Improvement of wheat drought and salt tolerance by expression of a stress-inducible transcription factorGmDREB of soybean (Glycine max)

Gao, Shiqing; Xu, Huijun; Cheng, Xiangdong; Chen, Ming; Xu, Zhao‐Shi; Li, Lian-Cheng; Ye, Xingguo; Du, Liangcheng; Hao, Xiaoyan; Ma, Yupo

doi:10.1007/bf02899641

Cited by 25 publications

(17 citation statements)

References 32 publications

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“…This indicates that overexpression of the transcript factor GmDREB1 affects the expression of miRNAs in wheat seeds. Most of the target genes of the differentially expressed miRNAs between GM wheat and the non-GM acceptor were associated with abiotic stress, in accordance with the product concept of the GM wheat line T349 [17, 18]. These results provided useful data for a bio-safety assessment of GM crops and valuable information for wheat miRNA research.…”

Section: Introductionsupporting

confidence: 70%

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GmDREB1 overexpression affects the expression of microRNAs in GM wheat seeds

et al. 2017

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MicroRNAs (miRNAs) are small regulators of gene expression that act on many different molecular and biochemical processes in eukaryotes. To date, miRNAs have not been considered in the current evaluation system for GM crops. In this study, small RNAs from the dry seeds of a GM wheat line overexpressing GmDREB1 and non-GM wheat cultivars were investigated using deep sequencing technology and bioinformatic approaches. As a result, 23 differentially expressed miRNAs in dry seeds were identified and confirmed between GM wheat and a non-GM acceptor. Notably, more differentially expressed tae-miRNAs between non-GM wheat varieties were found, indicating that the degree of variance between non-GM cultivars was considerably higher than that induced by the transgenic event. Most of the target genes of these differentially expressed miRNAs between GM wheat and a non-GM acceptor were associated with abiotic stress, in accordance with the product concept of GM wheat in improving drought and salt tolerance. Our data provided useful information and insights into the evaluation of miRNA expression in edible GM crops.

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Section: Introductionsupporting

confidence: 70%

“…The transgenic wheat line T349, transformed with GmDREB1 from soybean, showed strong tolerance to drought and salt stresses [17, 18]. The transgenic and non-transgenic wheat varieties were planted in a field with water-saving measures in the Jinan province of China.…”

Section: Methodsmentioning

confidence: 99%

GmDREB1 overexpression affects the expression of microRNAs in GM wheat seeds

et al. 2017

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“…In soybean, many studies have reported that overexpression of TF genes could alter salt tolerance. Several studies reported that the GmDREB genes are induced by various types of stresses, including salt stress (Gao et al, 2005;Chen et al, 2007;Mizoi et al, 2013;Yamasaki & Randall, 2016). GmDREB2 can specifically bind to the DRE motif, and ectopically expressing the GmDREB2 gene in Arabidopsis enhances its tolerance to high salinity (Chen et al, 2007).…”

Section: Transcriptional Control Of Salt Tolerance In Soybeanmentioning

confidence: 99%

“…GmDREB2 can specifically bind to the DRE motif, and ectopically expressing the GmDREB2 gene in Arabidopsis enhances its tolerance to high salinity (Chen et al, 2007). Similarly, wheat transgenic lines harboring either Ubi::GmDREB1 or rd29A:: GmDREB1 construct showed enhanced salt tolerance (Gao et al, 2005). More recently, several GmERF genes were identified and characterized with regard to their functions in soybean salt tolerance (Zhang et al, 2009;Zhang et al, 2010;Zhai et al, 2013aZhai et al, , 2013b.…”

Section: Transcriptional Control Of Salt Tolerance In Soybeanmentioning

confidence: 99%

Adaptive Mechanisms of Soybean Grown on Salt‐Affected Soils

Cao

Liu

et al. 2017

Land Degrad Dev

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Soybean (Glycine max) is an economically important pulse crop for livestock feed, human consumption and industrial use as a source for production of biodiesel. However, soybean yield is detrimentally affected by soil salinity around the world. To cope with soil salinization, in addition to soil remediation, approaches used in genetically modifying soybean genotypes to increase their productivity under saline conditions are also of importance. Thus, it is crucial to unravel the mechanisms controlling soybean responses to soil salinity in order to improve soybean performance with high salinity tolerance. Knowledge of the regulatory network for salt tolerance in model plants, including Arabidopsis, and the publically available soybean genome sequence has accelerated identification and functional analyses of genes regulating soybean responses to high salinity. This review presents an update of recent works on genetic studies of salt tolerance and the mechanisms regulating soybean responses and tolerance to soil salinity. We also discuss the effort and progress that have been made in the last decade toward developing high salt‐tolerant soybean varieties with improved productivity. Copyright © 2017 John Wiley & Sons, Ltd.

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“…Soybean GmDREB2 functioned as transcriptional activator and was useful in improving plant tolerance to abiotic stress (Chen et al, 2007). Arabidopsis AtDREB1A conferred drought and salt tolerance in peanut, which may be attributed to enhancement of proline content and superoxide dismutase (SOD) activity in soybean and chrysanthemum (Hong et al, 2006;Shiqing et al, 2005). In addition, ERF proteins are reported to regulate the biosynthesis of metabolites, such as Jasmonate, gibberellin, ethylene, lipid and wax, to enhance the tolerance to environmental stresses (Zhang et al, 2012).…”

Section: Ethylene Response Factor (Erf) Tfmentioning

confidence: 99%

Genetic Engineering of Crop Plants for Drought Tolerance:Role of Transcription Factors

David

Ceasar

Thirugnanasambantham³

et al. 2016

sijbs

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Agricultural production is severely affected by various abiotic stresses including drought. Development of crop plants tolerant to drought is an urgent need to increase crop productivity to feed the growing population. It is imperative to understand the adaptive mechanism of plants to drought especially the type and expression levels of drought responsive genes. Several genes upregulated during abiotic stress have been exploited to develop drought tolerant plants. Genetic engineering of crop plants with stress responsive genes has been an effective method of generating drought tolerant plants. But expressions of these stress responsive genes are regulated by several transcription factors (TFs). TFs are DNA binding proteins that bind to the specific sequence motif and modulate the levels of gene expression. They may function as an activator, or repressor for the expression of specific genes. Transgenic plants expressing drought responsive TFs have been shown to improve drought tolerance in many crop plants. In this review, we have discussed the details on TFs used for developing various transgenic plants and also analyzed the potential of TFs for further transgenic studies. The future direction to exploit TFs for sustainable crop production under drought is also highlighted.

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Improvement of wheat drought and salt tolerance by expression of a stress-inducible transcription factorGmDREB of soybean (Glycine max)

Cited by 25 publications

References 32 publications

GmDREB1 overexpression affects the expression of microRNAs in GM wheat seeds

GmDREB1 overexpression affects the expression of microRNAs in GM wheat seeds

Adaptive Mechanisms of Soybean Grown on Salt‐Affected Soils

Genetic Engineering of Crop Plants for Drought Tolerance:Role of Transcription Factors

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