Improving biosynthesis of Au Pd core-shell nanoparticles through Escherichia coli with the assistance of phytochelatin for catalytic enhanced chemiluminescence and benzyl alcohol oxidation

Zhang, Dingkun; Tang, Dewei; Yamamoto, Toshiyoshi; Kato, Yuriko; Horiuchi, Shiho; Ogawa, Shinya; Yoshimura, Esturo; Suzuki, Michio

doi:10.1016/j.jinorgbio.2019.110795

Cited by 16 publications

(13 citation statements)

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“…Phytochelatins, the enzymatic products of phytochelatin synthase, are small soluble peptides that play an essential role in resisting heavy metal toxicity in plants [8][9][10][11]. Their potential role in nanoparticle synthesis has been reported [13][14][15][16]. In the present study, we proposed that the overexpression of the enzyme promoted the synthesis of phytochelatins in the host E. coli cells based on literature review and our own observations, although we didn't measure the phytochelatin synthase activity or the level of phytochelatins in the cells.…”

Section: Discussionmentioning

confidence: 78%

“…The thiols of cysteine residues in phytochelatins interact with metals with high affinity and form the phytochelatin-metal complex during detoxification of metals and metalloids in plants [4,8,11]. The contribution of phytochelatins to nanoparticle synthesis has been investigated [13][14][15]. We also developed a recombinant E. coli bacterium that expresses R. tropici phytochelatin synthase, and discovered that overexpression of R. tropici phytochelatin synthase in E. coli cells improved the bacterial heavy metal resistance, and increased yield of selenium nanoparticles when whole cells were used to synthesize nanoparticles [16].…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Extracellular Synthesis of Gold Nanoparticles by Soluble Extracts from Escherichia coli Transformed with Rhizobium tropici Phytochelatin Synthase Gene

Yuan

Bomma

2021

Metals

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Phytochelatins, the enzymatic products of phytochelatin synthase, play a principal role in protecting the plants from heavy metal and metalloid toxicity due to their ability to scavenge metal ions. In the present study, we investigated the capacity of soluble intracellular extracts from E. coli cells expressing R. tropici phytochelatin synthase to synthesize gold nanoparticle. We discovered that the reaction mediated by soluble extracts from the recombinant E. coli cells had a higher yield of gold nanoparticles, compared to that from the control cells. The compositional and morphological properties of the gold nanoparticles synthesized by the intracellular extracts from recombinant cells and control cells were similar. In addition, this extracellular nanoparticle synthesis method produced purer gold nanoparticles, avoiding the isolation of nanoparticles from cellular debris when whole cells are used to synthesize nanoparticles. Our results suggested that phytochelatins can improve the efficiency of gold nanoparticle synthesis mediated by bacterial soluble intracellular extracts, and the potential of extracellular nanoparticle synthesis platform for the production of nanoparticles in large quantity and pure form is worth further investigation.

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Section: Discussionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Enhanced Extracellular Synthesis of Gold Nanoparticles by Soluble Extracts from Escherichia coli Transformed with Rhizobium tropici Phytochelatin Synthase Gene

Yuan

Bomma

2021

Metals

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Section: Purification Of Nps Synthesized By Microorganismsmentioning

confidence: 93%

“…In the BAO test, a mixture of H 2 O 2 , cetrimonium bromide, and Au-Pd NPs generated benzyl alcohol, benzaldehyde, and benzoic acid, which was detected using gas chromatography and mass spectrometry. These tests demonstrated that the Au-Pd NPs were catalytically active[80].The cysteine desulfhydrase gene of Treponema denticola was over-expressed in E. coli to synthesize CdS NPs. Incubation of the cells in a culture medium supplemented with cadmium ions and cysteine resulted in CdS NPs forming on the cell wall[81].Additionally, CdS NPs were biosynthesized via Arabidopsis thaliana phytochelatin synthase-modified E. coli (CdS/AtPCS1-E. coli).…”

