Improvement of Virus Safety of a S/D-Treated Factor VIII Concentrate by Additional Dry Heat Treatment at 100°C

Dichtelmüller, H.; Rudnick, Dorothea; Breuer, Brenda; Kotitschke, R.; Kloft, M.; Darling, Allan J.; Watson, E. A.; Flehmig, Bertram; Lawson, Sarah K.; Frösner, Gert

doi:10.1006/biol.1996.0016

Cited by 23 publications

(16 citation statements)

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“…As previously found, the presence of a minimum of 0.7% residual moisture in the concentrate is the ultimate requirement for achieving significant virus reduction (25,41).…”

Section: Discussionmentioning

confidence: 63%

Development and Applications of Surface Plasmon Resonance-Based von Willebrand Factor– Collagen Binding Assay

Saenko

Kannicht

Löster

et al. 2002

Analytical Biochemistry

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“…As previously found, the presence of a minimum of 0.7% residual moisture in the concentrate is the ultimate requirement for achieving significant virus reduction (25,41).…”

Section: Discussionmentioning

confidence: 63%

Development and Applications of Surface Plasmon Resonance-Based von Willebrand Factor– Collagen Binding Assay

Saenko

Kannicht

Löster

et al. 2002

Analytical Biochemistry

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“…HAV is also stabilized by these additives, which means that pasteurization sometimes only results in a reduction in the infectivity of HAV in the order of 3-5 log10 [47][48][49][50][51][52]. Heat treatment of a lyophilized product with approximately 7% residual moisture is another effective method of inactivating HAV (10 hours at 60°C or a combination of 10 h at 60 °C and 2 h at 80°C ) [53], as is dry heat treatment (residual moisture approximately 1% ) for 30 min at 100 °C [54] or 72 h at 80 °C [55]. Because HAV does not have a lipid-containing viral envelope, the S/D method [56], which is very effective for inactivating enveloped viruses such as HIV, HBV and HCV, is completely ineffective.…”

Section: Methods For Removal and Inactivation Of The Infectious Agentmentioning

confidence: 99%

Hepatitis A Virus

2001

Transfus Med Hemother

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1 Current Knowledge about the Pathogen 1.1 Characteristics of Hepatitis A Virus The clinical features of hepatitis A have been known for a long time. However, the pathogen (hepatitis A virus (HAV)) was firstly identified in 1973 with the aid of immune electron microscopy [1]. HAV is classified in the family of Picornaviridae , genus Hepatovirus . It is a small, spherical, non-enveloped virus with a diameter of approximately 30 nm. The capsid of the viral particle is made up of 60 copies of the three viral structural proteins VP1–VP3. The genome consists of a single- stranded (+)sense RNA molecule of 7,500 bases in length. The 5'' end of the genome is covalently bound to a small protein (VPg). The genome serves as messenger RNA and can be divided into three regions: a long open-reading frame (ORF) is flanked by the non-coding 5'' region and the non-coding 3'' region. The ORF encodes a polyprotein which is cleaved by proteolysis into the mature viral proteins. Two thirds of the genome code for non-structural proteins with enzymatic and regulatory functions. Translation is controlled by an ‘internal ribosomal entry site’ (IRES) located within the non-coding 5'' region [2]. Only one serotype of HAV has been identified so far. Analysis of HAV genome sequences has revealed 6 genotypes to date [3], with genotype I predominating worldwide. Genotypes IV–VI have only been observed in Old World monkeys. ...

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“…Heat treatment of lyophilized products (e.g. 80 °C for 72 h or 100 °C for at least 30 min) inactivates HIV, provided there is appropriate residual moisture of about 1% [198,199,200]. …”

Section: Blood Productsmentioning

confidence: 99%