Improving electroautotrophic ammonium production from nitrogen gas by simultaneous carbon dioxide fixation in a dual−chamber microbial electrolysis cell

Zhang, Lixia; Tian, Changlin; Wang, Haoran; Gu, Wenzhi; Zheng, Dinghai; Cui, Mengyao; Wang, Xiaomei; He, Xiaohong; Zhan, Guoqiang; Li, Daping

doi:10.1016/j.bioelechem.2021.108044

Cited by 19 publications

(19 citation statements)

References 47 publications

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“…The copyright holder for this preprint this version posted March 7, 2023. ; https://doi.org/10.1101/2023.03.02.530809 doi: bioRxiv preprint the current densities observed here (-5 to -10 A/m²) were about 1000 times higher. These current density levels are close to those measured by Zhang et al (L. Zhang et al, 2022) who reported a maximum of -10 A/m² at the same applied potential.…”

Section: Resultssupporting

confidence: 89%

Section: Resultssupporting

confidence: 60%

“…During the first 214 days of enrichment, 0.6 to 3.3% of the electrons were used for N 2 fixation in the two PCE. In comparison, efficiencies of 0.5% and 20% for NH 4 + synthesis was reported in two recent works carried out under similar conditions (Yadav et al, 2022; L. Zhang et al, 2022). As the amount of fixed N was highly dependent on biomass accumulation, negative results were obtained at day 244 when biomass started to decrease.…”

Section: Resultsmentioning

confidence: 52%

“…The fixed N is then used for the synthesis of proteins and in particular for the synthesis of L-glutamate and L-glutamine which are inhibitors of nitrogenase synthesis (Moreno-Vivián et al, 1989; Y. Zhang et al, 2022). Interestingly, nitrogenases are not fully specific and are able to catalyze other reactions such as the production of H 2 and the reduction of ethylene to acetylene which require less energy (Burgess & Lowe, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Rous

Santa-Catalina

Quéméner

et al. 2023

Preprint

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The production of nitrogen fertilizers in modern agriculture is mostly based on the Haber-Bosch process, representing nearly 2% of the total energy consumed in the world. Low-energy bioelectrochemical fixation of N2to microbial biomass was previously observed on a biocathode but the microbial interactions in N2-fixing electroactive biofilms are still poorly understood. The present study aims to develop a method of enrichment of autotrophic and diazotrophic bacteria from soil samples for further characterization. The enrichment method was based on a first classical step of selection of N2-fixing bacteria from soil samples. Then, a polarized cathode was used for the enrichment of autotrophic bacteria using H2(hydrogenotrophic) or the cathode as energy sources. This enrichment was compared with an enrichment of diazotrophic hydrogenotrophic bacteria without the use of the microbial electrochemical system. Both methods showed comparable results for N2fixation rates at day 340 of the enrichment, with an estimated average of approximately 0.2 mgNfixed/L.d. Current densities up to -15 A/m² were observed in the polarized cathode enrichments and a significant increase of the microbial biomass on the cathode was shown between 132 and 214 days of enrichment. These results confirm the enrichment of autotrophic, electrotrophic and diazotrophic bacteria in the polarized cathode enrichments. Finally, the analysis of the enriched communities suggest thatDesulforamulus ruminismediated microbial interactions between autotrophic anaerobic and heterotrophic aerobic bacteria in polarized cathode enrichment. These interactions could play a key role in the development of biomass in these systems and on N2fixation. Based on these findings, a conceptual model on the functioning of mixed cultures N2-fixing electroactive biofilms was proposed.

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Section: Resultssupporting

confidence: 89%

Section: Resultssupporting

confidence: 60%

Section: Resultsmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

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Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Rous

Santa-Catalina

Quéméner

et al. 2023

Preprint

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“…( 1)) (Cai et al, 2018;Zheng et al, 2020). Cathode bacteria can also consume the organic reduction products (VFAs) from bicarbonate as a carbon source (Zhang et al, 2022).…”

Section: Effect Of Nitrate On the Fe(ii) Regeneration And Bicarbonate...mentioning

confidence: 99%

Improved Fe(II) regeneration from actual ferric sludge using a biocathode with granular sludge

Wang

Tang

Yao

et al. 2023

Journal of Cleaner Production

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Enhanced Microbial Protein Production from CO₂ and Air by a MoS₂ Catalyzed Bioelectrochemical System

Zhang,

Zeng,

Wang

et al. 2024

ChemPlusChem

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Carbon dioxide can be relatively easily reduced to organic compounds in a bioelectrochemical system (BES). However, due to insufficient reduction force from in‐situ hydrogen evolution, it is difficult for nitrogen reduction. In this study, MoS2 was firstly used as an electrocatalyst for the simultaneous reduction of CO2 and N2 to produce microbial protein (MP) in a BES. Cell dry weight (CDW) could reach 0.81±0.04 g/L after 14 d operation at −0.7 V (vs. RHE), which was 108±3% higher than that from non−catalyst control group (0.39±0.01 g/L). The produced protein had a better amino acid profile in the BES than that in a direct hydrogen system (DHS), particularly for proline (Pro). Besides, MoS2 promoted the growth of bacterial cell on an electrode and improved the biofilm extracellular electron transfer (EET) by microscopic observation and electrochemical characterization of MoS2 biocathode. The composition of the microbial community and the relative abundance of functional enzymes revealed that MoS2 as an electrocatalyst was beneficial for enriching Xanthobacter and enhancing CO2 and N2 reduction by electrical energy. These results demonstrated that an efficient strategy to improve MP production of BES is to use MoS2 as an electrocatalyst to shift amino acid profile and microbial community.

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Improving electroautotrophic ammonium production from nitrogen gas by simultaneous carbon dioxide fixation in a dual−chamber microbial electrolysis cell

Cited by 19 publications

References 47 publications

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Improved Fe(II) regeneration from actual ferric sludge using a biocathode with granular sludge

Enhanced Microbial Protein Production from CO₂ and Air by a MoS₂ Catalyzed Bioelectrochemical System

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Improving electroautotrophic ammonium production from nitrogen gas by simultaneous carbon dioxide fixation in a dual−chamber microbial electrolysis cell

Cited by 19 publications

References 47 publications

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Improved Fe(II) regeneration from actual ferric sludge using a biocathode with granular sludge

Enhanced Microbial Protein Production from CO2 and Air by a MoS2 Catalyzed Bioelectrochemical System

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Product

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Enhanced Microbial Protein Production from CO₂ and Air by a MoS₂ Catalyzed Bioelectrochemical System