Improving <scp>SERS</scp> bioimaging of subcutaneous phantom in vivo with optical clearing

Khlebtsov, Boris N.; Буров, А. М.; Pylaev, Timofey; Savkina, Angelina A.; Prikhozhdenko, Ekaterina S.; Bratashov, Daniil; Khlebtsov, Nikolai G.

doi:10.1002/jbio.202100281

Cited by 5 publications

(4 citation statements)

References 44 publications

(47 reference statements)

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“…Other improvements for SERS probes include the application of nanorods, 173–175 nanostars, 176–178 nanoflowers, 179 and core‐shell nanostructures 180–183 . Improving the quality of SERS in vivo imaging by optical clearing is also an acceptable option 184,185 . Since visible light is scattered or absorbed in large amounts, 155 limiting its penetration into the tissue and thus poor imaging, most early SERS detection was based on the first window of NIR with wavelengths of 650–900 nm, mostly 785 nm.…”

Section: Sers‐based Biosensingmentioning

confidence: 99%

“…[180][181][182][183] Improving the quality of SERS in vivo imaging by optical clearing is also an acceptable option. 184,185 Since visible light is scattered or absorbed in large amounts, 155 limiting its penetration into the tissue and thus poor imaging, most early SERS detection was based on the first window of NIR with wavelengths of 650-900 nm, mostly 785 nm. Although the first window of NIR provides more excellent tissue penetration than the visible wavelength, the second window of NIR actually has further reduced scattering, absorption, and background noise compared to the first window due to the longer wavelength.…”

Section: In Vivo Diagnosis and Imagingmentioning

confidence: 99%

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Optical biosensing systems for a biological living body

Yan

Guo

Wang

et al. 2023

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With the development of technology, biosensors are increasingly used in the biomedical field. Due to the high sensitivity of optical signals, good stability, high signal‐to‐noise ratio, and different spectral characteristics of different analytes, optical biosensors can achieve direct and real‐time detection of analytes with high specificity compared with traditional analytical techniques. In view of the rapid development of optical biosensors for in vivo applications and their promising future, we have attempted to summarize the working principles and recent advances in application in humans, with the aim of helping readers to gain an in‐depth and detailed understanding of optical biosensors developed in recent years for applications in the biological living body. In this review, we focus on some of the current widely used and promising optical biosensors, including colorimetric biosensors, fluorescence biosensors, and surface‐enhanced‐Raman‐scattering‐based biosensors. The working principles for each type of optical biosensor are concisely described and the application of the biosensor in the living body is summarized by category. We conclude by looking at the prospects of in vivo applications of optical biosensors.

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Section: Sers‐based Biosensingmentioning

confidence: 99%

Section: In Vivo Diagnosis and Imagingmentioning

confidence: 99%

Optical biosensing systems for a biological living body

Yan

Guo

Wang

et al. 2023

VIEW

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“…2(d) . Skin optical clearing also improved Raman spectroscopy to detect stronger and deeper Raman peaks of skin 68 and assisted surface-enhanced Raman scattering imaging in identifying the shape and boundary of the tumor phantom more accurately, 69 , 70 which would help determine the location of tumors and the presence of metastases. In addition, skin optical clearing increased detection sensitivity of circulating cells by flow cytometry 71 , 72 and improved the signal intensity and imaging depth of laser confocal microscopy when detecting monocytes in mice footpads, 31 as shown in Fig.…”

Section: In Vivo Skin Optical Clearing For Improvement Of Im...mentioning

confidence: 99%

In vivo skin optical clearing for improving imaging and light-induced therapy: a review

Qin

et al. 2023

J. Biomed. Opt.

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.SignificanceSkin is the largest organ and also the first barrier of body. Skin diseases are common, and cutaneous microcirculation is relative to various diseases. Researchers attempt to develop novel imaging techniques to obtain the complex structure, components, and functions of skin. Modern optical techniques provide a powerful tool with non-invasiveness, but the imaging performance suffers from the turbid character of skin. In vivo skin optical clearing technique has been proposed to reduce tissue scattering and enhance penetration depth of light and became a hot topic of research.AimThe aim of this review is to provide a comprehensive overview of recent development of in vivo skin optical clearing methods, how in vivo skin optical clearing enhances imaging performance, and its applications in study and light therapy of various diseases.ApproachBased on the references published over the last decade, the important milestones on the mechanism, methods, and its fundamental and clinical applications of in vivo skin optical clearing technique are provided.ResultsWith the deepening understanding of skin optical clearing mechanisms, efficient in vivo skin optical clearing methods were constantly screened out. These methods have been combined with various optical imaging techniques to improve imaging performances and acquire deeper and finer skin-related information. In addition, in vivo skin optical clearing technique has been widely applied in assisting study of diseases as well as achieving safe, high-efficiency light-induced therapy.ConclusionsIn the last decade, in vivo skin optical clearing technique has developed rapidly and played an important role in skin-related studies.

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“…Therefore, we encounter two opposite demands: (1) to make the light irradiation as safe as possible for healthy organs and (2) to deliver the desired light power to targeted PPT sites. The possible solutions are the use of appropriate light waveguides and optical clearing techniques [268][269][270][271].…”

Section: Perspective and Limitationsmentioning

confidence: 99%

Photothermal and Photodynamic Therapy of Tumors with Plasmonic Nanoparticles: Challenges and Prospects

Bucharskaya

Khlebtsov

et al. 2022

Materials

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Cancer remains one of the leading causes of death in the world. For a number of neoplasms, the efficiency of conventional chemo- and radiation therapies is insufficient because of drug resistance and marked toxicity. Plasmonic photothermal therapy (PPT) using local hyperthermia induced by gold nanoparticles (AuNPs) has recently been extensively explored in tumor treatment. However, despite attractive promises, the current PPT status is limited by laboratory experiments, academic papers, and only a few preclinical studies. Unfortunately, most nanoformulations still share a similar fate: great laboratory promises and fair preclinical trials. This review discusses the current challenges and prospects of plasmonic nanomedicine based on PPT and photodynamic therapy (PDT). We start with consideration of the fundamental principles underlying plasmonic properties of AuNPs to tune their plasmon resonance for the desired NIR-I, NIR-2, and SWIR optical windows. The basic principles for simulation of optical cross-sections and plasmonic heating under CW and pulsed irradiation are discussed. Then, we consider the state-of-the-art methods for wet chemical synthesis of the most popular PPPT AuNPs such as silica/gold nanoshells, Au nanostars, nanorods, and nanocages. The photothermal efficiencies of these nanoparticles are compared, and their applications to current nanomedicine are shortly discussed. In a separate section, we discuss the fabrication of gold and other nanoparticles by the pulsed laser ablation in liquid method. The second part of the review is devoted to our recent experimental results on laser-activated interaction of AuNPs with tumor and healthy tissues and current achievements of other research groups in this application area. The unresolved issues of PPT are the significant accumulation of AuNPs in the organs of the mononuclear phagocyte system, causing potential toxic effects of nanoparticles, and the possibility of tumor recurrence due to the presence of survived tumor cells. The prospective ways of solving these problems are discussed, including developing combined antitumor therapy based on combined PPT and PDT. In the conclusion section, we summarize the most urgent needs of current PPT-based nanomedicine.

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Improving SERS bioimaging of subcutaneous phantom in vivo with optical clearing

Cited by 5 publications

References 44 publications

Optical biosensing systems for a biological living body

Optical biosensing systems for a biological living body

In vivo skin optical clearing for improving imaging and light-induced therapy: a review

Photothermal and Photodynamic Therapy of Tumors with Plasmonic Nanoparticles: Challenges and Prospects

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