Elaboration of methods for the control of biochemical reactions with deoxyribonucleic acid (DNA) strands is necessary for the solution of one of the basic problems in the creation of biocomputers-improvement in the reliability of molecular DNA computing. In this paper, the results of the solution of the four-parameter inverse problem of laser Raman spectroscopythe determination of the type and concentration of each of the DNA nitrogenous bases in multi-component solutions-are presented.