Improvement of the Production and Characterisation of Polyhydroxyalkanoate by Bacillus endophyticus Using Inexpensive Carbon Feedstock

Geethu, M.; Vrundha, R.; Raja, S.; Chandrashekar, H. Raghu; Divyashree, M S

doi:10.1007/s10924-019-01397-z

Cited by 29 publications

(6 citation statements)

References 39 publications

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“…A similar statistical study was conducted previously under controlled medium condition with initial 4 % sugar content provided by SCM at 250 rpm by test organism which gave upto 68.5 % of PHA. This result was published and was also found that PHA accumulation started during the late log phase utilising the sugar content as expected and gave a maximum production of 5.85 g/L from 8.53 g/L of biomass at 72 h (Geethu et al 2019).…”

Section: Bioreactor Studies For the Optimised Mediumsupporting

confidence: 78%

“…In our another study it was reported that PHA production in the presence of inexpensive carbon source like sugarcane molasses (4 %) with increased agitation rate enhanced PHA production by 10.7 g/L with 15.37 g/L of cell mass statistically. This experimental model created using CCD reported that increase in sugar concentration (> 4%) had negative influence on biopolymer production (Geethu et al 2019).…”

Section: Interaction Of Variables On Responsesmentioning

confidence: 99%

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Statistical Optimisation of Polyhydroxyalkanoate Production in Bacillus Endophyticus Using Sucrose as Sole Source of Carbon

Geethu

Hariharapura²,

Divyashree

2021

Preprint

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Microorganisms have been contemplated as a promising source for the inexhaustible synthesis of many novel materials utilizing renewable sources. Among many of such products, Polyhydroxyalkanoate (PHA) remains as an essential biodegradable polymer with functions similar to conventional plastics. Bacillus endophyticus is capable of accumulating biopolymer PHA in nutrient limiting conditions with excess of carbon source. Screening and optimizing the parameters for increased PHA production was done statistically. The optimized medium gave a maximum yield of 46.57 % which was in well agreement with the given predicted value provided by Response surface Methodology model yield of 47.02 %. Optimal media conditions when extrapolated in bioreactor gave an even higher production percentage of 49.9.This is the first report highlighting 49 % of Polyhydroxybutyrate statistically using sucrose as a source. The main highlight of the study was the use of wild type strain for producing high quality PHA using simple carbon source which can be a starting platform for using this strain for large scale PHA production industrially. FTIR and 1HNMR analysis confirmed the polymer produced.

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Section: Bioreactor Studies For the Optimised Mediumsupporting

confidence: 78%

Section: Interaction Of Variables On Responsesmentioning

confidence: 99%

Statistical Optimisation of Polyhydroxyalkanoate Production in Bacillus Endophyticus Using Sucrose as Sole Source of Carbon

Geethu

Hariharapura²,

Divyashree

2021

Preprint

View full text Add to dashboard Cite

show abstract

“…A similar statistical study was conducted previously under controlled medium condition with initial 4% sugar content provided by SCM at 250 rpm by test organism which gave upto 68.5% of PHA. This result was published and was also found that PHA accumulation started during the late log phase utilising the sugar content as expected and gave a maximum production of 5.85 g/L from 8.53 g/L of biomass at 72 h (Geethu et al 2019). The 1 h interval time course growth study of B. endophyticus during growth in the bioreactor revealed that the organism directly utilise sucrose and produce PHA during the growth phase (Fig.…”

Section: Interaction Of Variables On Responsessupporting

confidence: 78%

“…In our another study, it was reported that PHA production in the presence of inexpensive carbon source like sugarcane molasses (4%) with increased agitation rate enhanced PHA production by 10.7 g/L with 15.37 g/L of cell mass statistically. This experimental model created using CCD reported that increase in sugar concentration (> 4%) had a negative influence on biopolymer production (Geethu et al 2019). Mohanrasu (2020) reported the importance of nitrogen, potassium and sodium concentration in the basal medium for cell growth for the PHA producers which later on limiting (Mohanrasu et al 2020).…”

