Improving process conditions of hydroxytyrosol synthesis by toluene-4-monooxygenase

Brouk, Moran; Fishman, Ayelet

doi:10.1016/j.molcatb.2012.05.010

Cited by 20 publications

(11 citation statements)

References 28 publications

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“…By subjecting T4MO to further rounds of directed evolution coupled with statistical analysis the authors were able to identify a variant I100A/E214G/D285Q with a 190‐fold increase in 2‐phenylethanol oxidation 31…”

Section: Sdimo Enzymes – Diversity and Mutagenesis Studiesmentioning

confidence: 99%

Tailoring the Morphology of g‐C₃N₄ by Self‐Assembly towards High Photocatalytic Performance

et al. 2014

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We report a novel method for the preparation of graphitic carbon nitride (g‐C3N4) with various morphologies through self‐assembly and calcination, which starts from the raw materials melamine, urea, and cyanuric acid. The hollow to wormlike morphologies of g‐C3N4 could be readily tailored by adjusting the molar ratio of melamine to urea; with increase in the molar ratio from 3:1 to 1:3, a morphology transformation was observed. The morphologies were tailored by self‐assembly of the aggregates by hydrogen bonding and ionic interactions. Correspondingly, an increased BET surface area from 49.6 to 97.4 m2 g−1 was observed. If used as a photocatalyst in degrading rhodamine B (RhB) under visible‐light irradiation, these g‐C3N4 samples demonstrated 7 to 13 times higher performance than conventional bulk g‐C3N4. The high performance was attributed to the unique morphology that provided not only high specific surface area but low recombination losses of photogenerated charges.

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Section: Sdimo Enzymes – Diversity and Mutagenesis Studiesmentioning

confidence: 99%

Tailoring the Morphology of g‐C₃N₄ by Self‐Assembly towards High Photocatalytic Performance

et al. 2014

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“…Other mutagenic studies of the SDIMOs that have resulted in increased activity and/or catalytic precision have included a study that increased activity of T4MO for synthesis of polyhydroxylated compounds including the food additive hydroxytyrosol (Brouk et al. 2010 ; Brouk and Fishman 2012 ), increasing enantiomeric excess for the production of chiral sulfoxides (Feingersch et al. 2008 ).…”

Section: Discussionmentioning

confidence: 99%

Mutagenesis and expression of methane monooxygenase to alter regioselectivity with aromatic substrates

Lock

Nichol

Murrell

et al. 2017

FEMS Microbiology Letters

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Soluble methane monooxygenase (sMMO) from methane-oxidising bacteria can oxygenate more than 100 hydrocarbons and is one of the most catalytically versatile biological oxidation catalysts. Expression of recombinant sMMO has to date not been achieved in Escherichia coli and so an alternative expression system must be used to manipulate it genetically. Here we report substantial improvements to the previously described system for mutagenesis of sMMO and expression of recombinant enzymes in a methanotroph (Methylosinus trichosporium OB3b) expression system. This system has been utilised to make a number of new mutants and to engineer sMMO to increase its catalytic precision with a specific substrate whilst increasing activity by up to 6-fold. These results are the first ‘proof-of-principle’ experiments illustrating the feasibility of developing sMMO-derived catalysts for diverse applications.

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“…Toluene-4-monooxygenase from Pseudomonas mendocina KR1 can convert 2phenylethanol into hydroxytyrosol. 20 Orenes-Pinẽro et al transferred the phenol hydroxylase gene from Geobacillus thermoglucosidasius into Escherichia coli to transform tyrosol into hydroxytyrosol. 21 Satoh et al cloned tyrosine hydroxylase from mouse into E. coli to convert tyrosine to hydroxytyrosol.…”

Section: ■ Introductionmentioning

confidence: 99%

“…In addition, Serratia marcescens, Pseudomonas aeruginosa, and Pseudomonas putida F6 can convert tyrosol into hydroxytyrosol. Toluene-4-monooxygenase from Pseudomonas mendocina KR1 can convert 2-phenylethanol into hydroxytyrosol . Orenes-Piñero et al transferred the phenol hydroxylase gene from Geobacillus thermoglucosidasius into Escherichia coli to transform tyrosol into hydroxytyrosol .…”

Section: Introductionmentioning

confidence: 99%

Efficient Synthesis of Hydroxytyrosol from l-3,4-Dihydroxyphenylalanine Using Engineered Escherichia coli Whole Cells

Jia

Bai

et al. 2019

J. Agric. Food Chem.

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Hydroxytyrosol is a high-value-added compound with a variety of biological and pharmacological activities. In this study, a whole-cell catalytic method for the synthesis of hydroxytyrosol was developed: aromatic amino acid aminotransferase (TyrB), L-glutamate dehydrogenase (GDH), α-keto acid decarboxylase (PmKDC), and aldehyde reductase (YahK) were co-expressed in Escherichia coli to catalyze the synthesis of hydroxytyrosol from L-3,4-dihydroxyphenylalanine (L-DOPA). The plasmids with different copy numbers were used to balance the expression of the four enzymes, and the most appropriate strain (pRSF-yahK-tyrB and pCDF-gdh-Pmkdc) was identified. After determination of the optimum temperature (35 °C) and pH (7.5) for whole-cell catalysis, the yield of hydroxytyrosol reached 36.33 mM (5.59 g/L) and the space-time yield reached 0.70 g L −1 h −1 .

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Improving process conditions of hydroxytyrosol synthesis by toluene-4-monooxygenase

Cited by 20 publications

References 28 publications

Tailoring the Morphology of g‐C₃N₄ by Self‐Assembly towards High Photocatalytic Performance

Tailoring the Morphology of g‐C₃N₄ by Self‐Assembly towards High Photocatalytic Performance

Mutagenesis and expression of methane monooxygenase to alter regioselectivity with aromatic substrates

Efficient Synthesis of Hydroxytyrosol from l-3,4-Dihydroxyphenylalanine Using Engineered Escherichia coli Whole Cells

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Improving process conditions of hydroxytyrosol synthesis by toluene-4-monooxygenase

Cited by 20 publications

References 28 publications

Tailoring the Morphology of g‐C3N4 by Self‐Assembly towards High Photocatalytic Performance

Tailoring the Morphology of g‐C3N4 by Self‐Assembly towards High Photocatalytic Performance

Mutagenesis and expression of methane monooxygenase to alter regioselectivity with aromatic substrates

Efficient Synthesis of Hydroxytyrosol from l-3,4-Dihydroxyphenylalanine Using Engineered Escherichia coli Whole Cells

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Tailoring the Morphology of g‐C₃N₄ by Self‐Assembly towards High Photocatalytic Performance

Tailoring the Morphology of g‐C₃N₄ by Self‐Assembly towards High Photocatalytic Performance