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confidence: 99%

Synthesis of Metal Nanoparticles by Microorganisms

Kato

Suzuki

2020

SSRN Journal

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Metal nanoparticles (NPs), with sizes ranging from 1-100 nm, are of great scientific interest because their functions and features differ greatly from those of bulk metal. Chemical or physical methods are used to synthesize commercial quantities of NPs, and green, energy-efficient approaches generating byproducts of low toxicity are desirable to minimize the environmental impact of the industrial methods. Some microorganisms synthesize metal NPs for detoxification and metabolic reasons at room temperature and pressure in aqueous solution. Metal NPs have been prepared via green methods by incubating microorganisms or cell-free extracts of microorganisms with dissolved metal ions for hours or days. Metal NPs are analyzed using various techniques, such as ultraviolet-visible spectroscopy, electron microscopy, X-ray diffraction, electron diffraction, energy-dispersive X-ray spectroscopy, and X-ray photoelectron spectroscopy. Numerous publications have focused on microorganisms that synthesize various metal NPs. For example

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“…For example, Au−Pd core−shell nanoparticles (NPs), which are outstanding candidates in the fields of materials science, chemistry, and biomedicine, have drawn the attention of researchers due to their unique biochemical activity and synergistic effects. 31 Au−Pd core−shell nanoparticles (NPs) biosynthesized using bacteria, 32 with excellent synergistic and tunable effects, have been employed in multiple biomedical applications, such as photothermal treatment, 33 biocatalysis, 34 biomimetic enzyme 35 and immunosensor production, 36 in vivo detection, 37 and for achieving antimicrobial effects. 38 However, existing studies on Au−Pd core−shell NPs still suffer from some deficiencies, such as (i) the absence of reports on the biological responses of biosynthesized NPs in the metabolomes of organisms; (ii) the very limited amount of Au−Pd NP-based metabolomics research; and (iii) the poor understanding of the intrinsic molecular mechanism and related metabolic pathways underlying Au−Pd−NP−organism interactions.…”

Section: Introductionmentioning

confidence: 99%

Exploring the Biological Effect of Biosynthesized Au–Pd Core–Shell Nanoparticles through an Untargeted Metabolomics Approach

Zhang

Xie

et al. 2021

ACS Appl. Mater. Interfaces

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The biosynthesis of Au–Pd core–shell nanoparticles (NPs) with wild-type Escherichia coli (Au–Pd/E. coli) is an excellent newly established, environmentally friendly synthetic method for the fabrication of nanomaterials compared to traditional chemosynthesis. However, there is insufficient detailed bioinformation on the compatibility, metabolic process, and mechanism of this approach. Metabolomics approaches have provided an excellent alternative to numerous bioinformatics approaches for shedding light on the biological response of an organism exposed to external stimuli at the molecular level. In this study, two different doses (8 and 80 μg/mL) of Au–Pd/E. coli were applied to treat human umbilical vein endothelial cells (HUVECs). Gas chromatography/mass spectrometry coupled with bioinformatics was used to analyze the changes in the HUVEC metabolome after treatment. The results indicated the occurrence of nonsignificant acute cytotoxicity based on cell proliferation and apoptosis analysis, while high concentrations (80 μg/mL) of Au–Pd/E. coli induced dramatic changes in energy metabolism, revealing a notable inhibition of the tricarboxylic acid (TCA) cycle along with the enhancement of glycolysis, the pentose phosphate pathway, fatty acid biosynthesis, and lipid accumulation, which was correlated with mitochondrial dysfunction. The metabolomics results obtained for this novel Au–Pd/E. coli–cell system could broaden our knowledge of the biological effect of Au–Pd/E. coli and possibly reveal material modifications and technological innovations.

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Improving biosynthesis of Au Pd core-shell nanoparticles through Escherichia coli with the assistance of phytochelatin for catalytic enhanced chemiluminescence and benzyl alcohol oxidation

Cited by 16 publications

References 57 publications

Enhanced Extracellular Synthesis of Gold Nanoparticles by Soluble Extracts from Escherichia coli Transformed with Rhizobium tropici Phytochelatin Synthase Gene

Enhanced Extracellular Synthesis of Gold Nanoparticles by Soluble Extracts from Escherichia coli Transformed with Rhizobium tropici Phytochelatin Synthase Gene

Synthesis of Metal Nanoparticles by Microorganisms

Exploring the Biological Effect of Biosynthesized Au–Pd Core–Shell Nanoparticles through an Untargeted Metabolomics Approach

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