Improvement of the systemic prime/oral boost strategy for systemic and local responses

Lauterslager, Tosca G.M; Stok, W.; Hilgers, Luuk A.Th.

doi:10.1016/s0264-410x(02)00687-4

Cited by 27 publications

(19 citation statements)

References 23 publications

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“…DNA boost, the oral F4 boost was clearly more immunogenic for the adjuvanted groups, as judged by an enhanced isotype switching towards IgG in the spleen and increased numbers of IgG ASC in the Cerv LN. This is in agreement with other studies demonstrating that prime-boost immunizations by alternating routes and/or immunization forms are as immunogenic or more immunogenic than homologous prime-boost immunizations (Baca-Estrada et al, 2000;McCluskie et al, 2002;Lauterslager et al, 2003). As observed previously (Melkebeek et al, 2007), boosting of systemic responses in parenterally primed pigs by oral F4 administration appeared to be independent of the F4R status of the pigs.…”

Section: Discussionsupporting

confidence: 92%

Comparison of immune responses in parenteral FaeG DNA primed pigs boosted orally with F4 protein or reimmunized with the DNA vaccine

Melkebeek

Verdonck

Goddeeris

et al. 2007

Veterinary Immunology and Immunopathology

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We previously showed that an intradermal (i.d.) FaeG DNA prime (2Â)-oral F4 protein boost immunization induces a systemic response and weakly primes a mucosal IgG response in pigs, especially when plasmid vectors encoding the A and B subunit of the E. coli thermo-labile enterotoxin (LT) are added to the DNA vaccine. In the present study, we evaluated whether addition of 1a,25-dihydroxyvitamin D 3 (vitD 3 ) to the DNA vaccine could further enhance this mucosal priming and/or modulate the antibody response towards IgA. To further clarify priming of systemic and mucosal responses by the i.d. DNA vaccination, we firstly compared the localization of the F4-specific antibody response in pigs that were orally boosted with F4 to that in pigs that received a third i.d. DNA immunization and secondly evaluated cytokine mRNA expression profiles after i.d. DNA vaccination. The i.d. DNA prime (2Â)-oral F4 boost immunization as well as the 3 i.d. DNA vaccinations induced mainly a systemic response, with a higher response observed following the heterologous protocol. Co-administration of vitD 3 , and especially of the LT vectors, enhanced this response. Furthermore, only the heterologous immunization resulted in a weak mucosal priming, which appeared to require the presence of the LT vectors or vitD 3 as adjuvants. In addition, the LT vectors strongly enhanced the FaeG-specific lymphocyte proliferation and this was accompanied by the absence of a clear IL-10 response. However, despite two DNA immunizations in the presence of these adjuvants and an oral F4 boost, we failed to demonstrate the secretory IgA response needed to be protective against enterotoxigenic E. coli. #

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Section: Discussionsupporting

confidence: 92%

Comparison of immune responses in parenteral FaeG DNA primed pigs boosted orally with F4 protein or reimmunized with the DNA vaccine

Melkebeek

Verdonck

Goddeeris

et al. 2007

Veterinary Immunology and Immunopathology

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“…The results of the above-described experiment showed that the FI-BRSVCpG-PP formulation was most effective compared to FI-BRSV-CpG and FI-BRSV-PP after two vaccinations. There is evidence that combinations of mucosal and parenteral administration result in immune responses that are as strong as or stronger than those resulting from either mucosal or parenteral immunization alone (21,22,29,30), so to determine the optimal route of delivery in terms of induction of immunity and protection from BRSV, mice were immunized with FI-BRSV, formulated with both CpG ODN and PP, using different strategies. Increased IgG titers were observed in all vaccinated groups compared to the mock-vaccinated control groups (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…delivered FI-BRSV vaccine in mice (32). Since there is evidence that combinations of mucosal and parenteral administration lead to immune responses that were as strong or stronger than those resulting from either mucosal or parenteral immunization alone (21,22,29,30), the purpose of this study was to determine the effects of the route of delivery on antibody, mucosal, and cell-mediated immune responses, as well as protection against BRSV. We compared i.n.…”

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confidence: 99%

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Intranasal Immunization of Mice with a Bovine Respiratory Syncytial Virus Vaccine Induces Superior Immunity and Protection Compared to Those by Subcutaneous Delivery or Combinations of Intranasal and Subcutaneous Prime-Boost Strategies

Mapletoft¹,

Latimer²,

Babiuk

et al. 2010

Clin Vaccine Immunol

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Bovine respiratory syncytial virus (BRSV) infects cells of the respiratory mucosa, so it is desirable to develop a vaccination strategy that induces mucosal immunity. To achieve this, various delivery routes were compared for formalin-inactivated (FI) BRSV formulated with CpG oligodeoxynucleotide (ODN) and polyphosphazene (PP). Intranasal delivery of the FI-BRSV formulation was superior to subcutaneous delivery in terms of antibody, cell-mediated, and mucosal immune responses, as well as reduction in virus replication after BRSV challenge. Although intranasal delivery of FI-BRSV also induced higher serum and lung antibody titers and gamma interferon (IFN-␥) production in the lungs than intranasal-subcutaneous and/or subcutaneous-intranasal prime-boost strategies, no significant differences were observed in cell-mediated immune responses or virus replication in the lungs of challenged mice. Interleukin 5 (IL-5), eotaxin, and eosinophilia were enhanced after BRSV challenge in the lungs of subcutaneously immunized mice compared to unvaccinated mice, but not in the lungs of mice immunized intranasally or through combinations of the intranasal and subcutaneous routes. These results suggest that two intranasal immunizations with FI-BRSV formulated with CpG ODN and PP are effective and safe as an approach to induce systemic and mucosal responses, as well to reduce virus replication after BRSV challenge. Furthermore, intranasal-subcutaneous and subcutaneous-intranasal primeboost strategies were also safe and almost as efficacious. In addition to the implications for the development of a protective BRSV vaccine for cattle, formulation with CpG ODN and PP could also prove important in the development of a mucosal vaccine that induces protective immunity against human RSV.

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“…Other studies show that oral vaccination is an effective approach to immunize fish by the encapsulation of pDNA [11,12]. Oral vaccination is an attractive way to induce immunity [13], and antigen-specific antibodies in the skin mucus, bile or intestine in several fish species were resulted in by oral administration [14]. Activation of mucosal immunity appears to be an important factor, since many pathogens enter the fish through mucosal surfaces [15].…”

Section: Introductionmentioning

confidence: 99%

The formulation and immunisation of oral poly(DL-lactide-co-glycolide) microcapsules containing a plasmid vaccine against lymphocystis disease virus in Japanese flounder (Paralichthys olivaceus)

Tian

Sun

Chen

et al. 2008

International Immunopharmacology

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Improvement of the systemic prime/oral boost strategy for systemic and local responses

Cited by 27 publications

References 23 publications

Comparison of immune responses in parenteral FaeG DNA primed pigs boosted orally with F4 protein or reimmunized with the DNA vaccine

Comparison of immune responses in parenteral FaeG DNA primed pigs boosted orally with F4 protein or reimmunized with the DNA vaccine

Intranasal Immunization of Mice with a Bovine Respiratory Syncytial Virus Vaccine Induces Superior Immunity and Protection Compared to Those by Subcutaneous Delivery or Combinations of Intranasal and Subcutaneous Prime-Boost Strategies

The formulation and immunisation of oral poly(DL-lactide-co-glycolide) microcapsules containing a plasmid vaccine against lymphocystis disease virus in Japanese flounder (Paralichthys olivaceus)

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