Improvement of the intestinal epithelial barrier during laxative effects of phlorotannin in loperamide-induced constipation of SD rats

Kim, Ji Eun; Song, Hee Jin; Choi, Yun Ju; Jin, You Jeong; Roh, Yu Jeong; Seol, Ayun; Park, So Hae; Park, Ju Min; Kang, Hyun Gu; Hwang, Dae Youn

doi:10.1186/s42826-022-00152-1

Cited by 4 publications

(4 citation statements)

References 49 publications

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“…The proper functioning of the intestines is closely linked to the maintenance of the intestinal epithelial barrier. This barrier can become compromised in chronic constipation [ 34 ]. AS-IV has been shown to improve the dysfunction of the intestinal barrier in different types of diseases [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Single-cell RNA sequencing reveals cellular dynamics and therapeutic effects of astragaloside IV in slow transit constipation

Chen,

Wan,

et al. 2024

Biomol Biomed

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The cellular characteristics of intestinal cells involved in the therapeutic effects of astragaloside IV (AS-IV) for treating slow transit constipation (STC) remain unclear. This study aimed to determine the dynamics of colon tissue cells in the STC model and investigate the effects of AS-IV treatment by single-cell RNA sequencing (scRNA-seq). STC mouse models were developed using loperamide, with subsequent treatment using AS-IV. Colon tissues and feces were collected for scRNA-seq and targeted short-chain fatty acid quantification. We integrated scRNA-seq data with network pharmacology to analyze the effect of AS-IV on constipation. AS-IV showed improvement in defecation for STC mice induced by loperamide. Notably, in STC mice, epithelial cells, T cells, B cells, and fibroblasts demonstrated alterations in cell proportions and dysfunctions, which AS-IV partially rectified. AS-IV has the potential to modulate the metabolic pathway of epithelial cells through its interaction with peroxisome proliferator-activated receptor gamma (Pparγ). AS-IV reinstated fecal butyrate levels and improved energy metabolism in epithelial cells. The proportion of naïve CD4+T cells is elevated in STC, and the differentiation of these cells into Treg is regulated by B cells and fibroblasts through the interaction of ligand-receptor pairs. AS-IV treatment can partially alleviate this trend. The status of fibroblasts in STC undergoes alterations, and the FB_C4_Adamdec1 subset, associated with angiogenesis and the Wingless-related integration (Wnt) pathway, emerges. Our comprehensive analysis identifies perturbations of epithelial cells and tissue microenvironment cells in STC and elucidates mechanisms underlying the therapeutic efficacy of AS-IV.

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Section: Discussionmentioning

confidence: 99%

Single-cell RNA sequencing reveals cellular dynamics and therapeutic effects of astragaloside IV in slow transit constipation

Chen,

Wan,

et al. 2024

Biomol Biomed

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“…There are many other reported wide-ranging health benefits for E. cava extracts or fractions. For example, a review by Kim et al (2023) states that the Korean Ministry of Food and Drug Safety approved the use of an E. cava supplement containing phlorotannin as a health-functional product that helps improve sleep quality. The paper covers the sedative-hypnotic mechanisms in animal models as well as human clinical trials.…”

Section: Colored Corn Floursmentioning

confidence: 99%

“…Li et al [39] found that administering the E. cava component dieckol to the inner ear of rats prevented ototoxic hearing loss, suggesting that the procedure may be a safe and effective method to prevent drug-induced hearing loss. Park et al [40] reported that a water extract of E. cava acts as a potential therapeutic agent against fine dust induced health damage in mice by regulating gut function of the microbiota and Kim et al [41] provides evidence for the use of phlorotannin against constipation in rats.…”

Section: Colored Corn Floursmentioning

confidence: 99%

Composition of Flatbreads Prepared from Blue, Red, Yellow, and White Corn Flours Supplemented with Edible Ecklonia cava Algae: Relationship to Health Benefits

