Improvement of the Survival of Human Autologous Fat Transplantation by Using VEGF-Transfected Adipose-Derived Stem Cells

Lu, Feng; Li, Jie; Gao, Jianhua; Ogawa, Rei; Chen, Ou; Yang, Bo; Bing-chuan, FU

doi:10.1097/prs.0b013e3181babbb6

Cited by 194 publications

(161 citation statements)

References 28 publications

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“…However, the clinical successes are greatly suppressed by the low survival ratio due to fat absorption and fibrosis (Lu et al 2009). Previous studies have demonstrated that normal fat transplantation possesses a tissue reduction of 40-60% (Peer 1950;Chajchir et al 1990).…”

Section: Discussionmentioning

confidence: 99%

“…Fat transplantation has shown great potential in plastic surgery and tissue repair due to its simple procedure and minimal donor-site morbidity (Xu et al 2007;Lu et al 2009). However, the clinical successes are greatly suppressed by the low survival ratio due to fat absorption and fibrosis (Lu et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The survival ratio of the harvested tissues was calculated by using method as previously published (Lu et al 2009). In brief, the harvested tissue at each condition was suspended in 0.2 mL PBS in graduated containers.…”

Section: In Vivo Fat Transplantationmentioning

confidence: 99%

“…Furthermore, AFT has been considered as a promising alternative to artificial tissue implants Knippenberg et al 2005). However, a low survival ratio and subsequent side effects, such as necrosis and calcification, result in the unpredictable degree of absorption which limits its clinical efficacy and further applications (Chajchir et al 1990;Lu et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Compared with bone marrow mesenchymal stem cells (MSCs), which have been extensively studied for stem cellbased therapies, the use of ADSCs in tissue repair and regeneration is further encouraged by its abundant existence in the human body and its easy-availability with negligible donor-site morbidity and patient discomfort (Xu et al 2007;Lu et al 2009). However, ADSCs exhibit lower proliferation and differentiation activities compared to MSCs (De Ugarte et al 2003;Huang et al 2005), requiring further improvement in this area.…”

Section: Introductionmentioning

confidence: 99%

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Adipose Tissue-Derived Stem Cells Treated with Estradiol Enhance Survival of Autologous Fat Transplants

Luo

Hao

et al. 2013

Tohoku J. Exp. Med.

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Autologous fat transplantation (AFT) is a common and important operation in plastic surgery for soft tissue defects and adipose tissue-derived stem cells (ADSCs) are considered as a promising supplement to decrease absorption and subsequent side effects due to the ability of multiple differentiation and production of vascular endothelial growth factor (VEGF). The capacities of ADSCs can be further enhanced by treatment with 17-β estradiol (E2). Therefore, we hypothesized that E2 may promote the potential of ADSCs for AFT. In this study, ADSCs were extracted from three female patients by liposuction. In vitro studies showed that E2 supplementation at an optimal concentration of 10 -8 M resulted in enhanced proliferation, VEGF production, and adipogenic differentiation of human ADSCs, and reduced apoptosis rate in a serum-free environment. In addition, a nude mice model of fat transplantation was utilized to demonstrate the efficacy of ADSC for survival ratio in vivo. These results using the volume of fat tissues after 12 weeks compared original volume, revealed that the addition of E2-treated ADSCs induced a significantly higher tissue survival ratio (76.9 ± 1.9%) when compared with the ADSC-free system (55.5 ± 1.5%). Furthermore, increased capillary formation stained with hematoxylin-eosin (H&E) was observed in ADSCs systems after treatment with E2. Therefore, this study demonstrated E2 could promote the capacities of ADSCs about aspects of adipogenic differentiation, growth factor secretion and apoptosis reduction in vitro, vascularization improvement in vivo, and then enhanced the survival ratio of AFT.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: In Vivo Fat Transplantationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Adipose Tissue-Derived Stem Cells Treated with Estradiol Enhance Survival of Autologous Fat Transplants

Luo

Hao

et al. 2013

Tohoku J. Exp. Med.

