Improving micropropagation conditions for adult-phase shoots of chestnut

Sánchez, Conchi; San-Jose, M. Carmen; Ferro, Enrique; Ballester, A.; Viéitez, A. M.

doi:10.1080/14620316.1997.11515531

Cited by 32 publications

(8 citation statements)

References 3 publications

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“…The similar rooting responses of microshoots initiated from these trees suggest a close genotypic relationship, at least for the rooting competence trait. By contrast, the rooting responses of several clones of chestnut reported by Sánchez et al (1997b) were lower than obtained in clones P1 and P2 for the same treatment. These differences are probably a consequence of the genotype effect on the rooting ability (Bonga 1987).…”

Section: Discussioncontrasting

confidence: 54%

“…In addition to being affected by auxin, the maturation stage and other factors that regulate rooting, AR competence is a quantitative trait (Riemenschneider 1993) and is thus highly genotype-dependent. Genotypic differences in the rooting ability as well as in the rooting response of shoots to a particular inductive treatment have been reported in chestnut and oak (Dantas et al 2016;Osterc et al 2006;Sánchez et al 1996;Sánchez et al 1997b).…”

Section: Introductionmentioning

confidence: 97%

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Effects of auxin and urea derivatives on adventitious rooting in chestnut and oak microshoots

Vielba

Vidal

Ricci

et al. 2020

Israel J. Plant Sci.

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The present study investigated how auxin concentration and the method of application affected the formation of adventitious roots in microshoots of chestnut (Castanea sativa) and oak (Quercus robur). The activity of two urea derivatives (2, 3-MDPU and 3, 4-MDPU) was also evaluated. Microshoots were derived from basal sprouts of two mature chestnut trees (P1 and P2) and one adult oak genotype (Sainza). In chestnut, rooting percentage was positively affected by auxin in a dose-dependent manner, particularly in shoots treated with the hormone for 24 h. The effect of auxin on rooting also differed depending on the application method. In shoots treated for 24 h, the highest concentration of auxin produced the healthiest rooted plantlets, in terms of the root system and shoot quality. By contrast, in shoots treated by the basal quick-dip method, the shoot quality was best at the lowest auxin concentration. The effect of urea derivatives on the root system depended on the species as well as on the auxin concentration and application period. Use of the MDPUs improved the root system architecture of auxin-treated shoots by promoting lateral root development and triggering the synchronous initiation of root primordia at the base of the shoot. Shoot quality was also improved by MDPUs, which promoted resumption of growth and reduced shoot-tip necrosis.

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Section: Discussioncontrasting

confidence: 54%

Section: Introductionmentioning

confidence: 97%

Effects of auxin and urea derivatives on adventitious rooting in chestnut and oak microshoots

Vielba

Vidal

Ricci

et al. 2020

Israel J. Plant Sci.

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“…However, in relation to leaf parameters, only basal explants of clones C063 and P042 had significantly longer leaves, and only the latter had significantly wider leaves. The positive effect of using basal explants with the attached callus has already been discussed in studies involving chestnut micropropagation in gelled medium (Sánchez et al 1997). These authors attributed the greater number and length of shoots formed by basal explants to two reasons.…”

Section: Explant Type and Sizementioning

confidence: 90%

Micropropagation of axillary shoots of hybrid chestnut (Castanea sativa × C. crenata) in liquid medium in a continuous immersion system

Cuenca

Sánchez

Aldrey

et al. 2017

Plant Cell Tiss Organ Cult

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A protocol for culturing chestnut axillary shoots with the basal sections continuously immersed in liquid medium was developed and the influence of various parameters was assessed: explant type and size, support material, bioreactor size and aeration regime. Shoots excised from eight selected chestnut genotypes were successfully cultured in 10 L bioreactors in liquid Murashige and Skoog medium with half strength nitrates and supplemented with 0.05 mg/L N 6-benzyladenine and 30 g/L sucrose. Forced ventilation and the use of a support material were essential for producing healthy, non-hyperhydric shoots. High proliferation rates were achieved and good quality shoots ready for multiplication, rooting and acclimatization were obtained. Larger leaves showing higher levels of photosynthetic pigments were observed in shoots cultured in bioreactors, suggesting a certain degree of photoautotrophy in shoots cultured under forced ventilation. This is the first demonstration of the production of chestnut shoots in stationary liquid medium, indicating the feasibility of the method for large-scale propagation of the species.

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“…Efforts to overcome chestnut recalcitrance to rooting have created a body of knowledge describing a vast range of micropropagation techniques [13,16,20,[32][33][34]. Gonçalves et al tested MS semisolid media with concentrations of IBA from 1.5 to 5 mg/L in chestnut hybrids [16].…”

Section: Discussionmentioning

confidence: 99%

A New Clonal Propagation Protocol Develops Quality Root Systems in Chestnut

Fernandes

Tedesco

Silva

et al. 2020

Forests

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There is, at the present time, a great demand for chestnut rootstocks with improved resistance to Phytophthora cinnamomi Rands in the nurseries. New genotypes are emerging from European chestnut breeding programs and the production of thriving plants to restore old orchards with low yields due to a high incidence of diseases, namely root rot, is necessary. Micropropagation is a useful technique for clonal propagation. Nevertheless, in vitro culture propagation is genotype-dependent. Consequently, the existing protocols may demonstrate poor reproducibility and low efficacy. Thus, the need to contribute to the development of new micropropagation protocols suitable for large production of emerging genotypes. As a contribution to fill this gap, a three-step protocol was developed by using new combinations of Murashige & Skoog, Woody Plant, and adapted modified Melin-Norkrans media in different stages of the propagation process. About 90% of shoots were rooted, and after three months of acclimatization, 85% of these plants survived and were capable of continuous growth in the field. Currently, this protocol is being used in the production of several hybrid genotypes (with improved resistance to P. cinnamomi), selected from our ongoing breeding program and also in Castanea sativa Mill. and Castanea crenata Siebold and Zucc. species.

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Improving micropropagation conditions for adult-phase shoots of chestnut

Cited by 32 publications

References 3 publications

Effects of auxin and urea derivatives on adventitious rooting in chestnut and oak microshoots

Effects of auxin and urea derivatives on adventitious rooting in chestnut and oak microshoots

Micropropagation of axillary shoots of hybrid chestnut (Castanea sativa × C. crenata) in liquid medium in a continuous immersion system

A New Clonal Propagation Protocol Develops Quality Root Systems in Chestnut

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