Improvement of tannase production under submerged fermentation by Aspergillus niger FBT1 isolated from a mangrove forest

Abstract

Aspergillus niger FBT1, a local extracellular strain for tannase production, was isolated from soil collected from Matang Mangrove Forest Reserve in Perak, Malaysia. This fungus strain was cultivated in an Erlenmeyer flask under a submerged fermentation system. Medium compositions play a very important role in enhancing enzyme production during fermentation. The production of tannase by A. niger FBT1 increased significantly (95%) when the medium compositions and various process parameters were optimized. Incubation for 72 hours (30EC, pH 7) in medium complemented with sodium nitrate was found optimal. Additional supplementation with tannic acid (2% w/v) as the sole carbon source strongly increased the yield of the enzyme.

Screening, Selection and Optimization of the Culture Conditions for Tannase Production by Endophytic Fungi Isolated from Caatinga

“…4). In general, Aspergillus species are described as tannase producers when maintained at 30 °C, such as A. niger [32], A. aculeatus DBF9 [33], A. tamarii [19] and A. niger FBT1 [28]. However, similar to our results, the temperature of 37 °C was described as optimal for the production of tannase by A. awamori [34] and by A. niger ITCC 6514.07 [35].…”

“…Similar results were observed for the tannase produced by Aspergillus SH6, with increased enzymatic levels in the presence of peptone [43]. A. niger ITCC 6514.07 [35] and A. niger FBT1 [28] showed higher tannase production in the presence of NaNO 3 .…”

“…According to Banerjee et al (2001) [26], the optimal period of fermentation for tannase production can range from 24 h to 150 h. Most studies on the production of tannase by Aspergillus report maximal synthesis of the enzyme after 72 h under SmF, as observed for A. foetidus [27] and A. niger FBT1 [28]. There are also reports of maximum enzyme production after 48 h of cultivation by A. niger [13] and A. tamarii [19].…”

Bioprospecção de tanases produzidas por fungos filamentosos da caatinga.

“…Este ácido pode estar também na composição do meio de produção da enzima como sua única fonte de carbono, e mesmo na presença de outro componente nutricional, a concentração do ácido tânico é indispensável para a produção da tanase. Alguns estudos realizados mostram que a presença de íons de ferro, de zinco e de cobre, também, é considerada essencial para a produção da enzima (PINTO et al, 2005, HAMDY;FAWZY, 2012;GEORGE;ONG, 2013).…”

“…E mesmo na presença de outro componente nutricional, a concentração do ácido tânico é indispensável para a produção da tanase. Alguns estudos realizados mostram que a presença de íons de ferro, de zinco e de cobre, também, é considerada essencial para a produção da enzima (PINTO et al, 2005, HAMDY;FAWZY, 2012;GEORGE;ONG, 2013). Estáveis em uma faixa de pH de 3,5 a 8,0, pH ótimo de 5,5 a 6,0, estabilidade a temperaturas de 30 a 60ºC e temperatura ótima em torno de 30 a 40ºC (AGUILAR; GUTIÉRREZ-SÁNCHEZ, 2001).…”