Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor

Kasuga, Mie; Li, Qiang; Miura, Setsuko; Yamaguchi-Shinozaki, Kazuko; Shinozaki, Kazuo

doi:10.1038/7036

Cited by 1,843 publications

(1,406 citation statements)

References 33 publications

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“…Among the DRE binding proteins, the DREB2 subfamily has been reported to be induced by drought and high salinity stress. Transgenic Arabidopsis plants expressing DREB1 B/ CBF1 or DREB1 A/CBF3 under the control of CaMV 35S promoter showed strong tolerance to freezing, drought and high salinity stresses suggesting that DREBs/CBFs target multiple genes (Jaglo-Ottosen et al 1998;Liu et al 1998;Kasuga et al 1999). Microarray analyses have indicated that these factors target stress inducible genes containing the conserved DRE or DRE-related core motifs in their promoter regions (Maruyama et al 2004).…”

Section: Resultsmentioning

confidence: 99%

“…Drought tolerance is a complex trait that is being influenced by multiple genes, interactions among genes and environmental cues (Tardieu and Tuberosa 2010). Perception of stress signals and activation of complex signaling pathways bring about drastic changes in the cellular gene expression which is a prerequisite for plants to acclimatize under extreme conditions (Kasuga et al 1999;Tong et al 2007). It is well established that almost all biotic and abiotic stresses induce oxidative stress by producing higher levels of reactive oxygen species (ROS) such as hydrogen peroxide (H 2 O 2 ), superoxide anion (O 2…”

Section: Introductionmentioning

confidence: 99%

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Quantitative expression analysis of drought responsive genes in clones of Hevea with varying levels of drought tolerance

Luke

Sathik

Thomas

et al. 2015

Physiol Mol Biol Plants

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In order to meet the ever rising global demand for natural rubber, cultivation of Hevea is being extended to nontraditional regions of India where extreme climatic conditions like drought and low temperature negatively influence the crop performance. In order to ensure maximum productivity, identification of drought tolerant clones of Hevea which can cope up with stress and give better crop yield is essential. Several attempts have been made previously to identify genes that are associated with drought tolerance in Hevea. In the present study, quantitative expression analysis was made using quantitative PCR for seven drought associated transcripts in four clones of Hevea with varying levels of drought tolerance. Among the seven genes studied, Mitogen Activated Protein (MAP) kinase, Myeloblastosis (Myb) transcription factor, C-repeat responsive element/Dehydration Responsive Element (CRT/DRE) binding factor and Nuclear Factor Y subunit A (NFYA) showed a positive association with drought tolerance. Transcripts of ascorbate peroxidase and heat shock protein 70 (HSP 70) did not show any correlation with drought tolerance. Interestingly, catalase gene was found down regulated in all the clones under drought condition. The possible role of these genes based on their level of gene expression in four different clones of Hevea with varying levels of drought tolerance is discussed.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantitative expression analysis of drought responsive genes in clones of Hevea with varying levels of drought tolerance

Luke

Sathik

Thomas

et al. 2015

Physiol Mol Biol Plants

View full text Add to dashboard Cite

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“…Another important aspect involves the regulated and targeted expression of transgenes, such as by use of stress regulated promoters e.g., rd29 (Kasuga et al, 1999), 6XARE (Rahman et al, 2001), 4ABRC-Act1-100P-Hva22 (Garg et al, 2002;Roy and Wu, 2002;Rohila et al, 2002), AIPC (ABA-induced promoter complex, Zhu et al, 1998), Gmhsp17.6-L (Lee andSchoffl, 1996). AIPC (ABA induced promoter complex) has also been used in conferring salt and water stress tolerance by engineering P5CS in rice .…”

