Improving ovarian tissue cryopreservation for oncologic patients: slow freezing versus vitrification, effect of different procedures and devices

Herraiz, Sonia; Novella-Maestre, Edurne; Rodríguez, Beatriz García; Díaz, Carmen Pérez; Sánchez-Serrano, María; Mirabet, Vicente; Pellicer, António

doi:10.1016/j.fertnstert.2013.11.016

Cited by 85 publications

(67 citation statements)

References 50 publications

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“…For vitrification, the oocytes are rapidly cooled to − 196°C and stored in liquid nitrogen (also known as shock freezing). Vitrification can be done using either an open system (direct contact between oocytes and liquid nitrogen) or a closed system (oocytes separated from the liquid nitrogen by a membrane) [37]. A systematic review by Edgar et al compared slow freezing with vitrification and came to the conclusion that vitrification was associated with better oocyte survival and better development of the embryo.…”

Section: Cryopreservation Of Oocytesmentioning

confidence: 99%

“…Cytostatic drugs are associated with increasing fibrosis of the ovaries, leading to a decrease in the number of granulosa cells and primordial follicles [5,7]. Irradiation of brain tissue with more than [35][36][37][38][39][40] Gy results in most cases in damage to the hypothalamic-pituitary-adrenal axis and hypogonadism [8]. Irradiation of the pelvis promotes fibrosis of the uterus.…”

mentioning

confidence: 99%

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Fertility Protection in Female Oncology Patients: How Should Patients Be Counseled?

Findeklee

Lotz

Hoffmann

et al. 2015

Geburtshilfe Frauenheilkd

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Protecting the fertility of patients with oncologic disease is becoming more and more important, as fulfilling the wish to have children is increasingly occurring at a later stage in life and long-term survival rates after cancer are continuing to improve. A number of fertility-preserving options exist. In addition to techniques which have been around for some time such as medical ovarian suppression, ovarian transposition, and organ-preserving surgery, there are other, more recent, innovative methods which have developed over the last few years such as cryopreservation of oocytes or ovarian tissue transplantation after completing cancer therapy. As every procedure has its specific advantages and disadvantages, informed patient consent is essential. The physician?s aim must be to select the optimal procedure for each patient. The extent of patients? information about the options to preserve fertility in women with oncologic disease remains limited. One of the main reasons for this is that clinicians are not sure how to inform patients about existing procedures and methods. The aim of this review article is to provide help in clinical practice.

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Section: Cryopreservation Of Oocytesmentioning

confidence: 99%

mentioning

confidence: 99%

Fertility Protection in Female Oncology Patients: How Should Patients Be Counseled?

Findeklee

Lotz

Hoffmann

et al. 2015

Geburtshilfe Frauenheilkd

View full text Add to dashboard Cite

show abstract

“…Preparation of the ovarian tissue, cryopreservation and thawing must ensure follicle viability and integrity of tissue compartments and cell-to cell contacts [ 7 ]. Two well-established methods can be used: rapid vitrifi cation and slow freezing of fi nely dissected thin slices of ovarian cortex tissue [ 8 -11 ].…”

Section: Processing the Ovarian Tissuementioning

confidence: 99%

Ovarian Tissue Cryopreservation

Fatum

McVeigh

2015

Reproductive Surgery in Assisted Conception

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“…Vitrification is already widely and successfully used to cryopreserve embryos and oocytes [14]. Although still considered experimental for ovarian tissue, it is looking increasingly promising [15][16][17][18] and offers an alternative means of cryopreserving ovarian tissue, even if almost all pregnancies to date have been achieved after slow-freezing and grafting [2]. So far, two live births have been obtained after vitrification [4,5].…”

Section: Introductionmentioning

confidence: 99%

Impact of the cryopreservation technique and vascular bed on ovarian tissue transplantation in cynomolgus monkeys

Dolmans

Binda

Jacobs

et al. 2015

J Assist Reprod Genet

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Purpose The aim of this study was to determine the best combination in terms of cryopreservation techniques and vascular bed preparation before grafting in order to obtain functional ovarian tissue after transplantation. Methods Five cynomolgus monkeys were used. Strips from 10 ovaries were cryopreserved, 5 by vitrification (V), and 5 by slow-freezing (SF). Pieces of fresh ovarian tissue were used for controls. After 1 month, the strips were autografted to two different vascular beds, healed (HB) or freshly decorticated (FDB), constituting four study groups: SF-HB, SF-FDB, V-HB, and V-FDB. These were compared to fresh tissue. After 6 months, the ovaries were removed and several parameters analyzed: follicle quality, stage, density, proliferation, apoptosis, functionality, vascularization, and fibrosis. Mixed effect linear regression models were built to assess the impact of cryopreservation and vascular bed preparation on ovarian tissue viability and functionality. p values were adjusted for multiple testing using the Benjamini-Hochberg method, and q values<0.20 were considered significant in order to achieve a 20 % false discovery rate. Results Compared to fresh tissue, no difference was observed in the percentage of morphologically normal follicles, while a significant increase was noted in the follicle proliferation rate (41 %, q=0.19), percentage of antral follicles (12 %, q=0.14), and number of vessels per area (3.3 times, q=0.07) in the V-FDB group. Conclusions Vitrification associated with FDB vascular bed preparation is the best combination to obtain functional autografted ovarian tissue. Further studies are nevertheless required, with confirmed pregnancies and live births before introducing the procedure into clinical practice.Capsule Vitrification associated with freshly decorticated vascular bed preparation appears to be the best combination to obtain functional autografted ovarian tissue. M. M. Dolmans and M. M. Binda contributed equally to this work.Electronic supplementary material The online version of this article

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Improving ovarian tissue cryopreservation for oncologic patients: slow freezing versus vitrification, effect of different procedures and devices

Cited by 85 publications

References 50 publications

Fertility Protection in Female Oncology Patients: How Should Patients Be Counseled?

Fertility Protection in Female Oncology Patients: How Should Patients Be Counseled?

Ovarian Tissue Cryopreservation

Impact of the cryopreservation technique and vascular bed on ovarian tissue transplantation in cynomolgus monkeys

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