Improvements in the Squash Technique for Plant Chromosomes

The Evolution of a Pair of Sibling Allotetraploid Species of Cobwebby Gilias (Polemoniaceae)

“…Iron was introduced from the dissecting needles during preparation. Slides were made permanent with Hoyer's medium by the method described by Beeks ( 1955) .…”

“…Slides were prepared by the propiono-carmine squash method and were made permanent by the use of Hoyer's medium (Beeks, 1955).…”

The Anatomy and Cytology of Pilostyles Thurberi Gray (Rafflesiaceae)

“…Vessel element size and percentage of stainable pollen protoplasts are based upon 100 observations. Cytological material was stained in propiono-carmine, and permanent squash amounts were made in Hoyer's solution as described by Beeks ( 1955). Chromosome numbers are based upon twenty observations.…”

Chromosome Counts of Compositae from the United States and Mexico

“…Florets were stained in iron-acetocarmine and were squashed in a drop of Hoyer's medium in modification of methods by Beeks (1955). Chromosome counts and behavior were ascertained from meiotic microsporocytes unless otherwise noted and were documented by camera lucida drawings.…”