Improving packed red blood cell storage with a high-viscosity buffered storage solution

Pulliam, Kasiemobi E.; Joseph, Bernadin; Makley, Amy T.; Caldwell, Charles C.; Lentsch, Alex B.; Goodman, Michael D.; Pritts, Timothy A.

doi:10.1016/j.surg.2021.11.020

Cited by 6 publications

(4 citation statements)

References 54 publications

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“…The venous blood underwent centrifugation to produce an erythrocyte suspension with isotonic PBS buffer, which was then refrigerated at 4 • C [10,24,25]. To make hardened erythrocytes, erythrocyte samples stored for 1 day (1D) and 7 days (7D) were selected for control tests [26,27]. A 1 mL erythrocyte suspension containing 10 7 cells was diluted to 10 mL with PBS buffer.…”

Section: Preparation Of the Experimentsmentioning

confidence: 99%

Diseased Erythrocyte Enrichment Based on I-Shaped Pillar DLD Arrays

Lv,

Wu,

et al. 2024

Micromachines

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Enrichment of erythrocytes is a necessary step in the diagnosis of blood diseases. Due to the high deformability and viscosity of erythrocytes, they cannot be regarded as stable point-like solids, so the influence of their deformability on fluid dynamics must be considered. Therefore, by using the special effect of an I-shaped pillar (I-pillar) on erythrocytes, erythrocytes with different deformability can be made to produce different provisional distances in the chip, so as to achieve the separation of the two kinds of erythrocytes. In this study, a microfluidic chip was designed to conduct a control test between erythrocytes stored for a long time and fresh erythrocytes. At a specific flow rate, the different deformable erythrocytes in the chip move in different paths. Then, the influence of erythrocyte deformability on its movement trajectory was analyzed by two-dimensional finite element flow simulation. DLD sorting technology provides a new method for the sorting and enrichment of diseased erythrocytes.

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Section: Preparation Of the Experimentsmentioning

confidence: 99%

Diseased Erythrocyte Enrichment Based on I-Shaped Pillar DLD Arrays

Lv,

Wu,

et al. 2024

Micromachines

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“…106,107 Efforts to blunt the effect of storage age on the inflammatory response after transfusion are ongoing and seek to maximize the quality of blood products used for resuscitation. 108…”

Section: Clinical and Therapeutic Implicationsmentioning

confidence: 99%

“…Additional studies suggest that increased systemic inflammation, endothelial cell activation, and microthrombi formation also occur 106,107 . Efforts to blunt the effect of storage age on the inflammatory response after transfusion are ongoing and seek to maximize the quality of blood products used for resuscitation 108 …”

Section: Clinical and Therapeutic Implicationsmentioning

confidence: 99%

The intersection of coagulation activation and inflammation after injury: What you need to know

Costantini,

Kornblith,

Pritts

et al. 2023

J Trauma Acute Care Surg

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The systemic inflammatory response syndrome (SIRS) after severe injury can lead to distant organ injury, multi-organ failure, and complications during recovery. Post-injury SIRS is driven by the activation of innate immune cells and release of pro-inflammatory cytokines that drives this inflammation response. In addition, the coagulation cascade and complement system is altered, resulting in a widespread inflammatory response. Importantly, these different components of SIRS are interrelated and propagate further alterations in thrombosis and inflammation. Efforts to mitigate the acute changes in coagulation and inflammation and its complex interactions following injury could provide novel strategies for resuscitation and management of complications of trauma-induced coagulopathy, SIRS, and multiple organ failure. In this review, we review the pathophysiology of post-traumatic SIRS and highlight current approaches to mitigate innate immune cell activation, thromboinflammation, and associated clinical complications. Level of Evidence IV, Review article

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“…Preservative solutions used to maintain RBC integrity, mitigate the storage lesion, improve RBC recovery, and reduce potential adverse outcomes of PRBC transfusion are constantly evolving. Existing literature reports that significantly less sREVs are generated when PRBCs are stored in saline-adenine-glucose-mannitol (SAGM) vs. phosphate-buffered-saline [10], in additive solution 7 vs. AS1 [17] or in EAS-1587 (a novel additive solution with increased viscosity in the storage medium) vs. AS3 [18 ▪ ]. These findings suggest that additive solution formulations with high nutrient content, high range and capacity of pH buffering, and high viscosity may decrease sREV release during PRBC storage, along with a general improvement of other changes associated with the storage lesion.…”

Section: Generation and Characterization Of Stored Red Cell Derived E...mentioning

confidence: 99%

Red cell extracellular vesicles and coagulation activation pathways

Noubouossie,

Key

2023

Current Opinion in Hematology

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Purpose of review Packed red blood cells (PRBCs) are the most commonly transfused blood products. Preparation of PRBCs requires blood collection from donors, processing, and storage prior to transfusion to recipients. Stored red blood cells (RBCs) undergo structural and metabolic changes collectively known as the storage lesion. RBC extracellular vesicles (sREVs) are released in PRBC units during storage, and are transfused along with intact RBCs into recipients. For several decades, extracellular vesicles have been the focus of intense research, leading to the discovery of a wide variety of endogenous biological properties that may impact numerous physiologic and/or pathologic pathways. Recent findings This study reviews the characteristics of extracellular vesicles present in PRBC units and the impact of prestorage and pretransfusion processing, as well as storage conditions, on their generation. Importantly, we discuss recently described interactions of sREVs with coagulation pathways and related interplay with inflammatory pathways in vitro and in vivo using animal models. Summary Extracellular vesicles present in stored PRBC units are capable of activating coagulation pathways. However, it remains unclear whether this affects clinical outcomes in recipients of PRBC units. Further understanding of these pathways and their relationship to any adverse outcomes may yield novel strategies to mitigate complications of blood transfusion.

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Improving packed red blood cell storage with a high-viscosity buffered storage solution

Cited by 6 publications

References 54 publications

Diseased Erythrocyte Enrichment Based on I-Shaped Pillar DLD Arrays

Diseased Erythrocyte Enrichment Based on I-Shaped Pillar DLD Arrays

The intersection of coagulation activation and inflammation after injury: What you need to know

Red cell extracellular vesicles and coagulation activation pathways

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