Improving osteogenesis of PLGA/HA porous scaffolds based on dual delivery of BMP-2 and IGF-1<i>via</i>a polydopamine coating

Zhang, Jun; Li, Jianan; Jia, Guanghong; Jiang, Yikun; Liu, Qinyi; Yang, Xiaoyu; S, Pan

doi:10.1039/c7ra12062a

Cited by 62 publications

(78 citation statements)

References 43 publications

(52 reference statements)

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“…Although it might avoid the excessive burst release of BMP-2 in this biodegradable delivery system, gelatin microspheres in our study could degrade earlier than PLGA microspheres, and porosity created by degradation of gelatin microspheres in the PLGA matrix would be useful for bone healing [38]. By surface binding technology such as polydopamine-assisted immobilization of BMP-2, some researchers found it can effectively enhance BMP-2 binding on the surface of the PLGA scaffold, and the BMP-2 can be slowly released over three weeks in vitro [14]. However, poor biocompatibility in vitro and insufficient release time in vivo were observed, which was not sufficient to achieve complete defect filling [46].…”

Section: Cell Proliferation and Differentiation In Plga Plga/gms Andmentioning

confidence: 76%

“…Consequently, the enrichment of CPC/PLGA with osteoinductive factors is necessary to improve their biological performance. PLGA/HA scaffolds combined with osteoinductive growth factor via polydopamine were fabricated by some studies, but based on its high porosity, the low mechanical strength, which can influence osteogenesis, has yet to be solved [14]. A wide number of PLGA composite in combination with gelatin (PLGA/gelatin) are being studied as a new type of alternatives for bone tissue regeneration [15].…”

Section: Introductionmentioning

confidence: 99%

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BMP-2-releasing gelatin microspheres/PLGA scaffolds for bone repairment of X-ray-radiated rabbit radius defects

Xia

Wang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Section: Cell Proliferation and Differentiation In Plga Plga/gms Andmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

BMP-2-releasing gelatin microspheres/PLGA scaffolds for bone repairment of X-ray-radiated rabbit radius defects

Xia

Wang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…[7][8][9][10] Recent studies have shown that the combined delivery of BMP-2 and IGF-1 enhances tissue regeneration compared to single growth factor delivery. [11] Lu et al investigated the use of biodegradable hydrogel composite scaffolds to deliver BMP-2 and IGF-1 for the repair of osteochondral tissue in a rabbit model. [12] Their results suggested that the fast release of BMP-2 followed by a slow and sustained release of IGF-1 synergistically enhance the degree of subchondral bone formation.…”

Section: A Poly(lactic-co-glycolic Acid)-poly(ethylene Glycol)-carboxmentioning

confidence: 99%

Development of PLGA‐PEG‐COOH and Gelatin‐Based Microparticles Dual Delivery System and E‐Beam Sterilization Effects for Controlled Release of BMP‐2 and IGF‐1

Bai

Moeinzadeh

Kim

et al. 2020

Part & Part Syst Charact

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A poly(lactic‐co‐glycolic acid)‐poly(ethylene glycol)‐carboxyl (PLGA‐PEG‐COOH) and gelatin‐based microparticles (MPs) dual delivery system for release of bone morphogenetic protein‐2 (BMP‐2) and insulin‐like growth factor‐1 (IGF‐1) is developed. The delivery system is characterized based on its morphology, loading capacity, encapsulation efficiency, and release kinetics. The effects of electron beam (EB) sterilization on BMP‐2 and IGF‐1‐loaded MPs and their biological effects are examined. The synergistic effect of a controlled dual release of BMP‐2 and IGF‐1 on osteogenesis of human mesenchymal stem cells (MSCs) is evaluated. Encapsulation efficiency of growth factors into gelatin and PLGA‐PEG‐COOH MPs is in the range of 64.78% to 76.11%. E‐beam sterilized growth factor delivery systems are effective in significantly promoting osteogenesis of MSCs, although E‐beam sterilization decreases the bioactivity of growth factors in MPs by ≈22%. BMP‐2 release behavior from gelatin MPs/PEG hydrogel shows a faster release (52.7%) than that of IGF‐1 from the PLGA‐PEG‐COOH MPs/PEG hydrogel (27.3%). The results demonstrate that the gelatin and PLGA‐PEG‐COOH MPs‐based delivery system can realize temporal release of therapeutic biomolecules by incorporating different growth factors into distinct microparticles. EB sterilization is an accessible method for sterilizing growth factors‐loaded carriers, which can pave the way for implementing growth factor delivery in clinical applications.

