Improving microbial oil production with standard and native oleaginous yeasts by using Taguchi design

The capability of 17 Rhodotorula spp. isolated from Antarctica to accumulate intracellular lipids in nitrogen-limited medium was investigated. As results, 10 isolates were selected by Nile red staining, while 12 isolates were selected as oleaginous by analysis of total lipid content (20.4-73%, w/w of dry biomass). The higher lipid production and accumulation was exhibited for six strains belonging to three species of Rhodotorula (Rhodotorula glutinis, Rhodotorula glacialis, and Rhodotorula laryngis). This is the first report where R. laryngis have been identified within oleaginous specie. Lipid accumulation was evaluated comparatively in two nitrogen-limited glucose-based media (MI and MII). MI (low C/N ratio) was more suitable for biomass and lipid production while in MII (high C/N ratio) total lipid content was improved. R. glutinis R4, R. glacialis R15, and R. glutinis R48 showed high lipid concentrations (4.65-6.93 g L ) and they were able to accumulate large amounts of lipids per gram of biomass (47-77%, w/w). A similar profile in fatty acids composition and content of neutral lipids to vegetable oils was observed, indicating that lipids produced by oleaginous Antarctic yeasts can be considered an alternative feedstock for biodiesel production. Antarctica represents an important source of oleaginous yeasts with adaptive capabilities to accumulate considerable amounts of lipids with biotechnological interest at 15 °C and 25 °C.

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“…In addition, fluorescence emission observed in Fig. was similar to those described in several oleaginous yeasts .…”

Section: Discussionsupporting

confidence: 84%

“…The high oleic and linoleic acids content observed here, and common for yeasts, is considered favorable for biodiesel applications . Furthermore, it has been concluded that yeast oils would be appropriate for use as biodiesel at a similar blending ratio as biodiesel originating from vegetable oils .…”

Section: Discussionmentioning

confidence: 64%

Oleaginous yeasts from Antarctica: Screening and preliminary approach on lipid accumulation

Viñarta

Angelicola

Barros

et al. 2016

J. Basic Microbiol.

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“…The pH was measured by a laboratory pH meter (METTLER TOLEDO FiveEasy, Switzerland). The concentration of glucose was determined by the 3,5-dinitrosalicylic acid (DNS) method [44,45]. NH 4 + concentration was measured by the indophenol blue spectrophotometric method [46].…”

Section: Determination Of Dry Cell Weight Ph and Glucose And Nitrogementioning

confidence: 99%

Enhancement of docosahexaenoic acid production by overexpression of ATP-citrate lyase and acetyl-CoA carboxylase in Schizochytrium sp.

Xiao

Zhao

Wen

et al. 2020

Biotechnol Biofuels

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Background: Docosahexaenoic acid (DHA) is an important omega-3 long-chain polyunsaturated fatty acid that has a variety of physiological functions for infant development and human health. Although metabolic engineering was previously demonstrated to be a highly efficient way to rapidly increase lipid production, metabolic engineering has seldom been previously used to increase DHA accumulation in Schizochytrium spp. Results: Here, a sensitive β-galactosidase reporter system was established to screen for strong promoters in Schizochytrium sp. Four constitutive promoters (EF-1α p , TEF-1 p , ccg1 p , and ubiquitin p) and one methanol-induced AOX1 promoter were characterized by the reporter system with the promoter activity ccg1 p > TEF-1 p > AOX1 p (induced) > EF-1α p > ubiquitin p. With the strong constitutive promoter ccg1 p , Schizochytrium ATP-citrate lyase (ACL) and acetyl-CoA carboxylase (ACC) were overexpressed in Schizochytrium sp. ATCC 20888. The cells were cultivated at 28 °C and 250 rpm for 120 h with glucose as the carbon source. Shake-flask fermentation results showed that the overexpression strains exhibited growth curves and biomass similar to those of the wild-type strain. The lipid contents of the wild-type strain and of the OACL, OACC, and OACL-ACC strains were 53.8, 68.8, 69.8, and 73.0%, respectively, and the lipid yields of the overexpression strains were increased by 21.9, 30.5, and 38.3%, respectively. DHA yields of the wild-type strain and of the corresponding overexpression strains were 4.3, 5.3, 6.1, and 6.4 g/L, i.e., DHA yields of the overexpression strains were increased by 23.3, 41.9, and 48.8%, respectively. Conclusions: Acetyl-CoA and malonyl-CoA are precursors for fatty acid synthesis. ACL catalyzes the conversion of citrate in the cytoplasm into acetyl-CoA, and ACC catalyzes the synthesis of malonyl-CoA from acetyl-CoA. The results demonstrate that overexpression of ACL and ACC enhances lipid accumulation and DHA production in Schizochytrium sp.

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“…Glucose has been reported as the best substrate for microbial oil production, since it can be assimilated by most oleaginous microorganisms to produce oil [ 18 ]. Therefore, equivalent glucose contents from each collected waste were determined as described by Saeman et al [ 10 ] and Miller [ 11 ].…”

Section: Resultsmentioning

confidence: 99%

Utilization of Solid Waste as a Substrate for Production of Oil from Oleaginous Microorganisms

et al. 2018

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The overwhelming demand of oil and fats to meet the ever increasing needs for biofuel, cosmetics production, and other industrial purposes has enhanced a number of innovations in this industry. One such innovation is the use of microorganisms as alternative sources of oil and fats. Organic solid waste that is causing a big challenge of disposal worldwide is biodegradable and can be utilized as substrate for alternative oil production. The study evaluated the potential of isolated yeast-like colonies to grow and accumulate oil by using organic solid waste as substrate. Of the 25 yeast-like colonies isolated from the soil samples collected from three different suburbs in Kampala district, Uganda, 20 were screened positive for accumulation of lipid but only 2 were oleaginous. The NHC isolate with the best oil accumulation potential of 48.8% was used in the central composite design (CCD) experiments. The CCD experimental results revealed a maximum oil yield of 61.5% from 1.25 g/L cell biomass at 10 g/L of solid waste and temperature of 25°C. The study revealed that organic solid waste could be used as a substrate for microbial oil production.

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Improving microbial oil production with standard and native oleaginous yeasts by using Taguchi design

Abstract: Production of microbial oil has attracted a great attention

Cited by 30 publications

References 24 publications

Oleaginous yeasts from Antarctica: Screening and preliminary approach on lipid accumulation

Oleaginous yeasts from Antarctica: Screening and preliminary approach on lipid accumulation

Enhancement of docosahexaenoic acid production by overexpression of ATP-citrate lyase and acetyl-CoA carboxylase in Schizochytrium sp.

Utilization of Solid Waste as a Substrate for Production of Oil from Oleaginous Microorganisms

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