Improvement of techniques for mass-culture of Chilo partellus (Swinhoe)

Ochieng, R. S.; Onyango, Fredrick; Bungu, M. D. O.

doi:10.1017/s1742758400004744

Cited by 70 publications

(65 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A culture of Chilo partellus was initiated from field collected stemborers and reared on a semi-synthetic diet developed by Ochieng et al (1985). The parasitoid Cotesia sesamiae Cameron (Hymenoptera: Braconidae) was reared on stemborer larvae according to the method described by Overholt et al (1994).…”

Section: Methodsmentioning

confidence: 99%

Oviposition Induced Volatile Emissions from African Smallholder Farmers’ Maize Varieties

et al. 2012

View full text Add to dashboard Cite

Maize (corn), Zea mays, is a genetically diverse crop, and we have recently shown that certain open pollinated varieties (OPVs) of Latin American origin possess a trait not present in mainstream commercial varieties: they produce volatiles in response to stemborer oviposition that are attractive to stemborer parasitoids. Here, we tested whether a similar tritrophic effect occurs in the African OPVs 'Nyamula' and 'Jowi'. Herbivore induced plant volatiles (HIPVs) were collected from plants exposed to egg deposition by the stemborer Chilo partellus. In a four-arm olfactometer bioassay, the parasitic wasp Cotesia sesamiae preferred samples containing HIPVs from plants with eggs to samples collected from plants without eggs. EAG-active compounds, including (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), were released in higher amounts from the egg induced headspace samples. Our results suggest that this oviposition trait is not limited to S. American Z. mays germplasm, and that it could be used to increase indirect defense against attack by stemborers.

show abstract

Section: Methodsmentioning

confidence: 99%

Oviposition Induced Volatile Emissions from African Smallholder Farmers’ Maize Varieties

et al. 2012

View full text Add to dashboard Cite

show abstract

“…C. flavipes was reared on fourth instar larvae of Chilo partellus using the "hand-stinging" method described in Overholt et al (1994). C. partellus larvae were maintained on artificial diet (Ochieng et al, 1985) at 25Ϯ2°C, 70-80% RH and 12:12 (L:D) h photoperiod.…”

Section: Methodsmentioning

confidence: 99%

“…We used a standard host size in these experiments because C. flavipes lays more eggs in medium-and large-larvae (Ngi-Song et al, 1995). Parasitized larvae were reared on artificial diet (Ochieng et al, 1985) at 25Ϯ2°C, 70-80% RH and 12:12 (L:D) h photoperiod until cocoon formation, a process which took 12-15 days. Cocoon masses were collected in glass vials (2.5 cmϫ7.0 cm) and placed in an incubator at 25°C for adult emergence.…”

Section: Methodsmentioning

confidence: 99%

Cotesia flavipes Cameron (Hymenoptera: Braconidae) does not exhibit complementary sex determination (ii) Evidence from laboratory experiments

Niyibigira

Overholt

Stouthamer³

2004

Appl. Entomol. Zool.

View full text Add to dashboard Cite

Sex determination in the order Hymenoptera is based on haplodiploid arrhenotoky; in which males develop from unfertilized eggs and are haploid, whereas females develop from fertilized eggs and are diploid. However, some hymenopteran species produce diploid males through a mechanism known as single-locus complementary sex determination (sl-CSD). In these species, heterozygous individuals at a single sex locus develop into females, whereas hemizygotes (haploids) and homozygotes (diploids) develop into males. Inbreeding leads to homozygosity and consequent production of diploid males. We investigated the presence of sl-CSD in the braconid Cotesia flavipes using a series of inbreeding crosses among five isofemale lines. Sex ratio (proportion of females) did not differ among the within-line crosses, between-line crosses and crosses carried out between isofemale lines and a mixed (outbred) colony. Brood size of within-line and between-line crosses did not differ. Culturing populations for 25 generations did not result in changes to more male-biased sex ratios. We conclude that CSD does not exist in C. flavipes. The implications of absence of CSD in C. flavipes are discussed in context of mass rearing for classical biological control programmes.

show abstract

“…Eldana saccharina and B. fusca were collected from maize fields in the Western Province, while C. partellus originated from maize grown in the coastal region. The larvae were reared on the artificial diets described by Ochieng et al (1985) (for C. partellus) and Onyango & Ochieng'-Odero (1994) (for B. fusca and E. saccharina). Thrice a year feral stemborer larvae from their respective locations were added to rejuvenate the colonies.…”

Section: Insectsmentioning

confidence: 99%

Host recognition and acceptance behaviour in Cotesia sesamiae and C. flavipes (Hymenoptera: Braconidae), parasitoids of gramineous stemborers in Africa

Calatayud¹,

Rü²,

Obonyo³

et al. 2010

Eur. J. Entomol.

View full text Add to dashboard Cite

Abstract. The host recognition and acceptance behaviour of two braconid larval parasitoids (Cotesia sesamiae and C. flavipes) were studied using natural stemborer hosts (i.e., the noctuid Busseola fusca for C. sesamiae, and the crambid Chilo partellus for C. flavipes) and a non-host (the pyralid Eldana saccharina). A single larva was introduced into an arena together with a female parasitoid and the behaviour of the wasp recorded until it either stung the larva or for a maximum of 5 min if it did not sting the larva. There was a clear hierarchy of behavioural steps, which was similar for both parasitoid species. In the presence of suitable host larvae, after a latency period of 16-17 s, the wasp walked rapidly drumming the surface with its antennae until it located the larva. After location and antennal examination of the host, which lasted 60-70 s and 30 s, respectively, the parasitoid inserted its ovipositor. Stinging that resulted in successful oviposition usually lasted 5-6 s. In the presence of non-host larvae, the latency period was between 25-70 s, and parasitoids spent significantly more time walking and antennal drumming on larvae without ovipositing. It is likely that these two parasitoid species use their antennae for host recognition, and both their antennae and tarsi for final acceptance of a host for oviposition. In both C. sesamiae and C. flavipes tactile and contact-chemoreception stimuli from the hosts seemed to play a major role in the decision to oviposit.

show abstract

Improvement of techniques for mass-culture of Chilo partellus (Swinhoe)

Cited by 70 publications

References 1 publication

Oviposition Induced Volatile Emissions from African Smallholder Farmers’ Maize Varieties

Oviposition Induced Volatile Emissions from African Smallholder Farmers’ Maize Varieties

Cotesia flavipes Cameron (Hymenoptera: Braconidae) does not exhibit complementary sex determination (ii) Evidence from laboratory experiments

Host recognition and acceptance behaviour in Cotesia sesamiae and C. flavipes (Hymenoptera: Braconidae), parasitoids of gramineous stemborers in Africa

Contact Info

Product

Resources

About