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“…For PLGA materials in particular, many researchers have fabricated PLGA scaffolds for bone defect repair; they show good biocompatibility, low immunogenicity, and toxicity and are widely used in pharmaceutical and tissue engineering as an FDA certificated medical materials. However, PLGA scaffolds have hydrophobic surfaces and unsatisfactory mechanical properties, which have restricted its regenerative stimulation of large bone defects [8]. Therefore, in order to improve the bioactivity of PLGA, various chemical or physical methods have been employed, such as plasma treatment, photoreactive gelatin, poly(dopamine) coating, and nanoparticle doping [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Cell Proliferation and Osteogenesis Differentiation through a Combined Treatment of Poly-L-Lysine-Coated PLGA/Graphene Oxide Hybrid Fiber Matrices and Electrical Stimulation

Zhu

Zheng

et al. 2020

Journal of Nanomaterials

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Bone tissue engineering scaffold provides an effective treatment for bone defect repair. Biodegradable bone scaffold made of various synthetic and natural materials can be used as bone substitutes and grafts for defect site, which has great potential to support bone regeneration. Regulation of cell-scaffold material interactions is an important factor for modulating the cellular activity in bone tissue engineering scaffold applications. Thus, the hydrophilic, mechanical, and chemical properties of scaffold materials directly affect the results of bone regeneration and functional recovery. In this study, a poly-L-lysine (PLL) surface-modified poly(lactic-co-glycolic acid) (PLGA)/graphene oxide (GO) (PLL-PLGA/GO) hybrid fiber matrix was fabricated for bone tissue regeneration. Characterization of the resultant hybrid fiber matrices was done using scanning electron microscopy (SEM), contact angle, and a material testing machine. According to the results obtained from the test above, the PLL-PLGA/GO hybrid fiber matrices exhibited high wettability and mechanical strength. The special surface characteristics of PLL-PLGA/GO hybrid fiber matrices were more beneficial for protein adsorption and inhibit the proliferation of pathogens. Moreover, the enhanced regulation of MC3T3-E1 cell proliferation and differentiation was observed, when the resultant hybrid fiber matrices were combined with electrical stimulation (ES). The cellular response of MC3T3-E1 cells including cell adhesion, proliferation, alkaline phosphatase (ALP) activity, calcium deposition, and osteogenesis-related gene expression was significantly enhanced with the synergistic effect of resultant hybrid fiber matrices and ES. These data indicate that the PLL-PLGA/GO hybrid fiber matrices supported the cellular response in terms of cell proliferation and osteogenesis differentiation in the presence of electrical stimulation, which could be a potential treatment for bone defect.

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Improving osteogenesis of PLGA/HA porous scaffolds based on dual delivery of BMP-2 and IGF-1viaa polydopamine coating

Abstract: To engineer bone tissue, an ideal biodegradable implant should be biocompatible, biodegradable, osteoinductive and osteoconductive.

Cited by 62 publications

References 43 publications

BMP-2-releasing gelatin microspheres/PLGA scaffolds for bone repairment of X-ray-radiated rabbit radius defects

BMP-2-releasing gelatin microspheres/PLGA scaffolds for bone repairment of X-ray-radiated rabbit radius defects

Development of PLGA‐PEG‐COOH and Gelatin‐Based Microparticles Dual Delivery System and E‐Beam Sterilization Effects for Controlled Release of BMP‐2 and IGF‐1

Enhanced Cell Proliferation and Osteogenesis Differentiation through a Combined Treatment of Poly-L-Lysine-Coated PLGA/Graphene Oxide Hybrid Fiber Matrices and Electrical Stimulation

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