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Injectable Mussel‐Inspired Immobilization of Platelet‐Rich Plasma on Microspheres Bridging Adipose Micro‐Tissues to Improve Autologous Fat Transplantation by Controlling Release of PDGF and VEGF, Angiogenesis, Stem Cell Migration

Zhou

Chang

et al. 2017

Adv Healthcare Materials

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Platelets-rich plasma (PRP) can produce growth factors (GFs) to improve angiogenesis. However, direct injection of PRP does not lead to highly localized GFs. The current study employs a mussel-inspired polydopamine to immobilize PRP on gelatin microspheres (GMs) with the purpose of bridging adipose micro-tissues to help implanted fat survive (GM-pDA-PRP). Enhanced PRP adhesion leads to a prolonged and localized production of GFs, which is verified by platelet counting and by ELISA of vascular endothelial growth factors (VEGFs) and of platelet derived growth factors (PDGFs). The GM-pDA-PRP "hatches" a microenvironment for the proliferation of adipose-derived stem cells. After the adipose micro-tissue has bridged with GM-pDA-PRP after 16 weeks, triple-fluorescence staining reveals that the mature adipocytes, blood vessels, and capillaries are arranged like in normal adipose tissue. The survival fat increases significantly compared to that in control, PRP, and GM-PRP groups (84.8 ± 11.4% versus 47.8 ± 8.9%, 56.9 ± 9.7%, and 60.2 ± 10.5%, respectively). Both histological assessments and CD31 immunofluorescence indicate that the improvement of angiogenesis in GM-pDA-PRP is higher than in the fat graft group (6.4-fold in quantitative CD31 positive cells). The CD34 positive cells in the GM-pDA-PRP group are around 3.5-fold the amount in the fat graft group, which suggests that more stem cells migrate to the implant area. Cell proliferation staining shows that the number of Ki67 positive cells is around five times as high as that in the fat graft group.

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Role of adipose‐derived stem cells in enhancing angiogenesis early after aspirated fat transplantation: induction or differentiation?

Yuan

Gao

Liu

et al. 2013

Cell Biology International

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Autologous fat tissue has been used as a potential filler for soft-tissue defects, despite unpredictable clinical outcomes and low graft survival. Co-transplantation of adipose-derived stem cells (ASCs) is an alternative therapeutic approach to effectively enhance the survival and quality of transplanted fat tissue by increasing neovascularization. Nevertheless, the mechanisms by which ASCs exerted their angiogenic effects remain obscure. ASCs can secrete several angiogenic growth factors, for example vascular endothelial growth factor, hepatocyte growth factor and basic fibroblast growth factor. Hypoxic conditions may promote the proliferation of ASCs and their secretion. However, the differentiation of ASCs into endothelial cells (ECs), pericytes and smooth muscular cells in vivo has not been confirmed. The role of ASCs early after aspirated fat transplantation may be to induce new vessels from the recipient region to grow around and into the graft by releasing significant amounts of angiogenic growth factors rather than to differentiate into ECs, pericytes or smooth muscular cells forming new vessels, an effect that might be enhanced by hypoxia.

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Improvement of the Survival of Human Autologous Fat Transplantation by Using VEGF-Transfected Adipose-Derived Stem Cells

Abstract: Adipose-derived stem cells together with VEGF transduction can enhance the survival and quality of transplanted fat tissues.

Cited by 194 publications

References 28 publications

Adipose Tissue-Derived Stem Cells Treated with Estradiol Enhance Survival of Autologous Fat Transplants

Adipose Tissue-Derived Stem Cells Treated with Estradiol Enhance Survival of Autologous Fat Transplants

Injectable Mussel‐Inspired Immobilization of Platelet‐Rich Plasma on Microspheres Bridging Adipose Micro‐Tissues to Improve Autologous Fat Transplantation by Controlling Release of PDGF and VEGF, Angiogenesis, Stem Cell Migration

Role of adipose‐derived stem cells in enhancing angiogenesis early after aspirated fat transplantation: induction or differentiation?

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