Improving Quantitative Measurements for the Evaporative Light Scattering Detector

Mathews, Ben; Higginson, Paul; Lyons, Richard; Mitchell, John C.; Sach, Neal W.; Snowden, Martin J.; Taylor, M.R.S.; Wright, A. G.

doi:10.1365/s10337-004-0441-3

Cited by 87 publications

(43 citation statements)

References 8 publications

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“…at decreasing the time needed for a single HT 2D-LC experiment (methods I-III in Table 2). Yet, less time is available for an individual SEC analysis, which may lead to overlap of two fractions [93][94][95][96]. The theoretical plate number (N) (column efficiency) and the resolution (R) of this column were determined to be 4500 (N 4500 ) and 0.35, respectively (Eqs.…”

Section: Influence Of the Sec Separation And Use Of Ir Detection In 2mentioning

confidence: 99%

Separation of bimodal high density polyethylene using multidimensional high temperature liquid chromatography

Prabhu

Brüll

Macko

et al. 2015

Journal of Chromatography A

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Section: Influence Of the Sec Separation And Use Of Ir Detection In 2mentioning

confidence: 99%

Separation of bimodal high density polyethylene using multidimensional high temperature liquid chromatography

Prabhu

Brüll

Macko

et al. 2015

Journal of Chromatography A

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“…When used in ELSD, solvent gradients can be quite problematic as the response factor may no longer remain constant. As the organic content of the mobile phase increases, the transport efficiency of the nebulizer may increase leading to changes in size and number of droplets carrying the analyte to the detector (35). Nonlinearity of the standard curve is often the result.…”

Section: Column Selection and Chromatographic Conditionsmentioning

confidence: 99%

Development of a Robust Method for Simultaneous Quantification of Polymer (HPMC) and Surfactant (Dodecyl β-D-Maltoside) in Nanosuspensions

Patel

Bummer

2015

AAPS PharmSciTech

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Abstract. This report describes the development of a chromatographic method for the simultaneous quantification of a polymer, hydroxypropyl methylcellulose (HPMC), and a surfactant, dodecyl β-Dmaltoside (DM), that are commonly used in the physical stabilization of pharmaceutical formulations such as nanosuspensions and solid dispersions. These excipients are often challenging to quantify due to the lack of chromophores. A reverse phase size exclusion chromatography (SEC) with evaporative light scattering detector (ELSD) technique was utilized to develop an accurate and robust assay for the simultaneous quantification of HPMC and DM in a nanosuspension formulation. The statistical design of experiments was used to determine the influence of critical ELSD variables including temperature, pressure, and gain on accuracy, precision, and sensitivity of the assay. A robust design space was identified where it was determined that an increase in the temperature of the drift tube and gain of the instrument increased the accuracy and precision of the assay and a decrease in the nebulizer pressure value increased the sensitivity of the assay. In the optimized design space, response data showed that the assay could quantify HPMC and DM simultaneously with good accuracy, precision, and reproducibility. Overall, SEC-ELSD proved to be a powerful technique for the simultaneous quantification of HPMC and DM. This technique can be used to quantify the amount of HPMC and DM in nanosuspensions, which is critical to understanding their effects on the physical stability of nanosuspensions.KEY WORDS: design of experiments; dodecyl β-D-maltoside; evaporative light scattering detector; HPMC; nanosuspensions.

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“…In the course of gradient-elution runs the ELSD response was found to ameliorate by gradually passing to less aqueous containing eluents [9,76]. The progressive variation of the eluent physico-chemical properties (in terms of surface tension, viscosity and density) mainly results in a better nebulization event [9,76]. According to Mathews et al [76], although the average particle size remains constant, a superior amount of them enters the detection chamber, thus augmenting in turn the signal.…”

Section: Lods and Loqsmentioning

confidence: 99%

“…Indeed, as reported in a previous section, an elevated MeCN content (90%) has been necessary to get the epimeric resolution in a reasonable analysis time. In the course of gradient-elution runs the ELSD response was found to ameliorate by gradually passing to less aqueous containing eluents [9,76]. The progressive variation of the eluent physico-chemical properties (in terms of surface tension, viscosity and density) mainly results in a better nebulization event [9,76].…”

Section: Lods and Loqsmentioning

confidence: 99%

“…The progressive variation of the eluent physico-chemical properties (in terms of surface tension, viscosity and density) mainly results in a better nebulization event [9,76]. According to Mathews et al [76], although the average particle size remains constant, a superior amount of them enters the detection chamber, thus augmenting in turn the signal. However, as detailed above, the eluent composition can be crucially responsible for the droplet size distribution and hence for the overall light scattering process [77].…”

Section: Lods and Loqsmentioning

confidence: 99%

See 1 more Smart Citation

Side‐chain modified bile acids: chromatographic separation of 23‐methyl epimers

Natalini¹,

Sardella

Gioiello

et al. 2009

J of Separation Science

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Owing to the re-flourished interest towards the bile acids (BAs) as versatile signalling hormones endowed with diverse endocrine functions, the development of reliable analytical protocols monitoring the synthesis of new BA-based receptor modulators, still represents a cogent concern. On this basis, for the first time, a HPLC study has been engaged with the aim to set up suitable chromatographic conditions for the analysis of three different epimeric couples of 23-methyl-substituted unconjugated BAs. Three different methods (one for each couple) have been successfully established and then validated. Good precision and accuracy (evaluated both in the short and long period) as well as appreciably low LOD and LOQ values have turned out. Moreover, the engagement of an evaporative light scattering detector (ELSD) has proven its high effectiveness for the analysis of such steroidal species.

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Improving Quantitative Measurements for the Evaporative Light Scattering Detector

Cited by 87 publications

References 8 publications

Separation of bimodal high density polyethylene using multidimensional high temperature liquid chromatography

Separation of bimodal high density polyethylene using multidimensional high temperature liquid chromatography

Development of a Robust Method for Simultaneous Quantification of Polymer (HPMC) and Surfactant (Dodecyl β-D-Maltoside) in Nanosuspensions

Side‐chain modified bile acids: chromatographic separation of 23‐methyl epimers

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