Improvement of welan gum biosynthesis and transcriptional analysis of the genes responding to enhanced oxygen transfer by oxygen vectors in Sphingomonas sp.

Zhu, Ping; Dong, Shuhao; Li, Sha; Xu, Xiaoying; Xu, Hong

doi:10.1016/j.bej.2014.06.011

Cited by 33 publications

(14 citation statements)

References 36 publications

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“…Zhu reported that the activity of the key enzymes in TCA cycle in Sphingomonas sp . exhibited positive variations under enhanced DOC and oxygen transfer rates induced by oxygen‐vector ( n ‐dodecane and n ‐hexadecane) . Compared with the control in our study, significantly higher activities of TCA enzymes was observed in enhanced DOC, which were induced by oxygen‐vectors.…”

Section: Resultsmentioning

confidence: 43%

“…These results indicated that though part of the added oleic acid was consumed as co‐substrate for CA synthesis by Y. lipolytica SWJ‐1b, the low consumption rate insured oleic acid the function of transfering oxygen to the medium during CA production. Studies have confirmed that the addition of oxygen‐vector in culture broth enabled the increase of volumetric oxygen transfer coefficient ( k L a), which enhanced oxygen diffusion rate from the air to the broth to increase DOC in the culture broth , and hence improved the production of CA.…”

Section: Resultsmentioning

confidence: 92%

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Improved production of citric acid by Yarrowia lipolytica using oleic acid as the oxygen‐vector and co‐substrate

Liu

Xia

et al. 2016

Engineering in Life Sciences

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Improved production of citric acid by Yarrowia lipolytica using oleic acid as the oxygen-vector and co-substrate Yarrowia lipolytica is able to secrete large amounts of citric acid (CA), which is greatly affected by the dissolved oxygen concentration (DOC) in the fermentation medium. In this study, oleic acid was selected as oxygen-vector to improve DOC during CA fermentation. When 2% (v/v) of oleic acid was added to the culture broth, higher DOC (>42.1%) was determined throughout the CA synthesis phase. The yield of CA reached a maximum of 32.1 g/L (25.4% higher than the control) and the biomass was 8.8 g/L. The substrate uptake rate, products formation rate and key enzyme activities were also determined, and the results indicated that CA synthesis was strengthened with oleic acid addition. Furthermore, it was detected that oleic acid could be assimilated by the cells, which means that oleic acid could be served both as oxygen-vector and co-substrate for CA synthesis by Y. lipolytica. In a bioreactor with working volume of 3 L, the highest concentration of CA reached to 36. 4 g/L in the presence of 2% (v/v) oleic acid after 192 h of fermentation. These results confirmed that oleic acid could be applied in the large-scale production of CA by Y. lipolytica.

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Section: Resultsmentioning

confidence: 43%

Section: Resultsmentioning

confidence: 92%

Improved production of citric acid by Yarrowia lipolytica using oleic acid as the oxygen‐vector and co‐substrate

Liu

Xia

et al. 2016

Engineering in Life Sciences

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“…Thus, we believed that, was in the case of the three cultures on biomass concentration and lipid content were different not due to nitrogen exhaustion but presumably to the oxygen availability. So, oxygen has a great importance in the R. toruloides CECT 1499 yeast cultures and high oxygen promotes lipogenesis and consequently lipid accumulation, as a result of the up-regulation of enzymes involved in lipid biosynthesis such as malic enzyme and ATP-citrate lyase [50][51][52]. When scaled-up from 250 mL Erlenmeyer (low oxygen availability) to 2L-STR (control) the lipid content increased from 22 to 47% w/w and the K L a increased 6.8 times.…”

Section: Effect Of Oxygen Availability In Lipid Accumulation (High Aementioning

confidence: 99%

“…That genes encoded enzymes like glucokinase, citrate synthase, malate dehydrogenase, and glucose-6phosphate dehydrogenase [51,52]. In presence of n-dodecane lipid productivity was stimulated and reached 0.20 and 0.21 g/L day, with 1 and 2% of n-dodecane, respectively, while in culture without addition of n-dodecane, lipid productivity dropped to 0.16 g/L day.…”

