Caring for preterm infants in the intensive care unit can lead to a variety of conditions that increase stress and pain during routine care procedures. One of the intensive care unit procedures is diapers usage and change. The immature anatomical and physiological growth and development, especially the musculoskeletal system, neurological system, and integumentary system, is a challenge in the care of premature infants. Inappropriate diapers in premature infants can result in neurodevelopment problems, diaper dermatitis, and long-term postural disorders. Supportive care in the intensive care unit should include maintaining sleep cycles, creating a comfortable environment, periodic stress and pain measurement, practicing infants’ skincare, and care involving parents.