Improving HEK293-based AAV-production using GSMMs, and a multi-omics approach
L. Zehetner,
D. Széliová,
B. Kraus
et al.
Abstract:HEK293 cells are a versatile cell line extensively used in the production of recombinant proteins and viral vectors, notably Adeno-associated virus (AAV) [12]. Despite their high transfection efficiency and adaptability to various culture conditions, challenges remain in achieving sufficient yields of active viral particles. This study presents a comprehensive multi-omics analysis of two HEK293 strains under good manufacturing practice conditions, focusing on the metabolic and cellular responses during AAV pro… Show more
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