Improvement Strategies in Ovine Artificial Insemination

Anel, L.; Álvarez, M.; Martínez‐Pastor, Felipe; García-Macías, V.; Anel, E.; Paz, P. de

doi:10.1111/j.1439-0531.2006.00767.x

Cited by 82 publications

(66 citation statements)

References 114 publications

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“…The shorter IML was observed for parities of 1 and 3 which are not significantly different with 163.4 and 164 days, respectively. This confirmed the decrease of fertility with parity for many species (Anel et al, 2006;Grimmard et al, 2006). As to litter size, the longest IML corresponded to a litter size of 1 with 164.9 days.…”

Section: Datasupporting

confidence: 55%

Non-genetic sources of variation of milk production and reproduction and interactions between both classes of traits in Sicilo-Sarde dairy sheep

Meraï

Gengler

Hammami

et al. 2014

Animal

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This work aimed to study the sources of variation in productive and reproductive traits of the dairy Sicilo-Sarde ewes and to further investigate the interaction between both classes of traits. After edits, a database containing 5935 lactation records collected during 6 successive years in eight dairy flocks in the North of Tunisia was used. Total milked milk (TMM) in the milking-only period was retained as productive trait. The interval from the start of the mating period to the subsequent lambing (IML) and the lambing status (LS) were designed as reproductive traits. Sicilo-Sarde ewes had an average TMM of 60.93 l (±44.12) during 132.8 days (±46.6) after a suckling period of 100.4 days (±24.9). Average IML was 165.7 days. In a first step, the major factors influencing milk production and reproductive traits were determined. The significant sources of variation identified for TMM were: flock, month of lambing, year of lambing, parity, suckling length, litter size and milking-only length. Flock × month of the start of the mating period, parity, year of mating and litter size were identified as significant factors of variation for IML, while flock × month of the start of the mating period, parity and year of mating were identified as significant sources of variation for LS. In a second step, variance components were estimated using a three traits threshold mixed model, which combined LS as categorical trait and TMM and IML as continuous traits. Repeatability estimates were 0.21 (±0.03) for TMM, 0.09 (±0.02) for IML, and 0.10 (±0.05) for LS. Moreover, TMM and IML were found to be favorably associated for the flock × year of lambing effect (−0.45 ± 0.18) but unfavorably associated for the animal effect (0.20 ± 0.09).

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Section: Datasupporting

confidence: 55%

Non-genetic sources of variation of milk production and reproduction and interactions between both classes of traits in Sicilo-Sarde dairy sheep

Meraï

Gengler

Hammami

et al. 2014

Animal

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“…Among the evaluation parameters for sperm characteristics, motility, especially progressive motility (PM), is commonly believed to be one of the most important characteristics associated with the fertilizing ability of sperm [18]. However, the values of motility and PM are usually determined in subjective manners.…”

Section: Discussionmentioning

confidence: 99%

Effects of Semen Extenders and Storage Temperatures on Characteristics of Frozen-Thawed Bryde's (Balaenoptera edeni) Whale Spermatozoa

Hiwasa

Suzuki

Watanabe

et al. 2009

Journal of Reproduction and Development

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Abstract. The present study investigated effects of three semen extenders and storage temperatures on post-thaw characteristics of Bryde's whale spermatozoa. Spermatozoa were collected from the vasa deferens of three mature Bryde's whales captured during the Japanese whale research in the north-west Pacific (May to August 2007) after death. The three semen extenders used for freezing were 1) a commercialized synthetic extender (AndroMed: AM), 2) Trisbased + 10% bovine serum albumin (BSA) and 3) Tris-based + egg yolk (EY). The sperm samples from the three whales were frozen with the three extenders, and the post-thaw spermatozoa were stored at three different temperatures (35 C; 20-25 C, room temperature; and 5 C) for 0, 6, 12, 24, 48 and 96 h. At each time-point, total and progressive motility (PM), viability (live or dead), the hypo-osmotic test, defective acrosomes and malformation were examined. Immediately after thawing, AM resulted in similar recovery rates (60.4 and 83.3%) in 2 of the 3 whales examined and had comparable post-thaw recovery rates to those obtained using the EY and BSA extenders. Immediately after thawing, the proportion of PM in EY (17.6%) was higher (P<0.05) than that in BSA (15.0%). In the hypo-osmotic test, the proportions of AM (26.0%) and BSA (25.2%) were higher (P<0.05) than that of EY (17.3 %). The three extenders had similar viabilities (36.7, 37.9 and 32.1%, respectively), but the viability of BSA was higher (P<0.05) than that of EY. The present study showed that a synthetic semen extender, AndroMed, could be used for cryopreservation of whale spermatozoa in addition to Tris-based extenders containing bovine serum albumin or egg yolk. Storage of the post-thaw Bryde's whale spermatozoa was better at 5 C than at room temperature or 35 C. The frozen-thawed Bryde's whale spermatozoa maintained their motility and viability for at least two days at room temperature and for four days at 5 C. [5] used Bryde's (Balaenoptera edeni) whale spermatozoa freshly-diluted with m-PBS and stored at 4 C for in vitro fertillization (IVF) in the field. However, it has not been demonstrated yet how long the viability of frozen-thawed spermatozoa is maintained and what the most appropriate temperature is for post-thaw storage in baleen whales, including Bryde's whales. For domestic animals including sheep, post-thaw semen is usually maintained at 37 C for examinations of their motility and viability, but the body temperatures (35.5 to 37 C) of dolphins and whales are lower than those of other mammals, and no one has measured the body temperatures of live baleen whales, including Antarctic minke and Bryde's whales.There has been recent movement to eliminate all animal ingredients, such as egg yolk, milk and even bovine serum albumin (BSA), from semen extenders. Our recent studies [6-8] on artificial insemination (AI) in sheep have clearly shown that a soybean-based semen extender (AndroMed) is useful and comparable to the conventional semen extenders containing egg yolk or BSA. Fukui et al. [6] compared ...

