Improving Phase Contrast of Digital Holographic Microscope using Spatial Light Modulator

Abstract

We propose a new method for improving the phase contrast of a multiphase digital holographic microscope using a spatial light modulator (SLM). Using the SLM as the annulus, our method improves the light contrast of the object edge to achieve higher accuracy. We demonstrate a digital holographic microscopy technique that provides a 30% improvement in the phase contrast compared to conventional microscopy, which utilizes a mechanical annulus. The phase-contrast improvement allows the 3D reconstructed hologram to be determined more precisely.

Roadmap on Recent Progress in FINCH Technology

“…When the FINCH is applied to the holographic 3D display, it can be an effective way to reconstruct 3D images for living cells where a femtosecond laser is utilized as a laser illumination [100]. The feature of a femtosecond laser is a high level of light intensity, the extreme stability of the optical setup, and the supply of narrow bandwidth light.…”

Sources and propagation of errors in quantitative phase imaging techniques using optical interferometry

“…The interferometric setup may be optimized by reducing the recording distance and using a detector with smaller pixel sizes [8]. Other methods to reduce noise include phase error compensation, spatial light modulation (SLM), and multiple frequency overlapping [9] [10][11][12][13], which improve the contrast of phase measurements. Noise reduction is also accomplished by filtering certain frequencies, both in the spatial and Fourier planes, with Butterworth filters and masks, respectively [14,15] [6].…”