Improving of lipid productivity of the oleaginous microalgae Chlorella pyrenoidosa via atmospheric and room temperature plasma (ARTP)

Cao, Song; Zhou, Xu; Jin, Wei; Wang, Feng; Tu, Renjie; Han, Song-Fang; Chen, Hongyi; Chen, Chuan; Xie, Guo-Jun; Ma, Fang

doi:10.1016/j.biortech.2017.05.039

Cited by 112 publications

(48 citation statements)

References 18 publications

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“…More recently, a novel mutagenesis approach, the atmosphere and room temperature plasma (ARTP) mutation, developed by Tsinghua University, is becoming increasingly popular owing to its rapid mutation, highly diverse mutants, as well as simple and safe operation . ARTP has been demonstrated as a powerful tool for phenotype improvement in many different strains (Cao et al 2017b;Chen 2016;Jiang et al 2014). We have applied this method in Z. mobilis to improve its tolerance against low pH and high glucose concentration with positive preliminary results.…”

Section: Discussionmentioning

confidence: 99%

Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

Yang

Tang

et al. 2018

Bioresour. Bioprocess.

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Pretreatment is the key step to overcome the recalcitrance of lignocellulosic biomass making sugars available for subsequent enzymatic hydrolysis and microbial fermentation. During the process of pretreatment and enzymatic hydrolysis as well as fermentation, various toxic compounds may be generated with strong inhibition on cell growth and the metabolic capacity of fermenting strains. Zymomonas mobilis is a natural ethanologenic bacterium with many desirable industrial characteristics, but it can also be severely affected by lignocellulosic hydrolysate inhibitors. In this review, analytical methods to identify and quantify potential inhibitory compounds generated during lignocellulose pretreatment and enzymatic hydrolysis were discussed. The effect of hydrolysate inhibitors on Z. mobilis was also summarized as well as corresponding approaches especially the high-throughput ones for the evaluation. Then the strategies to enhance inhibitor tolerance of Z. mobilis were presented, which include both forward and reverse genetics approaches such as classical and novel mutagenesis approaches, adaptive laboratory evolution, as well as genetic and metabolic engineering. Moreover, this review provided perspectives and guidelines for future developments of robust strains for efficient bioethanol or biochemical production from lignocellulosic materials.

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Section: Discussionmentioning

confidence: 99%

Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

Yang

Tang

et al. 2018

Bioresour. Bioprocess.

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“…An EPS-deficient strain of NX-11 named NX-11 eps− was bred through ARTP mutagenesis according to the method of Cao et al (2017) with some modifications. Briefly, 15 µL of NX-11 culture (1 × 10 7 cells mL −1 ) was coated on a stainless-steel minidisc that was then placed in an ARTP breeding machine (Wuxi TMAXTREE Biotechnology Co., Ltd., Wuxi, China).…”

Section: Acquisition Of An Eps-deficient Strain By Atmospheric and Romentioning

confidence: 99%

The Endophyte Pantoea alhagi NX-11 Alleviates Salt Stress Damage to Rice Seedlings by Secreting Exopolysaccharides

et al. 2020

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Endophytes have the potential to enhance the ability of plants to resist salt stress, improving crop development and yield. Therefore, in this study, we isolated an endophyte that produced large amounts of exopolysaccharides (EPSs) from the roots of sea rice and examined its effects on the physiological responses of rice (Oryza sativa L. ssp. japonica "Nipponbare") seedlings to salt stress using hydroponic experiments. The endophyte was named Pantoea alhagi NX-11 based on its morphological characteristics and 16S ribosomal DNA (rDNA) sequence alignment. Rice seedlings that had been inoculated with P. alhagi NX-11 exhibited a 30.3% increase in fresh weight, a 28.6% increase in root length, a 51.6% increase in shoot length, and a 26.3% increase in chlorophyll content compared with control seedlings under normal conditions. In addition, inoculated rice seedlings had a 37.5% lower malondialdehyde content, a 133% higher K + /Na + ratio, and a 52.8% higher proline content after 7 days under salt stress, as well as up-regulated expression of proline synthase, down-regulated expression of proline dehydrogenase, and enhanced antioxidant enzyme activities. Interestingly, rice seedlings that were inoculated with an EPS-deficient strain named NX-11 eps− that was obtained by atmospheric and room temperature plasma (ARTP) mutagenesis were damaged by salt stress and had similar physiological and biochemical indicators to the control group. Therefore, we speculate that the ability of P. alhagi NX-11 to enhance the salt tolerance of rice seedlings is related to the EPSs it produces.

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“… successfully used ARTP to mutate and evolve Candida glabrata and Yarrowia lipolytica for improved pyruvate and alpha‐ketoglutaric acid production, respectively. In addition, a variety of additional chemical overproduction and tolerance phenotypes have been achieved using ARTP mutagenesis followed by screening and selection including ethanol‐tolerance, lycopene production, enzyme production, cell growth and succinic acid production, butanol production, amino acid production, and lipid productivity . The coupling of these approaches with other selections (including adaptive laboratory evolution) has proven to be a successful method as demonstrated by Ma et al .…”

Section: Chemical and Physical Mutagenesis Of Dnamentioning

confidence: 99%

“…In addition, a variety of additional chemical overproduction and tolerance phenotypes have been achieved using ARTP mutagenesis followed by screening and selection including ethanol-tolerance, 73 lycopene production, 74 enzyme production, 75,76 cell growth and succinic acid production, 77 butanol production, 78 amino acid production, 79 and lipid productivity. 80,81 The coupling of these approaches with other selections (including adaptive laboratory evolution) has proven to be a successful method as demonstrated by Ma et al 77 by coupling ARTP and ALE to improve anaerobic cell growth and succinic acid production in E. coli 3.12-and 2.5-fold, respectively.…”

Section: Radiation-induced Mutagenesismentioning

confidence: 99%

Strategies for directed and adapted evolution as part of microbial strain engineering

Zhou

Alper

2018

J of Chemical Tech & Biotech

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The industrial production of chemicals by microorganisms usually requires improvements to the enzymes, pathways, and strain that go beyond the capacity of innate enzymes. To achieve these phenotypes and overcome our limited capacity to de novo design these parts, directed and adaptive evolutionary approaches are used to explore new functions. This review highlights the recent advances in both sequence diversity generation and selection strategies from traditional in vitro mutagenesis to novel in vivo continuous evolution applications. The focus here is on comparison of the different gene diversity methods in an attempt to distinguish the best strategy for protein or strain engineering for a given goal. Furthermore, the important role that screening and selection can play in advancing directed and adapted evolution is discussed. © 2018 Society of Chemical Industry

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Improving of lipid productivity of the oleaginous microalgae Chlorella pyrenoidosa via atmospheric and room temperature plasma (ARTP)

Cited by 112 publications

References 18 publications

Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

The Endophyte Pantoea alhagi NX-11 Alleviates Salt Stress Damage to Rice Seedlings by Secreting Exopolysaccharides

Strategies for directed and adapted evolution as part of microbial strain engineering

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