Improving affinity of boronate capillary monolithic column for microextraction of glycoproteins with hydrophilic macromonomer

Zhou, Xiu-Jie; Mo, Chun-E; Chen, Meng; Huang, Yanping; Liu, Zhao‐Sheng

doi:10.1016/j.chroma.2018.11.005

Cited by 14 publications

(9 citation statements)

References 42 publications

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“…Interestingly, glycoproteins (and glycopeptides) can be recovered by lowering the pH, which simply reverses the cyclisation reaction. Several matrices have been functionalized with boronic acids, including monolithic columns (X. J. Zhou et al, 2018), magnetic beads (Yang et al, 2020) and gold particles (Tang et al, 2009), facilitating their integration with different experimental set‐ups.…”

Section: Enrichment Of Surfaceome Proteinsmentioning

confidence: 99%

Mass spectrometry and the cellular surfaceome

Pauwels

Fijałkowska

Eyckerman

et al. 2021

Mass Spectrometry Reviews

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The collection of exposed plasma membrane proteins, collectively termed the surfaceome, is involved in multiple vital cellular processes, such as the communication of cells with their surroundings and the regulation of transport across the lipid bilayer. The surfaceome also plays key roles in the immune system by recognizing and presenting antigens, with its possible malfunctioning linked to disease. Surface proteins have long been explored as potential cell markers, disease biomarkers, and therapeutic drug targets. Despite its importance, a detailed study of the surfaceome continues to pose major challenges for mass spectrometry‐driven proteomics due to the inherent biophysical characteristics of surface proteins. Their inefficient extraction from hydrophobic membranes to an aqueous medium and their lower abundance compared to intracellular proteins hamper the analysis of surface proteins, which are therefore usually underrepresented in proteomic datasets. To tackle such problems, several innovative analytical methodologies have been developed. This review aims at providing an extensive overview of the different methods for surfaceome analysis, with respective considerations for downstream mass spectrometry‐based proteomics.

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Section: Enrichment Of Surfaceome Proteinsmentioning

confidence: 99%

Mass spectrometry and the cellular surfaceome

Pauwels

Fijałkowska

Eyckerman

et al. 2021

Mass Spectrometry Reviews

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“…VPBA was attached to the monolith [102] and MCNT [92] via atom transfer radical polymerization. Acrylate APBA was co‐polymerized with other monomers and crosslinkers to synthesize boronate column in a single step [103]. 4‐Formyl phenyl boronic acid [104] and 5‐boronoisophthalic acid [105] were immobilized on amino dendritic layered monolith via Schiff base reaction [104] and NHS‐EDC chemistry [105] (Fig.…”

Section: Stationary Phase and Ligand Immobilizationmentioning

confidence: 99%

“…The preparation of hybrid monoliths involves the use of organic functional silanes along with alkoxysilanes. Both organic [92,[101][102][103][104][105][106] and hybrid monoliths [107] were used for glycopeptide or glycoprotein enrichment. Incorporation of ligands was performed either after the monolith was prepared or prior to in situ polymerization.…”

Section: Monolithmentioning

confidence: 99%

“…At pH 8.5, this affinity monolith had displayed high retention (affinity) for OVA over Lys (in a binary protein mixture). Zhou et al [103] had introduced oligo(ethylene glycol) methyl ether methacrylate (OEG) as the monomer to enhance the hydrophilicity of the boronate monolith column (250 μm id, 20 cm). The molar ratio of OEG: boronate (1 : 4) and the flow rate had played an important role in enhancing the glycoprotein adsorption and desorption as well.…”

Section: Chromatography Based Glycopeptide and Glcoprotein Enrichment...mentioning

confidence: 99%

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A review on recent advances in the enrichment of glycopeptides and glycoproteins by liquid chromatographic methods: 2016–Present

Kumari

Tetala

2021

Electrophoresis

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Among various protein post‐translational modifications (PTMs), glycosylation has received special attention due to its immense role in molecular interactions, cellular signal transduction, immune response, etc. Aberration in glycan moieties of a glycoprotein is associated with cancer, diabetes, and bacterial and viral infections. In biofluids (plasma, saliva, urine, milk, etc.), glycoproteins are low in abundance and are masked by the presence of high abundant proteins. Hence, prior to their identification using mass spectrometry methods, liquid chromatography (LC)‐based approaches were widely used. A general enrichment strategy involves a protein digestion step, followed by LC‐based enrichment and desorption of glycopeptides, and enzymatic excision of the glycans. The focus of this review article is to highlight the articles published since 2016 that dealt with different LC‐based approaches for glycopeptide and glycoprotein enrichment. The preparation of stationary phases, their surface activation, and ligand immobilization strategies have been discussed in detail. Finally, the major developments and future trends in the field have been summarized.

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“…The boronate affinity polymeric monoliths are generally in situ fabricated via free radical polymerization, epoxy–amino ring‐opening polymerization or ROMP using boronic acid ligands‐containing monomers and other crosslinkers as precursors. These monoliths exhibited various binding properties including binding pH, binding capacity, binding selectivity and binding affinity.…”

Section: Application Of Monolithic Materials For Enrichment and Fractmentioning

confidence: 99%

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

Wang

et al. 2019

Advanced Materials

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High‐performance liquid chromatography integrated with tandem mass spectrometry (HPLC–MS/MS) has become a powerful technique for proteomics research. Its performance heavily depends on the separation efficiency of HPLC, which in turn depends on the chromatographic material. As the “heart” of the HPLC system, the chromatographic material is required to achieve excellent column efficiency and fast analysis. Monolithic materials, fabricated as continuous supports with interconnected skeletal structure and flow‐through pores, are regarded as an alternative to particle‐packed columns. Such materials are featured with easy preparation, fast mass transfer, high porosity, low back pressure, and miniaturization, and are next‐generation separation materials for high‐throughput proteins and peptides analysis. Herein, the recent progress regarding the fabrication of various monolithic materials is reviewed. Special emphasis is placed on studies of the fabrication of monolithic capillary columns and their applications in separation of biomolecules by capillary liquid chromatography (cLC). The applications of monolithic materials in the digestion, enrichment, and separation of phosphopeptides and glycopeptides from biological samples are also considered. Finally, advances in comprehensive 2D HPLC separations using monolithic columns are also shown.

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Improving affinity of boronate capillary monolithic column for microextraction of glycoproteins with hydrophilic macromonomer

Cited by 14 publications

References 42 publications

Mass spectrometry and the cellular surfaceome

Mass spectrometry and the cellular surfaceome

A review on recent advances in the enrichment of glycopeptides and glycoproteins by liquid chromatographic methods: 2016–Present

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

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