Section: Effect Of N-dodecane In Lipid Accumulation (N-dodecane Appromentioning

confidence: 99%

“…In an aerobic culture, particularly for R. toruloides CECT 1499 yeast cultures, several biochemical reactions including carbon consumption, lipid production, and cell proliferation required large amounts of oxygen. So, oxygen has a great importance in the R. toruloides CECT 1499 yeast cultures and high oxygen promotes lipogenesis and consequently lipid accumulation, as a result of the up-regulation of enzymes involved in lipid biosynthesis such as malic enzyme and ATP-citrate lyase [50][51][52].…”

Section: Effect Of Oxygen Availability In Lipid Accumulation (High Aementioning

confidence: 99%

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A new combined approach to improved lipid production using a strictly aerobic and oleaginous yeast

Guerreiro

Constantino

Lima-Costa

et al. 2018

Engineering in Life Sciences

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Microbial lipids have potential applications in energy, and food industry, because most of those lipids are triacylglycerol with long‐chain fatty‐acids that are comparable to conventional vegetable oils and can be obtained without arable land requirement. Rhodosporidium toruloides is a strictly aerobic strain, where oxygen plays a crucial role in growth, maintenance, and metabolite production, such as lipids and carotenoids. Dissolved oxygen concentration is one of the major factors affecting yeast physiological and biochemical characteristics. In this context, different approaches have been developed to increase available oxygen by the increasing the aeration and the addition of an oxygen‐vector. The growth of R. toruloides in 2‐L mechanical stirred tank reactor equipped with 1 or 2 porous spargers and a 70 C/N ratio, revealed a lipid content of 0.47 and 0.52 g/g and a lipidic productivity of 0.16 and 0.17 g/L day, respectively. The oxygen‐vector addition, increased the lipidic productivity for 0.20 g/L day and a lipid contend of 0.51 g of lipids/g of biomass. The combined approach, combining high aeration (AA), and 1% of n‐dodecane addition (DA), produced a significant improvement in the lipid accumulation (62%, w/w), when compared with the DA (51%, w/w) and the AA (52%, w/w) approaches. The increasing of lipids accumulation and smaller culture time are key factors for the success of scale‐up and profitability of a bioprocess.

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Fed‐batch fermentation strategy for efficient welan gum production by Sphingomonas sp. FM01

Liu,

He,

Wei

et al. 2024

J Sci Food Agric

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BACKGROUNDAs a novel type of extracellular polysaccharide produced by Sphingomonas sp., welan gum has been widely applied in various fields because of its excellent properties. The study has improved the fermentation process.ResultsThe initial sucrose concentration, temperature and stirring speed were set to 20 g L−1, 33 °C and 400 rpm, respectively, and 13.3 g L−1 sucrose was added at 24, 40 and 56 h. The temperature and stirring speed were then set at 28 °C and 600 rpm from 24 to 48 h and 28 °C and 600 rpm from 48 to 72 h, respectively. As a result, welan gum production, dry cell weight, sucrose conversion rate and viscosity were correspondingly increased to 38.60 g L−1, 5.47 g L−1, 0.64 g g−1 and 3779 mPa·s, respectively. In addition, the mechanism by which fermentation strategy promotes welan gum synthesis was investigated by transcriptome analysis.CONCLUSIONImproving respiration and ATP supply, reducing unnecessary protein synthesis, and alleviating competition between cell growth and welan gum synthesis contribute to promoting the fermentation performance of Sphingomonas sp., thus providing a practical strategy for efficient welan gum production. © 2024 Society of Chemical Industry.

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Improvement of welan gum biosynthesis and transcriptional analysis of the genes responding to enhanced oxygen transfer by oxygen vectors in Sphingomonas sp.

Cited by 33 publications

References 36 publications

Improved production of citric acid by Yarrowia lipolytica using oleic acid as the oxygen‐vector and co‐substrate

Improved production of citric acid by Yarrowia lipolytica using oleic acid as the oxygen‐vector and co‐substrate

A new combined approach to improved lipid production using a strictly aerobic and oleaginous yeast

Fed‐batch fermentation strategy for efficient welan gum production by Sphingomonas sp. FM01

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