Improving Soybean (Glycine max L.) N2 Fixation under Stress

Miransari, Mohammad; Riahi, Hossein; Eftekhar, Fereshteh; Minaie, A.; Smith, Donald L.

doi:10.1007/s00344-013-9335-7

Cited by 46 publications

(26 citation statements)

References 110 publications

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“…For example, fluctuations in pH, nutrient availability, temperature, and salinity, among other environmental stress factors, greatly influences the growth, survival, and metabolic activity of soil microorganisms and plants and their ability to enter into symbiotic interactions. In most cases, the microsymbiont is the more affected partner (Miransari et al, 2013). Therefore, the selection of stress-tolerant strains of Rhizobium are important under stressful environmental conditions (Zahran, 1999).…”

Section: Resultsmentioning

confidence: 99%

Genetic and phenotypic diversity of Rhizobium isolates from Southern Ecuador

et al. 2017

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Rhizobium-legume symbioses play relevant roles in agriculture but have not been well studied in Ecuador. The aim of this study was to characterize the genetic and phenotypic diversity of Rhizobium isolates associated with Phaseolus vulgaris from southern Ecuador. Morphocultural characterization, biochemical tests and physiological analyses were conducted to authenticate and determine the diversity of bacteria Rhizobium-like isolates. The genetic diversity of the isolates was determined by molecular techniques, which consisted of bacteria DNA extraction and amplification and sequencing of the 16S rRNA gene. The nodulation parameters and nitrogen fixation for P. vulgaris under greenhouse conditions were also assessed to determine the phenotypic diversity among isolates. Furthermore, bacteria indole-acetic-acid production was evaluated by the colorimetric method. Morpho-cultural and biochemical characteristic assessments demonstrated that Rhizobium-like bacteria was associated with the P. vulgaris nodules. The diversity among the isolates, as determined by physiological analyses, revealed the potential of several isolates to grow at different pH values, salinity conditions and temperatures. Partial sequencing of the 16S rRNA gene identified the Rhizobium genus in every sampling site. From a total of 20 aligned sequences, nine species of Rhizobium were identified. Nodule formation and biomass, as well as nitrogen fixation, showed an increase in plant phenotypic parameters, which could be influenced by IAA production, especially for the strains R. mesoamericanum NAM1 and R. leguminosarum bv. viciae COL6. These results demonstrated the efficiency of native symbiotic diazotrophic strains inoculants for legume production. This work can serve as the basis for additional studies of native Rhizobium strains and to help spread the use of biofertilizers in Ecuadorian fields.Index terms: Phaseolus vulgaris; diazotrophic bacteria; 16S rRNA gene; nodulation; indole acetic acid. RESUMOA simbiose Rhizobium-leguminosa desempenha um relevante papel na agricultura, entretanto não tem recebido suficiente atenção de estudos científicos no Equador. O objetivo deste artigo foi caracterizar a diversidade genética e fenotípica de isolados de Rhizobium associados com Phaseolus vulgaris do sul do Equador. A caracterização morfo-cultural, testes bioquímicos e análises fisiológicas foram realizados para autenticar e determinar a diversidade de isolados de bactérias Rhizobium. A diversidade genética foi determinada por por técnicas moleculares consistindo na extração de DNA genômico bacteriano, amplificação e sequenciamento parcial do gene 16SrRNA; e parâmetros de nodulação e fixação de nitrogênio de P. vulgaris sobre condições de estufa foram testados para determinar a diversidade fenotípica entre os isolados. Além disso, a produção de ácido indolacético foi avaliada por um método colorimétrico. A análise fisiológica da diversidade entre os isolados revelou o potencial de crescimento de diversos isolados em diferentes níveis de pH, sa...

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Section: Resultsmentioning

confidence: 99%

Genetic and phenotypic diversity of Rhizobium isolates from Southern Ecuador

et al. 2017

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“…Legume roots exclusively contained sequence type 50. These all suggest that legume nodules may have enhancing or inhibiting effects on root colonization of some AM species (Scheublin et al 2004;Miransari et al 2013).…”

Section: Interactions Between Am Fungi and N-fixing Bacteriamentioning

confidence: 95%

“…These are all the likely explanations indicating the different tendency of root nodules for different AM species as all affect the ability of AM species to colonize legume nodules (Scheublin et al 2004;Miransari et al 2013).…”

Section: Interactions Between Am Fungi and N-fixing Bacteriamentioning

confidence: 97%

“…Phylogenically they belong to two subclasses of proteobacteria, metabolically they perform different metabolic processes the most important of which is N fixation, and genetically they include a wide range of bacteria colonizing their specific legume plant. Rhizobia presently include 12 genera and more than 70 species (Masson-Boivin et al 2009;Miransari et al 2013). …”

Section: Diversity and Functionsmentioning

confidence: 99%

“…The interactions between the bacteria and the fungi can occur by the settlement of the bacteria on the fungal hyphae, and be affected by the production of different products by the two microbes such as carbonaceous ones by the fungi. Such kind of interactions can also be influenced by the genetic fluctuations such as horizontal genes transfer between the interactive bacteria and between the bacteria and the host plant (Nazir et al 2010;Miransari 2011b;Miransari et al 2013Miransari et al , 2014b.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Interactions of Soil Microbes, Arbuscular Mycorrhizal Fungi and N-Fixing Bacteria, Rhizobium, Under Different Conditions Including Stress

Miransari¹

2014

Use of Microbes for the Alleviation of Soil Stresses

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Short‐season soybean yield and protein unresponsive to starter nitrogen fertilizer

Brar

Lawley

2020

Agronomy Journal

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The Canadian Prairies have become the new northern edge for soybean [Glycine max (L.) Merr.] production in North America. In this short growing season production area, it has been speculated that application of nitrogen (N) fertilizer at planting could increase soybean growth, yield, and grain protein. To test this hypothesis, field experiments were conducted to determine if starter N fertilizer rates of 0, 17, 34, 50, 67, and 84 kg N ha −1 applied as broadcast and incorporated urea before planting would have an effect on biomass, yield, grain protein and oil, and biological N fixation (BNF) for a Maturity Group 00 soybean cultivar grown in Manitoba, Canada. There was no yield response to starter N fertilizer in 2 of the 3 yr of the study. There were no differences in biomass, grain protein, or oil content in response to starter N fertilizer. Nitrogen uptake at the full bloom stage (R2) increased at the highest N rate tested. Percent BNF as measured by relative ureide N (RUN) content in the N fertilized treatments was reduced in 2 of 3 yr relative to the control. The contributions of BNF, soil N, and fertilizer N to total crop uptake was influenced by growing conditions during the study period. The results of this study do not support the recommendation that starter N fertilizer be used as a way to increase soybean yield and grain protein in the short season soybean production areas of the Canadian Prairies.

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Improving Soybean (Glycine max L.) N2 Fixation under Stress

Cited by 46 publications

References 110 publications

Genetic and phenotypic diversity of Rhizobium isolates from Southern Ecuador

Genetic and phenotypic diversity of Rhizobium isolates from Southern Ecuador

The Interactions of Soil Microbes, Arbuscular Mycorrhizal Fungi and N-Fixing Bacteria, Rhizobium, Under Different Conditions Including Stress

Short‐season soybean yield and protein unresponsive to starter nitrogen fertilizer

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