2001
DOI: 10.1007/s00216-001-1215-5
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Improving selenium extraction by sequential enzymatic processes for Se-speciation of selenium-enriched Agaricus bisporus

Abstract: Sample preparation methods based on the use of proteolytic and cell wall digesting enzymes for the speciation analysis of selenized mushroom were investigated. The sample (Agaricus bisporus; 160 microg total Se per g sample) was grown on compost supplemented with selenized yeast. Experiments were carried out to elucidate the possible role of the cell wall digesting enzymes--Lysing enzyme and Driselase--in the improvement of extraction efficiency with and without inhibiting proteolysis during cell wall digestio… Show more

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Cited by 80 publications
(48 citation statements)
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“…10 In the literature, HPLC is most often used in connection with ICP-MS, 4,[11][12][13][14][15] HG-AAS 16 and HG-AFS. 7,[17][18][19] For separation of volatile selenium species, GC may be used. 20 The aim of this work was to identify and quantify the selenium species in pumpkin seeds enriched in selenium by foliar fertilization, using high performance liquid chromatography-ultraviolet treatment-hydride generation atomic fluorescence spectrometry (HPLC-UV-HG-AFS).…”
mentioning
confidence: 99%
“…10 In the literature, HPLC is most often used in connection with ICP-MS, 4,[11][12][13][14][15] HG-AAS 16 and HG-AFS. 7,[17][18][19] For separation of volatile selenium species, GC may be used. 20 The aim of this work was to identify and quantify the selenium species in pumpkin seeds enriched in selenium by foliar fertilization, using high performance liquid chromatography-ultraviolet treatment-hydride generation atomic fluorescence spectrometry (HPLC-UV-HG-AFS).…”
mentioning
confidence: 99%
“…At this phase in digestion, proteins, polysaccharides and fats are hydrolysed into products that can be absorbed, i.e., surpass the intestinal membrane. Enzymatic digestion procedures replicating intestinal digestion consist of the application of both single enzymes, such as trypsin (Pardo-Martínez, Viñas, Fisher & Hill, 2001;Peña-Farfal et al, 2005), α-amylase (Caruso, Heitkemper & Hymer, 2001;Peña-Farfal et al, 2004 ), lipase (Peña-Farfal et al, 2004) and pronase (Dernovics, Stefánka & Fodor, 2002), or their natural combination, pancreatin (Miller, Schricker, Rasmussen & Van Campen, 1981;Pardo-Martínez, Viñas, Fisher & Hill, 2001;Peña-Farfal et al, 2004;Kulkarni, Acharya, Rajurkar & Reddy, 2007) and synthetic mixtures, such as pancreatin with α-amylase (Azenha & Vasconcelos, 2000;Reyes et al, 2006) or pronase with amylase (Casiot, Szpunar, Łobiński, Potin-Gautier, 1999).…”
Section: Introductionmentioning
confidence: 99%
“…To simulate the gastrointestinal movement and mixture of food during enzymatic hydrolysis, the samples were shaken (Peña-Farfal et al, 2005;Reyes et al, 2006), stirred, either mechanically or magnetically (Ponce de Leon, Sutton, Caruso & Uden, 2000;Caruso, Heitkemper & Hymer, 2001;Kulkarni, Acharya, Rajurkar & Reddy, 2007), or swirled (B'Hymer & Caruso, 2000 for a set period of time primarily in a water bath. Usually, procedures simulating gastric digestion last from 1 to 6 hours (Peña-Farfal et al, 2005;Peña-Farfal et al, 2005;Kulkarni, Acharya, Rajurkar & Reddy, 2007), whereas the samples are incubated in the intestinal juices for 1 to 24 hours (Pardo-Martínez, Viñas, Fisher & Hill, 2001;Dernovics, Stefánka & Fodor, 2002;Peña-Farfal et al, 2004). A distinct reduction of the length of the enzymatic hydrolysis procedure to 30 minutes was achieved using ultrasonic energy (Peña-Farfal et al, 2005).…”
Section: Introductionmentioning
confidence: 99%
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