Section: Interaction Of Variables On Responsesmentioning

confidence: 99%

Statistical optimisation of polyhydroxyalkanoate production in Bacillus endophyticus using sucrose as sole source of carbon

Geethu

Chandrashekar

2021

Arch Microbiol

Self Cite

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Microorganisms have been contemplated as a promising source for the inexhaustible synthesis of many novel materials utilizing renewable sources. Among many of such products, polyhydroxyalkanoate (PHA) remains as an essential biodegradable polymer with functions similar to conventional plastics. Bacillus endophyticus is capable of accumulating biopolymer PHA in nutrient limiting conditions with excess of carbon source. Screening and optimizing the parameters for increased PHA production was done statistically. The optimized medium gave a maximum yield of 46.57% which was in well agreement with the given predicted value provided by response surface methodology model yield of 47.02%. Optimal media conditions when extrapolated in bioreactor gave an even higher production percentage of 49.9. This is the first report highlighting 49% of polyhydroxybutyrate statistically using sucrose as a source. The main highlight of the study was the use of wild type strain for producing high quality PHA using simple carbon source which can be a starting platform for using this strain for large scale PHA production industrially. FTIR and 1HNMR analysis confirmed the polymer produced.

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“…However, agitation rates below the optimal level caused cell aggregation and a heterogeneous culture media (Musa et al, 2016). Furthermore, agitation was used as an optimisation parameter of PHA production in several studies (Aramvash et al, 2015;Geethu et al, 2019). Increasing the concentration of 1-pentanol from 0.03 to 0.09 wt% considerably increased the 3HV monomer composition, but the latter was unaffected by agitation or incubation time (Fig.…”

Section: Optimization Of P(3hb-co-3hv) Production Using the Statisticmentioning

confidence: 99%

Optimizing the biosynthesis of renewable polyhydroxyalkanoate copolymer containing 3-hydroxyvalerate by Massilia haematophila using statistical modeling

Kiun¹,

Amelia²,

Huong³

et al. 2019

bta

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Polyhydroxyalkanoate (PHA) is a microbial storage polymer that is naturally produced by certain bacteria. This is the first study on the ability of this particular species Massilia haematophila to synthesize a PHA copolymer containing 3-hydroxyvalerate (3HV) monomer. Using the statistical design on Massilia haematophila UMTKB-2, this study highlights the optimization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), copolymer production for shaken-flask cultivation. Moreover, the mechanical and thermal features of the polymers were determined. The production of P(3HB-co-3HV) by Massilia haematophila UMTKB-2 using optimal conditions provided by response surface methodology (RSM) yielded 5.0 g/l of P(3HB-co-7 mol% 3HV), which was higher than the value obtained from unoptimized conditions such as 4.40 g/l of P(3HB-co-4mol% 3HV). This result showed a 14% increase in copolymer concentration and a twofold increase in 3HV composition. In this study, the P(3HB-co-3HV) synthesized was determined as a block copolymer and its thermal properties were better than P(3HB). Using RSM, the optimization conditions were successfully obtained for this bacterium, and this result is a starting platform for additional studies of a larger scaled PHA production from Massilia haematophila UMTKB-2 using bioreactors.

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Improvement of the Production and Characterisation of Polyhydroxyalkanoate by Bacillus endophyticus Using Inexpensive Carbon Feedstock

Cited by 29 publications

References 39 publications

Statistical Optimisation of Polyhydroxyalkanoate Production in Bacillus Endophyticus Using Sucrose as Sole Source of Carbon

Statistical Optimisation of Polyhydroxyalkanoate Production in Bacillus Endophyticus Using Sucrose as Sole Source of Carbon

Statistical optimisation of polyhydroxyalkanoate production in Bacillus endophyticus using sucrose as sole source of carbon

Optimizing the biosynthesis of renewable polyhydroxyalkanoate copolymer containing 3-hydroxyvalerate by Massilia haematophila using statistical modeling

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