Improvement of pregnancy outcome by extending embryo culture in IVF-ET during clinical application

Zhao, Xiao Li; Ma, Baoshan; Mo, Shao-Kang; Ma, Lu; Chang, Fei; Zhang, Liyuan; Xu, Fang

doi:10.1007/s10815-017-1065-5

Cited by 9 publications

(8 citation statements)

References 29 publications

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“…[ 4 ] Thus, day 3 post-fertilization is a critical period for human embryos to initiate EGA. Consistent with previous studies, [ 3 , 4 ] most available embryos (80.47%) in our study developed to the 4 to 8-cell stage on the morning of day 3. The EC of 7 to 8 h provided a short time for viable embryos to trigger EGA, and some embryos with SDP (57.63%) continued developing.…”

supporting

confidence: 93%

“…Thus, implantation rates remain at 20% to 30% in IVF-ET. [ 3 ] During IVF-ET treatment, we found that more than half of day 3 embryos continued developing during an extended culture (EC) of 7 to 8 h from 08:00–09:00 to 16:00, and live birth rates increased when these embryos were transferred. Then, this strategy was used to improve live birth rates during IVF-ET.…”

mentioning

confidence: 99%

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Extended culture of day 3 embryos improves live birth rate in in vitro fertilization-embryo transfer

Zhao

Wang

et al. 2020

Chinese Medical Journal

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supporting

confidence: 93%

mentioning

confidence: 99%

Extended culture of day 3 embryos improves live birth rate in in vitro fertilization-embryo transfer

Zhao

Wang

et al. 2020

Chinese Medical Journal

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“…In a clinical context, normal embryos should develop at the 8-cell stage 3 days after IVF 31 ; however, there were embryos that were fertilized, as evidenced by the formation of pronuclei, that remained arrested at the 1-cell stage 32 . Zygotic arrest of some embryos was due to gene mutations, such as TUBB8 mutations that affect cell division 33 .…”

Section: Resultsmentioning

confidence: 99%

Dynamics and clinical relevance of maternal mRNA clearance during the oocyte-to-embryo transition in humans

et al. 2020

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Maternal mRNA clearance is an essential process that occurs during maternal-to-zygotic transition (MZT). However, the dynamics, functional importance, and pathological relevance of maternal mRNA decay in human preimplantation embryos have not yet been analyzed. Here we report the zygotic genome activation (ZGA)-dependent and -independent maternal mRNA clearance processes during human MZT and demonstrate that subgroups of human maternal transcripts are sequentially removed by maternal (M)- and zygotic (Z)-decay pathways before and after ZGA. Key factors regulating M-decay and Z-decay pathways in mouse have similar expression pattern during human MZT, suggesting that YAP1-TEAD4 transcription activators, TUT4/7-mediated mRNA 3ʹ-oligouridylation, and BTG4/CCR4-NOT-induced mRNA deadenylation may also be involved in the regulation of human maternal mRNA stability. Decreased expression of these factors and abnormal accumulation of maternal transcripts are observed in the development-arrested embryos of patients who seek assisted reproduction. Defects of M-decay and Z-decay are detected with high incidence in embryos that are arrested at the zygote and 8-cell stages, respectively. In addition, M-decay is not found to be affected by maternal TUBB8 mutations, although these mutations cause meiotic cell division defects and zygotic arrest, which indicates that mRNA decay is regulated independent of meiotic spindle assembly. Considering the correlations between maternal mRNA decay defects and early developmental arrest of in vitro fertilized human embryos, M-decay and Z-decay pathway activities may contribute to the developmental potential of human preimplantation embryos.

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“…In this study, we used light (LM) and transmission electron microscopy (TEM) to describe the morphological changes occurring in pre-implantation mouse embryos (at the 2-cell, 4-cell, morula and blastocyst stage), following in vitro culture under physiologic (5%) and atmospheric (20%) concentrations of O 2, using in vivo embryos flushed out of the uterus as controls. Furthermore, we focused our analysis on the blastocyst stage, since embryo transfer at this stage is common, with increased occurrence of healthy singleton live birth [20,21]. These data will help us to understand how variations of O 2 concentrations can affect, to what extent and at which stage, the ultrastructure of murine embryos cultured in vitro.…”

Section: Introductionmentioning

confidence: 99%

Pre-Implantation Mouse Embryos Cultured In Vitro under Different Oxygen Concentrations Show Altered Ultrastructures

Belli

Rinaudo

Palmerini

et al. 2020

IJERPH

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Assisted Reproductive Technologies routinely utilize different culture media and oxygen (O2) concentrations to culture human embryos. Overall, embryos cultured under physiological O2 tension (5%) have improved development compared to embryos cultured under atmospheric O2 conditions (20%). The mechanisms responsible for this remain unclear. This study aimed to evaluate the effect of physiologic (5%) or atmospheric O2 (20%) tension on the microscopic ultrastructure of pre-implantation mouse embryos using Transmission Electron Microscopy (TEM). Embryos flushed out of the uterus after natural mating were used as the control. For use as the control, 2-cells, 4-cells, morulae, and blastocysts were flushed out of the uterus after natural fertilization. In vitro fertilization (IVF) was performed using potassium simplex optimized medium (KSOM) under different O2 tensions (5% and 20%) until the blastocyst stage. After collection, embryos were subjected to the standard preparative for light microscopy (LM) and TEM. We found that culture in vitro under 5% and 20% O2 results in an increase of vacuolated shaped mitochondria, cytoplasmic vacuolization and presence of multi-vesicular bodies at every embryonic stage. In addition, blastocysts generated by IVF under 5% and 20% O2 showed a lower content of heterochromatin, an interruption of the trophectodermal and inner cell mass cell membranes, an increased density of residual bodies, and high levels of glycogen granules in the cytoplasm. In conclusion, this study suggests that in vitro culture, particularly under atmospheric O2 tension, causes stage-specific changes in preimplantation embryo ultrastructure. In addition, atmospheric (20%) O2 is associated with increased alterations in embryonic ultrastructure; these changes may explain the reduced embryonic development of embryos cultured with 20% O2.

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Improvement of pregnancy outcome by extending embryo culture in IVF-ET during clinical application

Abstract: The extended culture of day 3 embryos for 7-8 h not only reduced the risk of IVF-ET treatment compared to blastocyst culture through another 2-3 days, but also improved the clinical outcomes and the efficiency of every transferred cycle and every transferred embryo.

Cited by 9 publications

References 29 publications

Extended culture of day 3 embryos improves live birth rate in in vitro fertilization-embryo transfer

Extended culture of day 3 embryos improves live birth rate in in vitro fertilization-embryo transfer

Dynamics and clinical relevance of maternal mRNA clearance during the oocyte-to-embryo transition in humans

Pre-Implantation Mouse Embryos Cultured In Vitro under Different Oxygen Concentrations Show Altered Ultrastructures

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