Improvement of stress tolerance in plants by genetic manipulation of mitogen-activated protein kinases

Šamajová, Olga; Plíhal, Ondřej; Alyousif, Mohamed S; Hirt, Heribert; Šamaj, Jozef

doi:10.1016/j.biotechadv.2011.12.002

Cited by 117 publications

(73 citation statements)

References 143 publications

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“…SUB1A acts as a nodal point of crosstalk in multiple signaling pathways, including ethylene, ROS, brassinosteroids, and GA responses Xu et al, 2006;Fukao and Bailey-Serres, 2008;Jung et al, 2010;Schmitz et al, 2013). The MAPK cascade is also outlined as a stress-activated signal transduction network, conventionally triggered by various cues of ethylene and ROS (Hirt, 2000;Hirt, 2006, 2009a;Pitzschke et al, 2009;Samajová et al, 2013). Furthermore, variations in putative MAPK sites (Ser-186) distinguish the proteins produced by the tolerant (SUB1A1) and intolerant (SUB1A2) alleles of SUB1A .…”

Section: Discussionmentioning

confidence: 99%

A Positive Feedback Loop Governed by SUB1A1 Interaction with MITOGEN-ACTIVATED PROTEIN KINASE3 Imparts Submergence Tolerance in Rice

Singh

Sinha

2016

Plant Cell

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ORCID IDs: 0000-0003-3694-6378 (P.S.); 0000-0003-0820-9936 (A.K.S.)Mitogen-activated protein kinase (MAPK) signal transduction networks have been extensively explored in plants; however, the connection between MAPK signaling cascades and submergence tolerance is currently unknown. The ethylene response factor-like protein SUB1A orchestrates a plethora of responses during submergence stress tolerance in rice (Oryza sativa). In this study, we report that MPK3 is activated by submergence in a SUB1A-dependent manner. MPK3 physically interacts with and phosphorylates SUB1A in a tolerant-allele-specific manner. Furthermore, the tolerant allele SUB1A1 binds to the MPK3 promoter and regulates its expression in a positive regulatory loop during submergence stress signaling. We present molecular and physiological evidence for the key role of the MPK3-SUB1A1 module in acclimation of rice seedlings to the adverse effects of submergence. Overall, the results provide a mechanistic understanding of submergence tolerance in rice.

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Section: Discussionmentioning

confidence: 99%

A Positive Feedback Loop Governed by SUB1A1 Interaction with MITOGEN-ACTIVATED PROTEIN KINASE3 Imparts Submergence Tolerance in Rice

Singh

Sinha

2016

Plant Cell

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“…Regardless, to accommodate the rapidity of the response, there must be preexisting GSR regulators in the cell. A likely GSR regulatory candidate, known to be involved in multiple rapid stress signaling networks across kingdoms, is MITOGEN-ACTIVATED PROTEIN KINASE (MAPK) signaling cascades, which consist of three levels of activating phosphorylation (MAPKKK, MAPKK, and MAPK; Ichimura et al, 1998;Zhang and Klessig, 2001;Nakagami et al, 2005;Andreasson and Ellis, 2010;Rodriguez et al, 2010;Šamajová et al, 2013). Within MAPK signaling cascades, of particular note is MEKK1, a MAPKKK implicated in myriad stress responses through multiple downstream signaling partners (Asai et al, 2002;Nakagami et al, 2005;Hadiarto et al, 2006).…”