Section: Prospects For Genetic Engineeringmentioning

confidence: 99%

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Khurana

Vishnudasan

Chhibbar

2008

Physiol Mol Biol Plants

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Water deficit arises as a result of low temperature, salinity and dehydration, thereby affecting plant growth adversely and making it imperative for plants to surmount such situations by acclimatizing/adapting at various levels. Water deficit stress results in significant changes in gene expression, mediated by interconnected signal transduction pathways that may be triggered by calcium, and regulated via ABA dependent and/or independent pathways. Hence, adaptation of plants to such stresses involves maintaining cellular homeostasis, detoxification of harmful elements and also growth alterations. Stress in general cause excess production of reactive oxygen species (ROS) and the plants overcome the same by either preventing the accumulation of ROS or by eliminating the ROS formed. Ion homeostasis includes processes such as cellular uptake, sequestration and export in conjunction with long distance transport. Requisite amounts of osmolytes are hence synthesized under stress to maintain turgor along with maintaining the macromolecular structures and also for scavenging ROS. Another noteworthy response is the accumulation of novel proteins, including enzymes involved in the biosynthesis of osmoprotectants, heat-shock proteins (HSPs), late embryogenesis abundant (LEA) proteins, antifreeze proteins, chaperones, detoxification enzymes, transcription factors, kinases and phosphatases. The LEAs belong to a redundant protein family and are highly hydrophilic, boiling-soluble, non-globular and therefore have been defined and classified accordingly. The precise function of LEAs is still unknown, but substantial evidence indicates their involvement in dessication tolerance as the expression of LEAs confers increased resistance to stress in heterologous yeast system and also significantly improves water deficit tolerance in transgenic plants. Genetic manipulation of plants towards conferring abiotic stress tolerance is a daunting task, as the abiotic stress tolerance mechanism is highly complex and various strategies have been exploited to address and evaluate the stress tolerance mechanism, and the molecular responses to water deficit via complex signaling networks. Genomic technologies have recently been useful in integrating the multigenicity of the plant stress responses through, transcriptomics, proteomics and metabolite profilling and their interactions. This review deals with the recent developments on genetic approaches for water stress tolerance in plants, with special emphasis on LEAs.

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“…Just 1 year ago, European Commission, who once kept conservative to biotechnological breeding, constructed a big project: Plants for the Future: a European vision for Plant Biotechnology towards 2025, in which much is involved in resistance drought [14]. Many advances in relation to this hot topic, including molecular mechanism of anti-drought and corresponding molecular breeding have taken place [28,33,34,39,44,47,50,53,[55][56]63,[72][73][74][75]78]. Although the obtained transgenic crops (mainly, wheat) by different types of gene technology all exhibit drought resistance to some extent, they have many shortfalls related to agronomical performance and/or development [47,49,53,62,66,68,74].…”

Section: Introductionmentioning

confidence: 99%

Relationship between water use efficiency (WUE) and production of different wheat genotypes at soil water deficit

Shao

Chu

et al. 2006

Colloids and Surfaces B: Biointerfaces

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Through 2-year field experiments, 7 wheat genotypes were better in their field yield. These 7 wheat genotypes and other 3 wheat species, which are being popularized on a large scale in different locations of China, were selected as experimental materials for the sake of measuring their difference in WUE and production and comparing their relationship at soil water deficits, future more, providing better drought resistance lines and theoretical guide for wheat production and practices and exploring anti-drought physiological mechanisms of different wheat genotypes. Under the condition of 3 soil-water-stress treatments (75% field capacity (FC), 55% FC, 45% FC, named level 1, level 2 and level 3, respectively), pot experiments for them were conducted and the related data were collected from their life circle. The main results were as followed: (1) according to the selected soil stress levels, water use efficiency (WUE) of 10 different wheat genotypes was divided into two groups (A and B); group A included genotypes 2, 3, 4, 5, 6, 7, 8, whose WUE decreased basically from level 1 to level 3 and reached individual peak of WUE at level 1; Group 2 included genotypes 1, 9, 10, whose WUE reached their individual peak at level 2; (2) based on total water consumption through all life circle, genotypes 1, 4, 8, 9 had lower water consumption (TWC) at level 1, genotypes 2, 3, 5, 6, 7 lower TWC at level 2, genotype 10 lower TWC at level 3; (3) at level 1, genotypes 2, 3, 4, 5, 6, 7, 8 had higher grain weight of single spike (GWSS), genotypes 1, 9, 10 better GWSS at level 2, which was in good line with individual WUE of different wheat genotypes; (4) by analyzing the indexes related to examining cultivars, it was found that genotypes 1, 2, 3, 4, 5, 6, 9, 10 had longer plant length (PL), spike length (SL), bigger grain number (GN) except genotypes 7 and 8 at level 1, RL was in better line with genotypes 1, 2, 3, 8, 9, 10, but not in the other genotypes at level 1.

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Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor

Cited by 1,843 publications

References 33 publications

Quantitative expression analysis of drought responsive genes in clones of Hevea with varying levels of drought tolerance

Quantitative expression analysis of drought responsive genes in clones of Hevea with varying levels of drought tolerance

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Relationship between water use efficiency (WUE) and production of different wheat genotypes at soil water deficit

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