Improvements in the extraction of cell electric properties from their electrorotation spectrum

A generic and label free method based on dielectrophoresis for the continuous separation of microorganism from whole blood samples

“…More recently, other methods have emerged, that can separate cells according to differences in their physical parameters: hydrodynamic sorting [12], lateral displacement filtration [13], magnetophoresis [14], electrophoresis [15], acoustophoresis [16] or optical forces [17], AC electrokinetic techniques [18]. In particular, the use of dielectrophoresis forces, electrorotation, and travelling wave dielectrophoresis showed to be quite efficient for cell trapping [19][20][21], cell separation [22][23][24]and cell characterization [25,26]. These label-free and non-contact techniques offer the advantage of being minimally invasive and less sensitive to the inherent pitfalls of surface-mediated capture.…”

Microfluidic Impedance Flow Cytometry Enabling High-Throughput Single-Cell Electrical Property Characterization

“…In electro-rotation, a rotating electric field is exerted to rotate a suspended single cell as a result of Maxwell-Wagner polarization. By measuring the rotating rate as a function of the applied frequency, this method is capable of collecting membrane permittivity and cytoplasm conductivity of single cells [ 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 ]. However, patch clamping and electrorotation rely on the precise manipulation and positioning of pipettes (patch clamping) or cells (electrorotation) which is time-consuming and labor-intensive [ 12 , 33 , 34 , 35 , 36 ].…”

Manipulation of gold coated microspheres using electrorotation

“…Electrorotation has been extensively applied to the micro-electromechanical systems(MEMS), especially to the manipulation of micro (or nano) particles and detemination of their surface properties, e.g., measuring electrorotation spectra of the polystyrene microspheres [6][7][8][9], membrane capacitance and conductance of cells [10][11][12][13], and cell viability for predicting completeness of the membrane [14,15]. The dielectric microparticles were extensively studied in tranditional electrorotation research, but the conductive microspheres were rarely studied in the AC electrokinetics.…”