Improvement of somatic embryogenesis and plant recovery in cassava

Mathews, Helena; Schöpke, C.; Carcamo, R.; Chavarriaga, Paul; Fauquet, Claude; Beachy, Roger N.

doi:10.1007/bf00237429

Cited by 74 publications

(36 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…31 Desiccation also has been reported to promote somatic embryo differentiation and development to other crops like as soybean, 36,37 wheat, 38 spruce 39 and cassava. 40 In recent years tissue culture techniques are being used as a useful tool to elucidate the mechanism involved in salt tolerance. 41,42 Several metals are essential nutrients for plants, yet they become toxic at high levels and deleteriously affect crop yield and quality.…”

Section: Introductionmentioning

confidence: 99%

Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

Siddique

Ara

Islam

et al. 2014

Plant Signaling & Behavior

View full text Add to dashboard Cite

D number of viable callus; ICn D number of inoculated callus.Enhancement of callus induction and its regeneration efficiency through in vitro techniques has been optimized for 2 abiotic stresses (salt and air desiccation) using 3 rice genotypes viz. BR10, BRRI dhan32 and BRRI dhan47. The highest frequency of callus induction was obtained for BRRI dhan32 (64.44%) in MS medium supplemented with 2, 4-D (2.5 mgL ¡1) and Kin (1.0 mgL ¡1). Different concentrations of NaCl (2.9, 5.9, 8.8 and 11.7 gL ¡1 ) were used and its effect was recorded on the basis of viability of calli (VC), relative growth rate (RGR), tolerance index (TI) and relative water content (RWC). It was observed that in all cases BRRI dhan47 showed highest performance on tolerance to VC (45.33%), RGR (1.03%), TI (0.20%) and RWC (10.23%) with 11.7 gL ¡1 NaCl. Plant regeneration capability was recorded after partial air desiccation pretreatment to calli for 15, 30, 45 and 60 h. In this case BRRI dhan32 gave maximum number of regeneration (76.19%) when 4 weeks old calli were desiccated for 45 h. It was observed that air desiccation was 2-3 folds more effective for enhancing green plantlet regeneration compared to controls. Furthermore, desiccated calli also showed the better capability to survive in NaCl induced abiotic stress; and gave 1.9 fold (88.80%) increased regeneration in 11.7 gL ¡1 salt level for BRRI dhan47. Analysis of variance (ANOVA) showed that the genotypes, air desiccation and NaCl had significant effect on plant regeneration at P < 0.01.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

Siddique

Ara

Islam

et al. 2014

Plant Signaling & Behavior

View full text Add to dashboard Cite

show abstract

“…It has been shown that the efficiency of somatic embryo maturation and plant conversion can be improved via the use of abscisic acid (ABA) which is known to synchronise embryo maturation (Groll et al 2002;Quainoo and Dwomon 2012), confer desiccation tolerance (Torres et al 2001) and enhance plant conversion (Quainoo and Dwomon 2012). Also, desiccation of somatic embryos prior to culture on standard cassava medium has been used to enhance plant conversion (Matthews et al 1993). The protocol described below aims to optimise cassava somatic embryo induction at both the primary and the cyclic stage using the synthetic auxins 2,4-D and picloram.…”

Section: Somatic Embryo Conversion Into Plantsmentioning

confidence: 99%

Optimisation of Somatic Embryogenesis in Cassava

Danso

Elegba

2016

Biotechnologies for Plant Mutation Breeding

View full text Add to dashboard Cite

Somatic embryogenesis, an efficient regeneration system that is being used successfully in genetic transformation, can be coupled with mutation induction to breed for cassava varieties with desired traits. The embryogenesis system requires the initiation of totipotent cells which can be used as targets for mutagenic treatments. However, initiation of these totipotent cells is low, highly genotypic dependent and has low plant conversion rate. Thus, for its successful application in mutation induction, the system needs to be optimised to overcome these drawbacks. To optimise the system, totipotent cells are initiated on embryo initiation medium (EIM) which consisted of Murashige and Skoog (Physiol Plant 15:473-497, 1962) basal salts supplemented with picloram and 2,4-dichlorophenoxy acetic acid (2,4-D) to produce callus. After 21 days, calli produced are cultured for embryo maturation on maturation medium (EMM) or can also be treated with mutagenic agents for mutant induction. The resulting primary embryos are recycled three consecutive times, each by fragmentation of the cotyledons followed by culture on embryo initiation medium to produce more matured embryos. Somatic embryos produced are then successfully converted into plants by abscisic acid pretreatment in embryo maturation medium or by air desiccation under the laminar flow hood. The methodology described offers optimised, reproducible procedures of somatic embryogenesis for its incorporation into mutation breeding programmes in cassava.

show abstract

“…Partial desiccation has been reported to accelerate plant organogenesis or embryogenesis and results in high regeneration ability significantly in grape (Gray, 1989), wheat (Cheng et al, 2003), Brassica napus (Kott & Beversdorf, 1990), rice (Rance et al, 1994;Saharan et al, 2004;Tsukahara & Hirosawa, 1992) and cassava (Mathews et al, 1993). The possible mechanism about its promotion on plant regeneration capacity may be that partial desiccation terminates the developmental mode and "switches" the embryo into a germination mode (Attree et al, 1991).…”

Section: Partial Desiccationmentioning

confidence: 99%