Improvement of wound healing by regulated oxygen-enriched negative pressure-assisted wound therapy in a rabbit model

Abstract

SummaryBackground. Development of drug therapies and other techniques for wound care have resulted in significant improvement of the cure rate and shortening of the healing time for wounds. A modified technique of regulated oxygen-enriched negative pressure-assisted wound therapy (RO-NPT) has been reported. Aim. To evaluate the efficacy and impact of RO-NPT on wound recovery and inflammation. Methods. Infected wounds were established on 40 adult female white rabbits, which were then randomized to one of four groups: O 2 group, regulated negative pressureassisted wound therapy (RNPT) group, regulated oxygen-enriched negative pressureassisted wound therapy (RO-NPT) group and healthy control (HC) group. Each day, the O 2 group was treated with a constant oxygen supply (1 L/min) to the wound, while the RNPT group was treated with continuous regulated negative pressure (70 AE 5 mmHg) and the RNPT + O 2 group was treated with both. The HC group was treated with gauze dressing alone, which was changed every day. Leucocyte count, colony count and wound-healing rate were calculated. Levels of tumour necrosis factor (TNF)-a, interleukin (IL)-1b and IL-8 were evaluated by ELISA. Results. RO-RNPT significantly decreased bacterial count and TNF-a level, and increased the wound-healing rate. IL-1b, IL-8 and leucocyte count had a tendency to increase in the early phase of inflammation and a tendency to decrease in the later phase of inflammation in the RO-RNPT group. Conclusions. RO-NPT therapy assisted wound recovery and inflammation control compared with the RNPT and oxygen-enriched therapies. RO-NPT therapy also increased levels of IL-1b and IL-8 and attenuated expression of TNF-a in the early phase of inflammation.

Investigating Inflammatory Markers in Wound Healing: Understanding Implications and Identifying Artifacts

“…For instance, claiming that a treatment can effectively heal a wound by reducing the release of pro-inflammatory factors at specific times raises serious questions because effective wound healing requires the early release of pro-inflammatory markers. Furthermore, the crucial role of the pro-inflammatory cytokines (such as IL-8 and IL-6) in the late stages of wound healing, such as re-epithelization and remodeling, cannot be underestimated; thus, significantly modulating the release of the pro-inflammatory markers is not always in favor of enhanced wound healing, and the possible impact of these materials on releasing anti-inflammatory factors should be considered. − Likewise, the modulatory effects of materials on the release of anti-inflammatory markers do not sufficiently illustrate their potential for wound healing; in order to substantiate the therapeutic efficacy, such evaluations should be supplemented by examining their impact on a wide range of other pro- and anti-inflammatory markers.…”

Minimizing treatment complexity of combat-related soft tissue injuries using a dedicated tension relief system and negative pressure therapy augmented by high-dose in situ antibiotic therapy and oxygen delivery: a retrospective study

“…Low oxygen partial pressures develop in wounds treated using conventional negative pressure wound therapy (NPWT) systems, restricting their suitability for wounds with significant anaerobic contamination [ 8 ]. Supplemental oxygen reverses the reduced oxygen partial pressure in the wound and may inhibit anaerobic bacterial growth [ 9 , 10 ]. Concurrent hydrodynamic irrigation accelerates the evacuation of infectious material from the wound [ 11 ].…”

Case Report: Delayed Primary Wound Closure After Extensive Abdominal Wall Resection for Infection and Malignancy Using TopClosure®

“…This feature is augmented by the use of a semi-transparent (off-white) sponge that permits easy visualization of bleeding (and infection). Supplemental oxygen reverses the reduced partial pressure of oxygen in the wound induced by conventional vacuum systems, and may inhibit anaerobic bacterial growth ( 4 , 5 ). Concurrent antibiotic irrigation of the wound hydrodynamically accelerates the evacuation of infectious material from the wound ( 6 ) and may effectively decontaminate the wound ( 7 ).…”