Improvements in the Coating Technique of Radioautography

Kopriwa, Beatrix Markus; Leblond, C. P.

doi:10.1177/10.3.269

Cited by 929 publications

(216 citation statements)

References 0 publications

Supporting

Mentioning

206

Contrasting

Unclassified

Order By: Relevance

“…Tissue was serially sectioned (6 lm) and mounted on chromic-sulfuric acid cleaned slides (Chromerge, VWR Scientific, Mississauga, Ontario, Canada). Tissue sections used for light microscopic autoradiography (LM-autoradiography) were hydrated, oxidized in 0.5% aqueous periodic acid, rinsed in distilled water, air-dried, and dip-coated with Kodak NTB-2 nuclear track emulsion (Kodak, Rochester, NY, USA) (Kopriwa and Leblond, 1962). The emulsion was exposed at À20°C for 7-14 days, developed in Kodak D-19 Developer, rinsed in water, and fixed in Kodak Fixer.…”

Section: Light Microscopymentioning

confidence: 99%

KClO4 inhibits thyroidal activity in the larval lamprey endostyle in vitro

Manzon

Youson

2002

General and Comparative Endocrinology

View full text Add to dashboard Cite

An in vitro experimental system was devised to assess the direct effects of the goitrogen, potassium perchlorate (KClO 4 ), on radioiodide uptake and organification by the larval lamprey endostyle. Organification refers to the incorporation of iodide into lamprey thyroglobulin (Tg). Histological and biochemical evidence indicated that endostyles were viable at the termination of a 4 h in vitro incubation. A single iodoprotein, designated as lamprey Tg, was identified in the endostylar homogenates by polyacrylamide gel electrophoresis and Western blotting. Lamprey Tg was immunoreactive with rabbit anti-human Tg serum and had an electrophoretic mobility similar to that of reduced porcine Tg. When KClO 4 was added to the incubation medium, both iodide uptake and organification by the endostyle were significantly reduced relative to controls as determined by gamma counting, and gel-autoradiography and densitometry, respectively. Western blotting showed that KClO 4 significantly lowered the total amount of lamprey Tg in the endostyle. Based on the results of this in vitro investigation, we conclude that KClO 4 acts directly on the larval lamprey endostyle to inhibit thyroidal activity. These data support a previous supposition from in vivo experimentation that KClO 4 acts directly on the endostyle to suppress the synthesis of thyroxine and triiodothyronine, resulting in a decrease in the serum levels of these two hormones.

show abstract

Section: Light Microscopymentioning

confidence: 99%

KClO4 inhibits thyroidal activity in the larval lamprey endostyle in vitro

Manzon

Youson

2002

General and Comparative Endocrinology

View full text Add to dashboard Cite

show abstract

“…For electron microscopy, silver sections (-80 nm) were cut on a diamond knife with an LKB Ultrotome III (LKB Instruments, Inc ., Gaithersburg, MD) and collected on carbon-reinforced, nickel grids (I8). Slides were prepared for radioautography with Kodak NTB-2 according to the methods of Kopriwa and Leblond (19) . 2-wk exposure times were sufficient for all radioautography .…”

Section: Methodsmentioning

confidence: 99%

Radioautographic visualization and biochemical identification of O-phosphoserine- and O-phosphothreonine-containing phosphoproteins in mineralizing embryonic chick bone.

Landis¹,

Sanzone²,

Brickley-Parsons³

et al. 1984

The Journal of Cell Biology

View full text Add to dashboard Cite

We injected NaH 233P04 into normal 14-d-old embryonic chicks and examined the long bones by both radioautography and biochemical analyses from 10 to 240 min after the injection was completed . At 30 min, determination of the radiographic grain density revealed that 33p was concentrated principally in fibroblasts, preosteoblasts, and osteoblasts . With time, there was a progressive increase in the density of silver grains located over both the osteogenic cells and the regions of uncalcified (osteoid) and calcified extracellular organic matrices. Biochemical analyses identified 33p-O_phosphoserine as the major 33p component in glutaraldehyde-treated whole demineralized bone tissue and in EDTA-soluble, nondiffusible proteins extracted from the bones, both at the same time periods that 33P-induced silver grains were visualized by radioautography. 33p-O_phosphothreonine was also identified in experiments using a dosage of 10 mCi per embryo . The results provide the first combined direct biochemical and radioautographic identification that phosphoproteins are synthesized in bone and are located morphologically at the sites of mineralization . The data provide further evidence that phosphoproteins play a critical role in the biological calcification of vertebrate tissues.

show abstract

“…The specificity of the antisera was confirmed by absorption control experiments with homologous and heterologous antigen prior to staining. Subsequent to immunohistochemical staining, these slides were radioautographed using Kodak NTB3 liquid emulsion (Eastman Kodak Company, Rochester, NY) according to Kappriwa and Leblond (1962). After exposure a t 4°C for 28 days, the slides were developed and counterstained with hematoxylin.…”

Section: Immunohistochemistry and Radioautographymentioning

confidence: 99%

Growth dynamics of pancreatic islet cell populations during fetal and neonatal development of the rat

Kaung

1994

Developmental Dynamics

136

View full text Add to dashboard Cite

Rats from 18 days fetal to 28 days neonatal ages were studied for the total population sizes and cell proliferation activities of insulin secreting B cells, glucagon secreting A cells, somatostatin secreting D cells, and pancreatic polypeptide secreting PP cells. Cell population sizes were assessed by morphometric quantitation of immunohistochemically stained cells by a linear scanning method and cell proliferation activities were estimated by r3HI-thymidine labeling indices of these cell populations. There was a continuous increase in population sizes for all 4 islet cell types, with the fastest increase occurring in the last 4 days of gestation. The accelerated growth of these islet cell populations during late gestation was accomplished by a high cell proliferative activity at 20-22 days of gestation and a large influx of undifferentiated epithelial cells differentiating into the specific islet cell populations during this period. There was a reduction of population growth and cell proliferation for all islet cell types during the first 3-4 days of life. Growth activities continued for all islet cell populations after the 4th postnatal day, with a renewed acceleration in growth activities for B and A cells at this time. After the 10th neonatal day, the cell proliferation and total population growth continued at slow rates for all 4 islet cell types. The contribution from undifferentiated epithelial cells into the specific islet cell populations was negligible for B and A cells but continued at a low rate for PP and D cells during the first 10 days after birth. For B and A cell populations, there was a possibility that some cell loss occurred during the first 10 days of neonatal life. These dynamic changes of the growth characteristics provide a basis for understanding the abnormal growth of the endocrine pancreas. 0 1994 Wiley-Liss, Inc.

show abstract

Improvements in the Coating Technique of Radioautography

Abstract: Time coating techumique of radlioatntogrcuphy' devised with B#{233}langer iii 1946 (2) oonsists iii covering radioactive sections with fluid enmuulsion.

Cited by 929 publications

References 0 publications

KClO4 inhibits thyroidal activity in the larval lamprey endostyle in vitro

KClO4 inhibits thyroidal activity in the larval lamprey endostyle in vitro

Radioautographic visualization and biochemical identification of O-phosphoserine- and O-phosphothreonine-containing phosphoproteins in mineralizing embryonic chick bone.

Growth dynamics of pancreatic islet cell populations during fetal and neonatal development of the rat

Contact Info

Product

Resources

About