Improvement of versatile peroxidase activity and stability by a cholinium-based ionic liquid

Mamashli, Fatemeh; Badraghi, Jalil; Delavari, Behdad; Lanjanian, Hossein; Sabbaghian, Marjan; Hosseini, Morteza; Saboury, Ali Akbar

doi:10.1016/j.molliq.2018.09.128

Cited by 29 publications

(24 citation statements)

References 62 publications

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“…Corresponding controls containing the applied alginate concentrations were prepared to correct for the buffer. Furthermore, CDNN software was applied to deconvolute the data and obtain the contents of various secondary structure elements 30 , 31 .…”

Section: Methodsmentioning

confidence: 99%

Designing a new alginate-fibrinogen biomaterial composite hydrogel for wound healing

Soleimanpour

Mirhaji

Jafari

et al. 2022

Sci Rep

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Wound healing is a complex process and rapid healing necessitates a proper micro-environment. Therefore, design and fabrication of an efficacious wound dressing is an impressive innovation in the field of wound healing. The fabricated wound dressing in this scenario was designed using a combination of the appropriate coagulating and anti-bacterial materials like fibrinogen (as coagulating agent), nisin (as anti-bacterial agent), ethylenediaminetetraacetic acid (as anti-bacterial agent), and alginate (as wound healing agent). Biophysical characterization showed that the interaction of fibrinogen and alginate was associated with minor changes in the secondary structure of the protein. Conformational studies showed that the protein was structurally stable at 42 °C, is the maximum temperature of the infected wound. The properties of the hydrogel such as swelling, mechanical resistance, nisin release, antibacterial activity, cytotoxicity, gel porosity, and blood coagulation were assessed. The results showed a slow release for the nisin during 48 h. Antibacterial studies showed an inhibitory effect on the growth of Gram-negative and Gram-positive bacteria. The hydrogel was also capable to absorb a considerable amount of water and provide oxygenation as well as incorporation of the drug into its structure due to its sufficient porosity. Scanning electron microscopy showed pore sizes of about 14–198 µm in the hydrogel. Cell viability studies indicated high biocompatibility of the hydrogel. Blood coagulation test also confirmed the effectiveness of the synthesized hydrogel in accelerating the process of blood clot formation. In vivo studies showed higher rates of wound healing, re-epithelialization, and collagen deposition. According to the findings from in vitro as well as in vivo studies, the designed hydrogel can be considered as a novel attractive wound dressing after further prerequisite assessments.

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Section: Methodsmentioning

confidence: 99%

Designing a new alginate-fibrinogen biomaterial composite hydrogel for wound healing

Soleimanpour

Mirhaji

Jafari

et al. 2022

Sci Rep

Self Cite

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“…[ 144 ] Recently, ionic liquids have shown to interact with proteins, [ 145 , 146 , 147 ] including enzymes, [ 148 , 149 , 150 , 151 ] with ammonium‐based ionic liquids in particular offering low toxicity and thermal and conformational stability. [ 152 , 153 , 154 ] Although the interactions between proteins and other biological materials and ionic liquids are not yet perfectly understood, great progress has been made in this arena by uniting experimental and theoretical approaches. [ 155 , 156 , 157 , 158 , 159 ] This subsection will describe the current state of knowledge of protein–ionic liquid interactions and how that knowledge has been usefully employed in applications.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

“…A majority of the papers reviewed in this section utilize either E. coli , [ 157 , 160 , 161 , 170 , 190 ] Staphylococcus aureus , [ 184 , 192 ] or both [ 153 , 154 , 155 , 156 , 158 , 162 , 165 , 166 , 168 , 169 , 171 , 173 , 174 , 176 , 183 , 184 ] to test IL/formulation bactericidal efficacy. The two bacterial strains are extremely clinically relevant, as these are both common bacterial infections in humans, and they E. coli and S. aureus represent two general categories of bacteria, Gram‐negative and Gram‐positive, respectively.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

“…In the case of versatile peroxidase, this decreased thermal degradation and improved activity. [ 154 ] The juxtaposition of the two previously mentioned papers highlights the importance of optimizing IL chemistry for the prevention of unwanted protein modifications and a better understanding of the microscopic protein‐IL interactions. A multitude of techniques need to be utilized for a more holistic understanding of protein stability.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

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Recent Advances in Ionic Liquids in Biomedicine

2021

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The use of ionic liquids and deep eutectic solvents in biomedical applications has grown dramatically in recent years due to their unique properties and their inherent tunability. This review will introduce ionic liquids and deep eutectics and discuss their biomedical applications, namely solubilization of drugs, creation of active pharmaceutical ingredients, delivery of pharmaceuticals through biological barriers, stabilization of proteins and other nucleic acids, antibacterial agents, and development of new biosensors. Current challenges and future outlooks are discussed, including biocompatibility, the potential impact of the presence of impurities, and the importance of understanding the microscopic interactions in ionic liquids in order to design task-specific solvents.

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“…It has potential industrial applications for which an alternative to organic solvents would be desirable. Thus, a deep eutectic solvent composed of choline chloride and glycerol (1:2) was tested (Mamashli et al, 2018). The activity of the enzyme at neutral pH was higher in the solvent-buffer mixtures than in buffer alone.…”

Section: The Influence Of Choline-based Ionic Liquids On the Catalytic Activity Of Lipase Frommentioning

confidence: 99%

Deep Eutectic Solvents for Innovative Pharmaceutical Formulations

Nguyen

Augis

Fourmentin

et al. 2020

Environmental Chemistry for a Sustainable World

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Finding alternative solvents for industrial processes, such as chemical synthesis or extraction of biologically active molecules that are less toxic and more environmentally friendly than the organic solvents used up to now, is a major societal issue. These alternative solvents are often described as "green, biodegradable" solvents. Deep eutectic solvents, which are mixtures of simple and often naturally occurring compounds, have been extensively studied in this regard. Among their possible applications, there has been increasing interest in their use for the preparation of pharmaceutical formulations. Indeed, by changing the nature and ratio of their components, deep eutectic solvents can be adapted to a wide range of active molecules, from poorly soluble small molecules to labile macromolecules. The use of deep eutectic solvents to solubilize active molecules with low aqueous solubility and/or low permeability could be an alternative approach to increase their dissolution and in-vivo absorption. This could result in significant increases of bioavailability or enhanced therapeutic efficacy of currently marketed drugs. Moreover, deep eutectic solvents can be used to limit phenomena like polymorphism or degradation which present a challenge to drug formulation. However, despite being generally described as biodegradable and nontoxic due to the nature of their constituents, the safety of deep eutectic solvents, which possess both novel physico-chemical and biological properties, cannot be taken for granted and must therefore be carefully studied during development stages. Therefore, this chapter presents not only recent progress in the application of deep eutectic solvents in the development of formulations for improving therapeutic efficacy by different routes of administration but also studies that have been undertaken to investigate the toxicity of deep eutectic solvents to both living organisms and the environment.

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Improvement of versatile peroxidase activity and stability by a cholinium-based ionic liquid

Cited by 29 publications

References 62 publications

Designing a new alginate-fibrinogen biomaterial composite hydrogel for wound healing

Designing a new alginate-fibrinogen biomaterial composite hydrogel for wound healing

Recent Advances in Ionic Liquids in Biomedicine

Deep Eutectic Solvents for Innovative Pharmaceutical Formulations

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