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“…Since then, technologies related to laparoscopic AI have spread throughout the world, and at present, laparoscopic AI is the only method that ensures intrauterine application of frozenthawed semen with satisfactory fertility in sheep under field conditions [11][12][13][14][15]. However, laparoscopic AI has problems related to its complexity, high equipment costs and the need for trained technicians and other problems related to animal welfare [20]. The best insemination method is a simple, practical method, such as vaginal insemination (shot in dark).…”

Section: Discussionmentioning

confidence: 99%

Fertility after Artificial Insemination Using a Soybean-Based Semen Extender in Sheep

Fukui

Kohno

Togari

et al. 2008

Journal of Reproduction and Development

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Abstract. The present study aimed to compare the fertility of ewes intrauterinally inseminated with frozen-thawed semen using a soybean-based semen extender (AndroMed) with those of ewes intrauterinally inseminated with frozenthawed semen using a Tris-based extender containing either egg yolk or BSA. Suffolk ewes (n=104) were treated with an intravaginal sponge containing 40 mg fluoroprogesterone acetate (FGA) for 12 days and an intramuscular injection of 500 IU equine chorionic gonadotropin to induce estrus and ovulation during the non-breeding season (July, 2007). Intrauterine insemination was carried out 40-46 h after removal of the FGA sponge (n=90), regardless of the incidence of estrus. The pregnancy rates were not significantly different among the semen extenders containing egg yolk (64.5%) or BSA (58.6%) and AndroMed extender (56.7%). The lambing rates (64.5, 55.2 and 56.7% for the semen extenders containing egg yolk, BSA and AndroMed, respectively) and prolificacy (1.59 to 1.75) were also not significantly different. The present results indicate that an egg yolk-containing semen extender can be replaced with the non-animal derived extender AndroMed, which could be used for intrauterine insemination using frozen-thawed ram semen without reducing fertility. Key words: Artificial insemination, Fertility, Semen extender, Sheep (J. Reprod. Dev. 54: [286][287][288][289] 2008) gg yolk-based semen extenders have been widely utilized for cryopreservation of semen from farm animals including sheep [1][2][3]. However, it is not always easy to prepare semen extenders consistent with quality standards because of the individual quality differences inherent in egg yolk due to the numbers of days after laying and the storage period. Also, addition of egg yolk reduces the acrosome integrity of goat spermatozoa [4], and high egg yolk concentrations reduce the post-thawing viability of ejaculated spermatozoa in several species, such as goats [5], rams [1] and water buffaloes [6]. Furthermore, there has been movement recently to eliminate all animal ingredients, including egg yolk, milk or bovine serum albumin (BSA), in order to design a defined semen extender. Removal of chicken egg yolk from semen extenders would provide several advantages, such as improved consistency in the components of semen extenders and elimination of hygienic risks. Therefore, development of a synthetic semen extender free of animal sources has been desired. A soybean lecithin-based extender (AndroMed; Minitub, Tiefenbach, Germany) has been developed and utilized for bovine [7][8][9] and mountain gazelle semen [10]. Fresh and frozen ram semen diluted with a synthetic semen extender, AndroMed, has been inseminated with satisfactory fertility results in Norway (Paulenz H.: personal communication).Low fertility (20-30%) in ewes inseminated with frozen semen into the cervical orifice, an ordinal deposition site for artificial insemination (AI) in sheep, has not been applied fully on the field, except for intrauterine AI using laparoscopy (60-80% in...

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Improvement Strategies in Ovine Artificial Insemination

Cited by 82 publications

References 114 publications

Non-genetic sources of variation of milk production and reproduction and interactions between both classes of traits in Sicilo-Sarde dairy sheep

Non-genetic sources of variation of milk production and reproduction and interactions between both classes of traits in Sicilo-Sarde dairy sheep

Effects of Semen Extenders and Storage Temperatures on Characteristics of Frozen-Thawed Bryde's (Balaenoptera edeni) Whale Spermatozoa

Fertility after Artificial Insemination Using a Soybean-Based Semen Extender in Sheep

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