Improving rice photosynthesis and yield through trehalose 6-phosphate signaling

Paul, M. J.; Miret, Javier A.; Griffiths, Cara A.

doi:10.1016/j.molp.2022.03.004

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…Recently, it has been discovered that the sugar-inducible transcription factor OsNAC23 can repress OsTPP1 expression, resulting in elevated T6P concentrations and a 13-17% increase in rice yield (Li et al, 2022). This underscores the potential for modifying T6P concentrations to enhance crop yield (Paul et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Trehalose‐6‐phosphate (T6P) is a key signaling molecule in sucrose availability and carbon (C) metabolism (Figueroa & Lunn, 2016; Schluepmann et al., 2004). T6P plays a critical role in regulating sucrose utilization and allocation, and fundamental processes that drive crop growth and yield (Paul et al., 2020; Paul et al., 2022). Notably, transgenic maize plants overexpressing rice OsTPP1 using a floral promoter (MADS6) exhibit reduced T6P concentrations in reproductive tissues, resulting in higher yields under both non‐drought and drought conditions (Nuccio et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

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Trehalose‐6‐phosphate synthase 8 increases photosynthesis and seed yield in Brassica napus

Yuan,

Zhou,

et al. 2024

The Plant Journal

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SUMMARYTrehalose‐6‐phosphate (T6P) functions as a vital proxy for assessing carbohydrate status in plants. While class II T6P synthases (TPS) do not exhibit TPS activity, they are believed to play pivotal regulatory roles in trehalose metabolism. However, their precise functions in carbon metabolism and crop yield have remained largely unknown. Here, BnaC02.TPS8, a class II TPS gene, is shown to be specifically expressed in mature leaves and the developing pod walls of Brassica napus. Overexpression of BnaC02.TPS8 increased photosynthesis and the accumulation of sugars, starch, and biomass compared to wild type. Metabolomic analysis of BnaC02.TPS8 overexpressing lines and CRISPR/Cas9 mutants indicated that BnaC02.TPS8 enhanced the partitioning of photoassimilate into starch and sucrose, as opposed to glycolytic intermediates and organic acids, which might be associated with TPS activity. Furthermore, the overexpression of BnaC02.TPS8 not only increased seed yield but also enhanced seed oil accumulation and improved the oil fatty acid composition in B. napus under both high nitrogen (N) and low N conditions in the field. These results highlight the role of class II TPS in impacting photosynthesis and seed yield of B. napus, and BnaC02.TPS8 emerges as a promising target for improving B. napus seed yield.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Trehalose‐6‐phosphate synthase 8 increases photosynthesis and seed yield in Brassica napus

Yuan,

Zhou,

et al. 2024

The Plant Journal

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“…Because of its effect on plant growth and development, modification of trehalose biosynthesis, either at the level of T6P synthesis, T6P hydrolysis, or trehalose hydrolysis, holds the potential to improve carbon partitioning and thus crop yield and biomass under various stress conditions [ 30 , 31 ]. Ectopic expression of the yeast TPS1 gene in potato, tobacco, and tomato improved drought, salt, and oxidative stress resistance, providing a proof of concept for targeting the trehalose metabolic pathway to improve stress tolerance [ 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Trehalose-6-Phosphate Synthase and Trehalose-6-Phosphate Phosphatase Genes of Tomato (Solanum lycopersicum L.) and Analysis of Their Differential Expression in Response to Temperature

Mollavali

Börnke

2022

IJMS

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In plants, the trehalose biosynthetic pathway plays key roles in the regulation of carbon allocation and stress adaptation. Engineering of the pathway holds great promise to increase the stress resilience of crop plants. The synthesis of trehalose proceeds by a two-step pathway in which a trehalose-phosphate synthase (TPS) uses UDP-glucose and glucose-6-phosphate to produce trehalose-6 phosphate (T6P) that is subsequently dephosphorylated by trehalose-6 phosphate phosphatase (TPP). While plants usually do not accumulate high amounts of trehalose, their genome encodes large families of putative trehalose biosynthesis genes, with many members lacking obvious enzymatic activity. Thus, the function of putative trehalose biosynthetic proteins in plants is only vaguely understood. To gain a deeper insight into the role of trehalose biosynthetic proteins in crops, we assessed the enzymatic activity of the TPS/TPP family from tomato (Solanum lycopersicum L.) and investigated their expression pattern in different tissues as well as in response to temperature shifts. From the 10 TPS isoforms tested, only the 2 proteins belonging to class I showed enzymatic activity, while all 5 TPP isoforms investigated were catalytically active. Most of the TPS/TPP family members showed the highest expression in mature leaves, and promoter–reporter gene studies suggest that the two class I TPS genes have largely overlapping expression patterns within the vasculature, with only subtle differences in expression in fruits and flowers. The majority of tomato TPS/TPP genes were induced by heat stress, and individual family members also responded to cold. This suggests that trehalose biosynthetic pathway genes could play an important role during temperature stress adaptation. In summary, our study represents a further step toward the exploitation of the TPS and TPP gene families for the improvement of tomato stress resistance.

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