Improvement of simultaneous determination of neutral monosaccharides and uronic acids by gas chromatography

Wang, Xin; Zhang, Lihui; Wu, Jingli; Xu, Wenbin; Wang, Xiaoqin; Lü, Xin

doi:10.1016/j.foodchem.2016.10.008

Cited by 89 publications

(40 citation statements)

References 37 publications

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“…[21] Monosaccharide composition analysis: The monosaccharide composition analysis of GZMP-1, GZMP-2, GZMP-3, and GZMP-4 was detected by GC analysis as described previously. [22,23] GC (Shimadzu 2014 C; Shimadzu Corp., Kyoto, Japan) with a high-performance capillary column (DB-17; 30 mL × 0.25 mm ID, 0.25-μm film thickness; Agilent Technologies, Santa Clara, CA, USA) was used to determine the neutral monosaccharides' derivatized products.…”

Section: Methodsmentioning

confidence: 99%

Isolation, purification, and antioxidant activities of polysaccharides from Ziziphus Jujuba cv. Muzao

Liu

Ullah

et al. 2018

International Journal of Food Properties

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In the present study, crude polysaccharides from Ziziphus Jujuba cv. Muzao were isolated and purified using DEAE cellulose-52 and Sephadex G-100 size-exclusion chromatography; four fractions were collected, namely GZMP-1, GZMP-2, GZMP-3, and GZMP-4. The molecular weights of these four fractions were measured to be 111.2, 95.1, 84.2, and 571.4 kDa, respectively, using high-performance gel permeation chromatography. Gas chromatography analysis of the monosaccharide composition confirmed that GZMP-1 was composed of rhamnose, arabinose, glucose, and galactose. Rhamnose, arabinose, and galactose were the main components present in GZMP-2 and GZMP-3, whereas GZMP-4 was composed of only rhamnose and arabinose. Scanning electron microscopy showed relatively smooth surfaces for GZMP-1 and GZMP-4, whereas GZMP-2 and GZMP-3 had more folds on their surfaces. Fourier transform infrared spectroscopy analyses indicated that GZMP-1 and ZMP mainly had α-type glycosidic linkages. The in vitro antioxidant activities of the polysaccharides revealed that jujube polysaccharides exhibit remarkable antioxidant activity, and can scavenge DPPH radical and OH radical in a concentration-dependent manner. The results of this work suggest that polysaccharides from Z. Jujuba cv. Muzao have potential to be used as functional food and in the development of natural antioxidant drug carriers. ARTICLE HISTORY

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Section: Methodsmentioning

confidence: 99%

Isolation, purification, and antioxidant activities of polysaccharides from Ziziphus Jujuba cv. Muzao

Liu

Ullah

et al. 2018

International Journal of Food Properties

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“…Hydrolysis of EPSs sample was performed with 2 M trifluoroacetic acid (120 °C, 2 h) and the monosaccharides were reduced with NaBH 4 , neutralised, acetylated as described by Wang et al . () and then analysed by GC‐MS as described by Sun et al . ().…”

Section: Methodsmentioning

confidence: 99%

The effect of litchi juice on exopolysaccharide production in milk fermented by Lactobacillus casei

et al. 2018

Int J of Food Sci Tech

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Summary In this paper, Lactobacillus casei growth, sugar utilisation, exopolysaccharides (EPSs) formation and EPSs characterisation during fermentation of milk with or without litchi juice were evaluated. During L. casei fermentation of litchi‐milk samples, the EPSs content increased with the increase of litchi juice levels (0–25%, v/v), and the both relationship appeared in S‐type curve. The viscosity of fermented milk also significantly increased with the formation of EPSs, and which positively correlated with the EPSs content (R2 = 0.9782). The EPSs from the fermented litchi‐milk samples was isolated for further structural characterisation. Monosaccharide and molecular weight analysis of purified EPSs sample proved that it contained no carbohydrate other than glucose, and its molecular weight was calibrated as 3.03 × 106 Da. FT‐IR and NMR (1H and 13C) spectroscopy analysis confirmed the synthesised EPSs sample was α‐glucans (dextrans) with mainly α‐(1→6) linkages. To the best of our knowledge, this is the first study to report that litchi juice can enhance the EPSs (dextrans, mainly α‐(1→6) linkages) production in fermented milk.

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“…The coefficients of variance were lower than 5% for most compounds and 5.2%-7.2% for the others including GalNAc, GlcNAc, and fructose based on four replicates. Compared to other recently published quantitation methods for monosaccharides including GC-FID, CE-UV, HPAEC-PAD, and LC-MS using an ion trap instrument (Table S1 ) , 37–40 the current method employing UHPLC-QqQ with dMRM has proven to be the fastest and most sensitive, while covering a wide range of monosaccharides. Owing to the efficient separation, minimal interference, and targeted detection, the LODs for most PMP-derivatized monosaccharides were improved by > 1000-fold to 0.056-5.6 femtomoles compared to ≥ 9 or 0.7 picomoles obtained by UV or ion trap MS, respectively.…”

Section: Resultsmentioning

confidence: 95%

Revisiting monosaccharide analysis – quantitation of a comprehensive set of monosaccharides using dynamic multiple reaction monitoring

Amicucci

Cheng

et al. 2018

Analyst

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A rapid method for the quantitation of sixteen neutral and acidic monosaccharides, from both animal and plant sources was developed using ultra-high performance liquid chromatography triple quadrupole mass spectrometry (UHPLC/QqQ-MS) in dynamic multiple reaction monitoring (dMRM) mode. Monosaccharides including three pentoses (ribose, xylose, arabinose), two deoxyhexoses (rhamnose, fucose), five hexoses (fructose, mannose, allose, glucose, galactose), two hexuronic acids (glucuronic acid, galacturonic acid), and two N-acetyl-hexosamines (GlcNAc, GalNAc), were derivatized with 1-phenyl-3-methyl-5-pyrazolone (PMP), while underivatized sialic acids, N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), were simultaneously analyzed with a 10-minute run. With the optimized UHPLC conditions, baseline separations of the isomers were achieved. The sensitivity and calibration ranges of this method were determined. The limits of detection were between femtomoles and attomoles with linear ranges spanning four to six orders of magnitude and CVs ≤ 7.2%. Spiking experiments performed on a pooled fecal sample demonstrated the high accuracy of this method even when applied to samples with complicated matrices. The validated method was applied to fecal samples from an infant transitioning from breast milk to weaning foods. Major milk monosaccharides including galactose, fucose, glucose, GlcNAc, and Neu5Ac were found to be the most abundant components in the feces of milk-fed infants. PMP-derivatives of other monosaccharides including apiose, lyxose, altrose, talose, gulose, glucosamine, galactosamine, mannosamine, and N-acetylmannosamine (ManNAc) were also tested and could be added to the quantitation method depending on the need. The speed and sensitivity of the method makes it readily adaptable to rapid throughput analysis of monosaccharides in biological samples.

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Improvement of simultaneous determination of neutral monosaccharides and uronic acids by gas chromatography

Cited by 89 publications

References 37 publications

Isolation, purification, and antioxidant activities of polysaccharides from Ziziphus Jujuba cv. Muzao

Isolation, purification, and antioxidant activities of polysaccharides from Ziziphus Jujuba cv. Muzao

The effect of litchi juice on exopolysaccharide production in milk fermented by Lactobacillus casei

Revisiting monosaccharide analysis – quantitation of a comprehensive set of monosaccharides using dynamic multiple reaction monitoring

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