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confidence: 99%

Distinct Roles for Mitogen-Activated Protein Kinase Signaling and CALMODULIN-BINDING TRANSCRIPTIONAL ACTIVATOR3 in Regulating the Peak Time and Amplitude of the Plant General Stress Response

et al. 2014

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To survive environmental challenges, plants have evolved tightly regulated response networks, including a rapid and transient general stress response (GSR), followed by well-studied stress-specific responses. The mechanisms underpinning the GSR have remained elusive, but a functional cis-element, the rapid stress response element (RSRE), is known to confer transcription of GSR genes rapidly (5 min) and transiently (peaking 90-120 min after stress) in vivo. To investigate signal transduction events in the GSR, we used a 4xRSRE:LUCIFERASE reporter in Arabidopsis (Arabidopsis thaliana), employing complementary approaches of forward and chemical genetic screens, and identified components regulating peak time versus amplitude of RSRE activity. Specifically, we identified a mutant in CALMODULIN-BINDING TRANSCRIPTIONAL ACTIVATOR3 (CAMTA3) with reduced RSRE activation, verifying this transcription factor's role in activation of the RSRE-mediated GSR. Furthermore, we isolated a mutant in MITOGEN-ACTIVATED PROTEIN KINASE (MAPK) KINASE KINASE1 (mekk1-5), which displays increased basal and an approximately 60-min earlier peak of wound-induced RSRE activation. The double mekk1/camta3 mutant positioned CAMTA3 downstream of MEKK1 and verified their distinct roles in GSR regulation. mekk1-5 displays programmed cell death and overaccumulates reactive oxygen species and salicylic acid, hallmarks of the hypersensitive response, suggesting that the hypersensitive response may play a role in the RSRE phenotype in this mutant. In addition, chemical inhibition studies suggest that the MAPK network is required for the rapid peak of the RSRE response, distinguishing the impact of chronic (mekk1-5) from transient (chemical inhibition) loss of MAPK signaling. Collectively, these results reveal underlying regulatory components of the plant GSR and further define their distinct roles in the regulation of this key biological process.

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“…Based on in silico study of MPK4 and MEKK1/MKK1/MKK2 complex interaction using the Pathway Studio software, it was reported that all four related genes were involved in the regulation of defense response. In a study of the genetic manipulation of MAP kinase cascade in plants proposed that the MPK4, MEKK1, and MKK1/MKK2 complex negatively regulated defense responses [38]. Based on this result, we predict that papaya MPK4 and MEKK1 also regulate defense response by suppressing it once it is activated.…”

Section: Gene-gene Interaction In Defense Responsementioning

confidence: 83%

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2017

Bioinformation

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Abstract:Mitogen-activated protein kinase 4 (MPK4) interacts with the (Mitogen-activated protein kinase kinase kinase 1) MEKK1/ Mitogenactivated protein kinase kinase 1 (MKK1)/ Mitogen-activated protein kinase kinase 2 (MKK2) complex to affect its function in plant development or against pathogen attacks. The KEGG (Kyoto Encyclopedia of Genes and Genomes) network analysis of Arabidopsis thaliana revealed close interactions between those four genes in the same plant-pathogen interaction pathway, which warrants further study of these genes due to their evolutionary conservation in different plant species. Through targeting the signature sequence in MPK4 of papaya using orthologs from Arabidopsis, the predicted sequence of MPK4 was studied using a comparative in silico approach between different plant species and the MAP cascade complex of MEKK1/MKK1/MKK2. This paper reported that MPK4 was highly conserved in papaya with 93% identical across more than 500 bases compared in each species predicted. Slight variations found in the MEKK1/MKK1/MKK2 complex nevertheless still illustrated sequence similarities between most of the species. Localization of each gene in the cascade network was also predicted, potentiating future functional verification of these genes interactions using knock out or/and gene silencing tactics.

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Improvement of stress tolerance in plants by genetic manipulation of mitogen-activated protein kinases

Cited by 117 publications

References 143 publications

A Positive Feedback Loop Governed by SUB1A1 Interaction with MITOGEN-ACTIVATED PROTEIN KINASE3 Imparts Submergence Tolerance in Rice

A Positive Feedback Loop Governed by SUB1A1 Interaction with MITOGEN-ACTIVATED PROTEIN KINASE3 Imparts Submergence Tolerance in Rice

Distinct Roles for Mitogen-Activated Protein Kinase Signaling and CALMODULIN-BINDING TRANSCRIPTIONAL ACTIVATOR3 in Regulating the Peak Time and Amplitude of the Plant General Stress Response

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