Improvements in the use of the formaldehyde azo reaction for vitamin B1

Kinnersley, Henry Wulff; Peters, Rudolph

doi:10.1042/bj0321516

Cited by 20 publications

(3 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…at 450 for 4hr. to convert any thiamine pyrophosphate into thiamine (Kinnersley & Peters, 1938). The latter treatment is necessary since the pyrophosphate has about 30% more activity than an equimolar concentration of thiamine (Sarett & Cheldelin, 1944).…”

Section: Methodsmentioning

confidence: 99%

The de-repression of thiamine biosynthesis by adenosine a tool for investigating this biosynthetic pathway

Newell¹,

Tucker²

1966

Biochemical Journal

View full text Add to dashboard Cite

1. Growth of Salmonella typhimurium LT2 in the presence of adenosine was shown to cause enormous synthesis of thiamine in washed-cell suspensions. 2. Evidence that this was due to de-repression and not an accumulation of precursors was obtained by using a mutant blocked in the biosynthesis of the thiazole moiety, which showed a similarly large synthesis of the pyrimidine of thiamine. 3. The specific requirements for a source of energy, nitrogen and sulphur were investigated, and indicated new synthesis in this system.

show abstract

Section: Methodsmentioning

confidence: 99%

The de-repression of thiamine biosynthesis by adenosine a tool for investigating this biosynthetic pathway

Newell¹,

Tucker²

1966

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…Free aneurin was determined in skimmed milk by Jansen's (1936) method, while cocarboxylase was determined from the difference between the free aneurin and the aneurin present in the takadiastase (Parke, Davis and Co.) digest of the 2% triohloroacetio acid extract of skim milk. Takadiastase contains takaphosphatase (Kinnersley & Peters, 1938). Pepsin digestion of the trichloroacetic acid precipitate gave the protein-bound aneurin.…”

Section: Methods Of Analysismentioning

confidence: 99%

The partition of phosphorus and aneurin in relation to phosphatase in the colostrum and milk of the cow

Chanda

Owen

1952

J. Agric. Sci.

View full text Add to dashboard Cite

The partitions of phosphorus and aneurin were determined in the colostrum and in the transitional and mature milk of Ayrshire cows with the following results:1. The ratio of nitrogen to phosphorus was 11:1 in the first mammary secretion but dropped to a minimum value of 4·5:1 a week later. Thereafter the ratio increased throughout lactation attaining a value of 7·2:1 at the fortieth week. The first colostrum was very rich in both phosphorus and nitrogen.2. Phosphatase titre in colostrum was 154 units in the first post-partum secretion but decreased to 10 during the first five days of lactation. The titre fluctuated slightly during the next few days and increased gradually thereafter, attaining 189 units in the 38th week of lactation.3. Phosphatase was correlated positively with inorganic-P (r= +0·83) and negatively with both ester-P (r= −0·94) and lipid-P (r= −0·70), all P fractions being expressed as percentages of total P. The percentage of phosphorus present as casein was initially 19–21%, but was only 14% in late lactation.4. The first colostrum contained 74 μg. aneurin/100 ml. skim milk. This value became 35 in midlactation and only 28 in late lactation. The corresponding figures for cocarboxylase were 20, 5·7 and 2·3 μg./100 ml. respectively, and for protein-bound aneurin 14, 3·2 and 1·7 respectively. Both cocarboxylase and protein-bound aneurin were negatively correlated with phosphatase.

show abstract

“…Vitamin B, was estimated in skimmed milk before and after treatment with takadiastase (Houston, et al 1940). Takadiastase contains takaphosphatase (Kinnersley & Peters, 1938). Free vitamin B, was estimated by Jansen's method.…”

mentioning

confidence: 99%

The Composition of Human Milk with Special Reference to the Relation between Phosphorus partition and Phosphatase and to the Partition of Certain Vitamins

1951

View full text Add to dashboard Cite

Improvements in the use of the formaldehyde azo reaction for vitamin B1

Cited by 20 publications

References 4 publications

The de-repression of thiamine biosynthesis by adenosine a tool for investigating this biosynthetic pathway

The de-repression of thiamine biosynthesis by adenosine a tool for investigating this biosynthetic pathway

The partition of phosphorus and aneurin in relation to phosphatase in the colostrum and milk of the cow

The Composition of Human Milk with Special Reference to the Relation between Phosphorus partition and Phosphatase and to the Partition of Certain Vitamins

Contact Info

Product

Resources

About