2019
DOI: 10.3389/fchem.2019.00797
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Improvement of Tryptophan Analysis by Liquid Chromatography-Single Quadrupole Mass Spectrometry Through the Evaluation of Multiple Parameters

Abstract: Tryptophan is a key component in many biological processes and an essential amino acid in food and feed materials. Analysis of the tryptophan content in proteins or protein-containing matrices has always been a challenge. We show here that the preparation of samples prior to tryptophan analysis can be significantly simplified, and the time consumption reduced, by using ascorbic acid as antioxidant to eliminate the problem of tryptophan degradation during alkaline hydrolysis. Combined with separation by HPLC an… Show more

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Cited by 26 publications
(12 citation statements)
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“…The problem of tryptophan instability may be overcome by applying enzymatic hydrolysis or basic hydrolysis with barium hydroxide . A very recent method uses 4 M lithium hydroxide with the addition of ascorbic acid as an antioxidant for optimal tryptophan recovery . However, the conditions that lead to optimal recovery of tryptophan are not necessarily the same that have to be used for optimal recovery of tryptophan oxidation products.…”
Section: Determination Of Individual Products Of Protein Oxidation In...mentioning
confidence: 99%
“…The problem of tryptophan instability may be overcome by applying enzymatic hydrolysis or basic hydrolysis with barium hydroxide . A very recent method uses 4 M lithium hydroxide with the addition of ascorbic acid as an antioxidant for optimal tryptophan recovery . However, the conditions that lead to optimal recovery of tryptophan are not necessarily the same that have to be used for optimal recovery of tryptophan oxidation products.…”
Section: Determination Of Individual Products Of Protein Oxidation In...mentioning
confidence: 99%
“…Furthermore, Delgado-Andrade et al [42] proposed a liquid chromatography-based method with fluorescence detection for TRP analysis in milk-based ingredients, but the total chromatographic time, even if not specified by the authors in the text, was at least 10 min. The running chromatographic time of this proposed method was even shorter than that proposed by La Cour et al [43] with UHPLC-single quadrupole mass spectrometry, who reported a total analysis time of 11.5 min for TRP analysis in plant materials and dog foods, even if the author stated a possibility of shortening the running time.…”
Section: Methods Validationmentioning
confidence: 70%
“…The determination of tryptophan in samples was performed according to la Cour et al . 25 . All amino acids were analysed by HPLC (Agilent 1100, Agilent Technologies, Avondale, PA, USA), combined with automated pre-column derivatisation using o-phthaldialdehyde and 9-fluorenylmethyl chloroformate, as reported by Henderson et al .…”
Section: Methodsmentioning
confidence: 99%