2024

Food Nutr J

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The objective of the present study was to determine the composition of flatbreads prepared from corn and wheat flours supplemented with edible Ecklonia cava, which is a health-promoting medicinal food used as a dietary supplement. The Corn flours used were Whole Blue, Purcell Whole Blue, Purcell Whole Red, Purcell Whole Yellow, Purcell Whole White and Giant White and King Arthur Whole Wheat. Because the colored corn flours are reported to contain health-promoting anthocyanins and the Ecklonia alga contains multiple biologically active polyphenolic and other health-promoting compounds reported to show anti-asthma, anti-cancer, anti-diabetes, antimicrobial, anti-neurotoxicity, anti-obesity, and cardioprotective effects, the resulting flatbreads have the potential for multiple health benefits in humans after consumption. To facilitate the development of the newly created flatbreads for consumption, we determined, using standard methods, the effect of added E. cava on the proximate composition of protein, fat, carbohydrate, dry matter, ash (mineral), and water content of the flatbreads. The unexpected results, for which we have no explanation, show that the measured protein, mineral, and water content of the E. cava-supplemented flatbreads increased, whereas the fat, carbohydrate, and dry matter contents of the E. cava-supplemented flatbreads decreased compared to un-supplemented ones, suggesting that consumers will be able to select flatbreads with a high-protein and low-fat content.

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“…2023, 24, 15757 2 of 24 chemicals and diet, were widely applied to produce animal models with constipation phenotypes. In most cases, the human-like constipation was successfully induced by the oral administration or subcutaneous injection of loperamide (Lop) hydrochloride as well as by the feeding of a low-fiber diet containing 41.5% cornstarch, 24.5% milk casein, 10.0% sucrose, 10.0% dextrin, 7.0% mineral mixture, 6.0% corn oil, and 1.0% vitamin mixture for 5 weeks [8][9][10][11]. Also, the phenotypes for constipation could be induced by treatment with opioids and carbon, although these methods are rarely used [12,13].…”

Section: Introductionmentioning

confidence: 99%

Complement C3-Deficiency-Induced Constipation in FVB/N-C3em1Hlee/Korl Knockout Mice Was Significantly Relieved by Uridine and Liriope platyphylla L. Extracts

Song,

Kim,

Jin

et al. 2023

IJMS

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Complement component 3 (C3) deficiency has recently been known as a cause of constipation, without studies on the therapeutic efficacy. To evaluate the therapeutic agents against C3-deficiency-induced constipation, improvements in the constipation-related parameters and the associated molecular mechanisms were examined in FVB/N-C3em1Hlee/Korl knockout (C3 KO) mice treated with uridine (Urd) and the aqueous extract of Liriope platyphylla L. (AEtLP) with laxative activity. The stool parameters and gastrointestinal (GI) transit were increased in Urd- and AEtLP-treated C3 KO mice compared with the vehicle (Veh)-treated C3 KO mice. Urd and AEtLP treatment improved the histological structure, junctional complexes of the intestinal epithelial barrier (IEB), mucin secretion ability, and water retention capacity. Also, an improvement in the composition of neuronal cells, the regulation of excitatory function mediated via the 5-hydroxytryptamine (5-HT) receptors and muscarinic acetylcholine receptors (mAChRs), and the regulation of the inhibitory function mediated via the neuronal nitric oxide synthase (nNOS) and inducible NOS (iNOS) were detected in the enteric nervous system (ENS) of Urd- and AEtLP-treated C3 KO mice. Therefore, the results of the present study suggest that C3-deficiency-induced constipation can improve with treatment with Urd and AEtLP via the regulation of the mucin secretion ability, water retention capacity, and ENS function.

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Improvement of the intestinal epithelial barrier during laxative effects of phlorotannin in loperamide-induced constipation of SD rats

Cited by 4 publications

References 49 publications

Single-cell RNA sequencing reveals cellular dynamics and therapeutic effects of astragaloside IV in slow transit constipation

Single-cell RNA sequencing reveals cellular dynamics and therapeutic effects of astragaloside IV in slow transit constipation

Composition of Flatbreads Prepared from Blue, Red, Yellow, and White Corn Flours Supplemented with Edible Ecklonia cava Algae: Relationship to Health Benefits

Complement C3-Deficiency-Induced Constipation in FVB/N-C3em1Hlee/Korl Knockout Mice Was Significantly Relieved by Uridine and Liriope platyphylla L. Extracts

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