Improving blood-compatibility of titanium by coating collagen–heparin multilayers

Chen, J.L.; Li, Q.L.; Chen, J.Y.; Chen, C.; Huang, Nan

doi:10.1016/j.apsusc.2009.03.011

Cited by 107 publications

(93 citation statements)

References 34 publications

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“…We carried out the platelet adhesion experiment to evaluate the surface thrombogenicity of the samples and to examine the interaction between blood and the materials in vitro [20]. Platelet rich plasma (PRP) was prepared by centrifuging 45 ml of fresh whole blood from a porcine donor with 5 ml of a 3.8 wt% sodium citrate solution at 1509g for 15 min and 5009g for 20 min.…”

Section: Platelet Adhesion Testmentioning

confidence: 99%

Thromboresistant and endothelialization effects of dopamine-mediated heparin coating on a stent material surface

Bae

Park

et al. 2012

J Mater Sci: Mater Med

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Heparinization of surfaces has proven a successful strategy to prevent thrombus formation. Inspired by the composition of adhesive proteins in mussels, the authors used dopamine to immobilize heparin on a stent surface. This study aimed to assess the thromboresistant and endothelialization effects of dopamine-mediated heparin (HPM) coating on a stent material surface. The HPM was synthesized by bonding dopamine and heparin chemically. Cobalt-chromium (Co-Cr) alloy disks were first placed in the HPM solution and applied to surface stability then underwent thromboresistant tests and human umbilical vein endothelial cells (HUVEC) cytotoxicity assays. The results showed not only thromboresistant activity and a stable state of heparin on the surfaces after investigation with toluidine blue and thrombin activation assay but also proliferation of HUVEC in vitro. Studies on animals showed that the HPM-coated stent has no obvious inflammation response and increasing of restenosis rate compared to the bare metal stent (BMS) indicating good biocompatibility as well as safety in its in vivo application. Moreover, improving the endothelial cell (EC) proliferation resulted in a higher strut-covering rate (i.e., endothelialization) with shuttle-shaped EC in the HPM-coated stent group compared to that of the BMS group. These results suggest that this facile coating approach could significantly promote endothelialization and offer greater safety than the BMS for its much improved thromboresistant property. Moreover, it may offer a platform for conjugating secondary drugs such as anti-proliferative drugs.

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Section: Platelet Adhesion Testmentioning

confidence: 99%

Thromboresistant and endothelialization effects of dopamine-mediated heparin coating on a stent material surface

Bae

Park

et al. 2012

J Mater Sci: Mater Med

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“…Several approaches to improve their blood compatibility, including coating the device materials with biocompatible films (DLC [1], Ti-O [2], polymers [3], etc.) and immobilizing biomolecules (heparin [4][5][6], chitosan [7], tropoelastin [8], etc.) on them have been explored.…”

Section: Introductionmentioning

confidence: 99%

Coimmobilization of heparin/fibronectin mixture on titanium surfaces and their blood compatibility

Zhang

Liao

et al. 2010

Colloids and Surfaces B: Biointerfaces

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“…Since reported by Decher et al [16], the LBL technique has been proved to be a highly effective method to immobilize multi-components of the ECM on biomaterials [17][18][19][20]. The mechanism of the LBL technique involves two kinds of oppositely charged polyelectrolytes that are alternately absorbed on the material surface by electrostatic interaction, and finally form a polyelectrolyte multilayer (PEM) film [20].…”

Section: Introductionmentioning

confidence: 99%

“…Further details on the LBL techniques are given by Chen et al [17]. TC-A samples were dipped into the collagen solution for 30 min, rinsed with deionized water and then soaked into the HA solution for 30 min, followed by rinsing with deionized water.…”

mentioning

confidence: 99%

Fabrication and in vitro evaluation of stable collagen/hyaluronic acid biomimetic multilayer on titanium coatings

Xie

Tan

et al. 2013

J. R. Soc. Interface.

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Layer-by-layer (LBL) self-assembly technique has been proved to be a highly effective method to immobilize the main components of the extracellular matrix such as collagen and hyaluronic acid on titanium-based implants and form a polyelectrolyte multilayer (PEM) film by electrostatic interaction. However, the formed PEM film is unstable in the physiological environment and affects the long-time effectiveness of PEM film. In this study, a modified LBL technology has been developed to fabricate a stable collagen/hyaluronic acid (Col/HA) PEM film on titanium coating (TC) by introducing covalent immobilization. Scanning electron microscopy, diffuse reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy were used to characterize the PEM film. Results of Sirius red staining demonstrated that the chemical stability of PEM film was greatly improved by covalent crosslinking. Cell culture assays further illustrated that the functions of human mesenchymal stem cells, such as attachment, spreading, proliferation and differentiation, were obviously enhanced by the covalently immobilized Col/ HA PEM on TCs compared with the absorbed Col/HA PEM. The improved stability and biological properties of the Col/HA PEM covalently immobilized TC may be beneficial to the early osseointegration of the implants.

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Improving blood-compatibility of titanium by coating collagen–heparin multilayers

Cited by 107 publications

References 34 publications

Thromboresistant and endothelialization effects of dopamine-mediated heparin coating on a stent material surface

Thromboresistant and endothelialization effects of dopamine-mediated heparin coating on a stent material surface

Coimmobilization of heparin/fibronectin mixture on titanium surfaces and their blood compatibility

Fabrication and in vitro evaluation of stable collagen/hyaluronic acid biomimetic multilayer on titanium coatings

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