Improving evaluation of potato resistance to Rhizoctonia solani infection by optimizing inoculum-based method combined with toxin-based assay

Zhang, Xiaoyu; Li, Dezhou; Huo, H. L.; Xing, Xing; Lian, Yong; Zhuo, Yu; Hao, Jianjun

doi:10.1016/j.cropro.2021.105544

Cited by 10 publications

(8 citation statements)

References 33 publications

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“…Previous reports have shown that CAT and PG play critical roles in the pathogenicity of R. solani [ 44 , 45 , 48 , 49 ]. In order to determine whether exogenous sprays of dsRNAs targeting RsPG1 , RsCAT1, and RsCRZ1 can reduce the virulence of R. solani and its destructive effects on host crops, we evaluated the effects of the SIGS technology on controlling R. solani infection using dsRNAs specific to these genes.…”

Section: Introductionmentioning

confidence: 99%

Nanosheet-Facilitated Spray Delivery of dsRNAs Represents a Potential Tool to Control Rhizoctonia solani Infection

Chen

Shi

Tang

et al. 2022

IJMS

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Rhizoctonia solani is one of the important pathogenic fungi causing several serious crop diseases, such as maize and rice sheath blight. Current methods used to control the disease mainly depend on spraying fungicides because there is no immunity or high resistance available in crops. Spraying double-strand RNA (dsRNA) for induced-gene silencing (SIGS) is a new potentially sustainable and environmentally friendly tool to control plant diseases. Here, we found that fluorescein-labelled EGFP-dsRNA could be absorbed by R. solani in co-incubation. Furthermore, three dsRNAs, each targeting one of pathogenicity-related genes, RsPG1, RsCATA, and RsCRZ1, significantly downregulated the transcript levels of the target genes after co-incubation, leading to a significant reduction in the pathogenicity of the fungus. Only the spray of RsCRZ1 dsRNA, but not RsPG1 or RsCATA dsRNA, affected fungal sclerotium formation. dsRNA stability on leaf surfaces and its efficiency in entering leaf cells were significantly improved when dsRNAs were loaded on layered double hydroxide (LDH) nanosheets. Notably, the RsCRZ1-dsRNA-LDH approach showed stronger and more lasting effects than using RsCRZ1-dsRNA alone in controlling pathogen development. Together, this study provides a new potential method to control crop diseases caused by R. solani.

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Section: Introductionmentioning

confidence: 99%

Nanosheet-Facilitated Spray Delivery of dsRNAs Represents a Potential Tool to Control Rhizoctonia solani Infection

Chen

Shi

Tang

et al. 2022

IJMS

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“…Two disease complexes, stem canker and black scurf, are economically significant because they devastate the quality and quantity of potato crops all over the world [ 17 ]. Rhizoctonia causes an annual potato production loss ranging from 7% to 36% worldwide, with Western Siberia contributing to more than 50% of the total crop yield loss [ 18 ]. R. solani is hard to control because it is soil-borne in nature, and the fungus’s diverse host range adds to the difficulty [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Unveiling the Genetic Tapestry: Exploring Rhizoctonia solani AG-3 Anastomosis Groups in Potato Crops across Borders

