tissue and 25 μm/pixel for ccRCC sections, using an orbitrap analyzer (MALDI-LTQ-Orbitrap XL, Thermo Fisher, San Jose, CA), equipped with a modified MALDI source. 33 Data were acquired with a mass resolution of 60 000 at m/z = 400 and a laser energy of ∼10−20 μJ/pulse, depending on the spatial resolution. MALDI-IMS experiments were completed and the matrix was removed.…”

Section: Materials Andmentioning

confidence: 99%

See 1 more Smart Citation

Using the Synergy between HPLC-MS and MALDI-MS Imaging to Explore the Lipidomics of Clear Cell Renal Cell Carcinoma

et al. 2023

Self Cite

View full text Add to dashboard Cite

Lipid imaging mass spectrometry (LIMS) has been tested in several pathological contexts, demonstrating its ability to segregate and isolate lipid signatures in complex tissues, thanks to the technique's spatial resolution. However, it cannot yet compete with the superior identification power of high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS), and therefore, very often, the latter is used to refine the assignment of the species detected by LIMS. Also, it is not clear if the differences in sensitivity and spatial resolution between the two techniques lead to a similar panel of biomarkers for a given disease. Here, we explore the capabilities of LIMS and HPLC-MS to produce a panel of lipid biomarkers to screen nephrectomy samples from 40 clear cell renal cell carcinoma patients. The same set of samples was explored by both techniques, and despite the important differences between them in terms of the number of detected and identified species (148 by LIMS and 344 by HPLC-MS in negative-ion mode) and the presence/absence of image capabilities, similar conclusions were reached: using the lipid fingerprint, it is possible to set up classifiers that correctly identify the samples as either healthy or tumor samples. The spatial resolution of LIMS enables extraction of additional information, such as the existence of necrotic areas or the existence of different tumor cell populations, but such information does not seem determinant for the correct classification of the samples, or it may be somehow compensated by the higher analytical power of HPLC-MS. Similar conclusions were reached with two very different techniques, validating their use for the discovery of lipid biomarkers.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Materials Andmentioning

confidence: 99%

Using the Synergy between HPLC-MS and MALDI-MS Imaging to Explore the Lipidomics of Clear Cell Renal Cell Carcinoma

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“… 9 The sections were scanned in negative-ion mode using the orbitrap analyzer of a MALDI-LTQ-Orbitrap XL (Thermo Fisher, San Jose, CA, USA), equipped with a modified MALDI source. 27 …”

Section: Materials and Methodsmentioning

confidence: 99%

“…Briefly, DAN was used as the matrix for negative-ion detection and was deposited with the aid of our in-house-designed sublimator . The sections were scanned in negative-ion mode using the orbitrap analyzer of a MALDI-LTQ-Orbitrap XL (Thermo Fisher, San Jose, CA, USA), equipped with a modified MALDI source …”

Section: Materials and Methodsmentioning

confidence: 99%

“…9 The sections were scanned in negative-ion mode using the orbitrap analyzer of a MALDI-LTQ-Orbitrap XL (Thermo Fisher, San Jose, CA, USA), equipped with a modified MALDI source. 27 Data were acquired with a mass resolution of 60,000 at m/z = 400. Two microscans of 10 laser shots were recorded for each pixel using a 10 μm raster size.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

See 1 more Smart Citation

High-Resolution Human Kidney Molecular Histology by Imaging Mass Spectrometry of Lipids

et al. 2021

Self Cite

View full text Add to dashboard Cite

For many years, traditional histology has been the gold standard for the diagnosis of many diseases. However, alternative and powerful techniques have appeared in recent years that complement the information extracted from a tissue section. One of the most promising techniques is imaging mass spectrometry applied to lipidomics. Here, we demonstrate the capabilities of this technique to highlight the architectural features of the human kidney at a spatial resolution of 10 μm. Our data demonstrate that up to seven different segments of the nephron and the interstitial tissue can be readily identified in the sections according to their characteristic lipid fingerprints and that such fingerprints are maintained among different individuals ( n = 32). These results set the foundation for further studies on the metabolic bases of the diseases affecting the human kidney.

show abstract

Lipid fingerprint‐based histology accurately classifies nevus, primary melanoma, and metastatic melanoma samples

Huergo‐Baños,

Velasco,

Garate

et al. 2023

Intl Journal of Cancer

Self Cite

View full text Add to dashboard Cite

Probably, the most important factor for the survival of a melanoma patient is early detection and precise diagnosis. Although in most cases these tasks are readily carried out by pathologists and dermatologists, there are still difficult cases in which no consensus among experts is achieved. To deal with such cases, new methodologies are required. Following this motivation, we explore here the use of lipid imaging mass spectrometry as a complementary tool for the aid in the diagnosis. Thus, 53 samples (15 nevus, 24 primary melanomas, and 14 metastasis) were explored with the aid of a mass spectrometer, using negative polarity. The rich lipid fingerprint obtained from the samples allowed us to set up an artificial intelligence‐based classification model that achieved 100% of specificity and precision both in training and validation data sets. A deeper analysis of the image data shows that the technique reports important information on the tumor microenvironment that may give invaluable insights in the prognosis of the lesion, with the correct interpretation.

show abstract

Improving Spatial Resolution of a LTQ Orbitrap MALDI Source

Cited by 5 publications

References 14 publications

Using the Synergy between HPLC-MS and MALDI-MS Imaging to Explore the Lipidomics of Clear Cell Renal Cell Carcinoma

Using the Synergy between HPLC-MS and MALDI-MS Imaging to Explore the Lipidomics of Clear Cell Renal Cell Carcinoma

High-Resolution Human Kidney Molecular Histology by Imaging Mass Spectrometry of Lipids

Lipid fingerprint‐based histology accurately classifies nevus, primary melanoma, and metastatic melanoma samples

Contact Info

Product

Resources

About