Naqvi,

Abbas,

Farhan

et al. 2024

Plants

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The current study was carried out to screen 10 isolates (ARS-01–ARS-10) of Rhizoctonia. solani from potato tubers cv. Kuroda, which were collected from various potato fields in Multan, Pakistan. The isolates were found to be morphologically identical, as the hyphae exhibit the production of branches at right angles and acute angles often accompanied by septum near the emerging branches. Anastomosis grouping showed that these isolates belonged to AG-3. A pathogenicity test was performed against the susceptible Kuroda variety and among the isolates, ARS-05 exhibited the highest mean severity score of approximately 5.43, followed by ARS-09, which showed a mean severity score of about 3.67, indicating a moderate level of severity. On the lower end of the severity scale, isolates ARS-06 and ARS-07 displayed mean severity scores of approximately 0.53 and 0.57, respectively, suggesting minimal symptom severity. These mean severity scores offer insights into the varying degrees of symptom expression among the different isolates of R. solani under examination. PCoA indicates that the severe isolate causing black scurf on the Kuroda variety was AG-3. A comprehensive analysis of the distribution, genetic variability, and phylogenetic relationships of R. solani anastomosis groups (AGs) related to potato crops across diverse geographic regions was also performed to examine AG prevalence in various countries. AG-3 was identified as the most widespread group, prevalent in Sweden, China, and the USA. AG-5 showed prominence in Sweden and the USA, while AG-2-1 exhibited prevalence in China and Japan. The phylogenetic analysis unveiled two different clades: Clade I comprising AG-3 and Clade II encompassing AG-2, AG-4, and AG-5, further subdivided into three subclades. Although AGs clustered together regardless of origin, their genetic diversity revealed complex evolutionary patterns. The findings pave the way for region-specific disease management strategies to combat R. solani’s impact on potato crops.

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“…The absence of resistant varieties may result in a mortality of approximately 50% of seedlings before emergence, with "white leg" symptoms appearing on nearly all plants during the growing season and sclerotia formation on the majority of tubers. Under favourable conditions, disease not only compromises seed material quality, but also causes substantial losses, potentially reaching 49% of the harvest (Zhang et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Field Evaluation of Ukrainian Potato Varieties for Resistance to Fungal and Bacterial Pathogens in the Polissya Area of Ukraine

Taktaev,

Podberezko,

Janse

2024

Research in Agricultural Sciences

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This study aimed to assess the resistance of Ukrainian potato varieties to fungal and bacterial pathogens under natural infectious conditions in the Polissya zone of Ukraine. Field trials were conducted during 2019-2022 to examine the manifestation and spread of fungal and bacterial diseases on 20 Ukrainian potato varieties across different maturity groups. Varieties were evaluated for resistance to Alternaria blight, Rhizoctonia, common scab, and Fusarium dry rot using predefined scales. The Ukrainian potato varieties Shchedryk, Aria, Slovianka, Myroslava, and Khortytsia displayed high resistance to Alternaria. The varieties Shchedryk, Okolytsia, and Feia showed resistance to Rhizoctonia. The varieties Skarbnytsia, Slovianka, and Okolytsia were highly resistant to common scab, while Kimmeria, Shchedryk, Tyras, Aria, Slovianka, and Letana exhibited resistance to Fusarium dry rot. Several potato varieties displayed field resistance to multiple pathogens. On the base of the results, the potato varieties such as Feia, Khortytsia, Kimmeria, Tyras, Aria, Slovianka, Anika, and Shchedryk, with resistance to 3-4 pathogens, hold promise for integration into cropping systems with reduced fungicide usage. Additionally, these varieties can be recommended for inclusion in breeding programs as valuable sources of resistance to these fungal and bacterial pathogens. Future research should focus on elucidating the genetic basis of resistance in these varieties and further exploring the nature of inheritance from the parental forms, including Bellarosa, Beloruskyi 3, Bahriana, Slovianka, Oberih, Lyu, Meve, Kondor, Tyras, Barylchykha, and hybrids 86.281c12, KE 78.50.53, 77.583/16, and P.88.12-11.

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Improving evaluation of potato resistance to Rhizoctonia solani infection by optimizing inoculum-based method combined with toxin-based assay

Cited by 10 publications

References 33 publications

Nanosheet-Facilitated Spray Delivery of dsRNAs Represents a Potential Tool to Control Rhizoctonia solani Infection

Nanosheet-Facilitated Spray Delivery of dsRNAs Represents a Potential Tool to Control Rhizoctonia solani Infection

Unveiling the Genetic Tapestry: Exploring Rhizoctonia solani AG-3 Anastomosis Groups in Potato Crops across Borders

Field Evaluation of Ukrainian Potato Varieties for Resistance to Fungal and Bacterial Pathogens in the Polissya Area of Ukraine

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