Improvement of Polyethylene Glycol, Sorbitol, Mannitol, and Sucrose-Induced Osmotic Stress Tolerance through Modulation of the Polyamines, Proteins, and Superoxide Dismutase Activity in Potato

Abstract

The present study was planned to investigate the changes in morphological and biochemical parameters of in vitro-grown potato (cultivar Cardinal and Desiree) plants under osmotic stress conditions induced by various concentrations of sorbitol, mannitol (0, 0.025, 0.05, 0.10, or 0.15 M), sucrose (0, 2, 3, 4, 6, and 8%), and polyethylene glycol (PEG: MW-4000: 0, 5, 10, 15, and 20%). Nodal segments (ca. 1.0 cm) from healthy in vitro-grown potato plantlets were inoculated on Murashige and Skoog’s medium consisting of various levels of above mentioned drought stress-inducing agents. Data was recorded on 60th day of incubation exhibited a severe reduction in most of the growth parameters at 0.10 and 0.15 M of sorbitol and mannitol, respectively, and at 5–10% PEG. Similar results were observed when the sucrose level varied from 3% except for the number of roots and plant dry weight, which exhibited an increase in increasing the sucrose level. Data collected for total soluble protein content and activity of an antioxidant enzyme (superoxide dismutase) unveiled an overall increasing trend in osmotically stressed potato plants suggesting their major action in detoxification of active oxygen species produced under osmotic stress. Polyamines (putrescine, spermidine, and spermine) increased significantly in both the cultivars of potato by using osmotic stress-inducing agent in the present investigation indicating their positive role in stress alleviation. Overall results indicated that potato cultivar Desiree was more stress-tolerant than the cultivar Cardinal.

Ameliorative Role of Ascorbic acid to Drought Stress in Hordeum Vulgare L. through Modulation of the polyamines, osmolytes and Antioxidant Defense System

“…Polyamines have protective role on plants under various abiotic stress by regulating and increase the uptake of various inorganic ions directly which in turn enhance stress tolerance in plants (Sajid and Aftab, 2022). During this investigation all the polyamines (Put, spm, spd) increase significantly in the studied verities of Barley.…”

“…Polyamines (PAs) are essential compounds that participate in various metabolic processes in plants. They are produced in both stressed and normal conditions (Sajid and Aftab, 2022). PAs play a regulatory role in plants under osmotic stress conditions by increasing the concentration of Ca, K, Fe, Mn, Zn, and NO-3 ions in Lettuce leaves, as well as reducing stomatal aperture .…”

Screening Smoke Tree (Cotinus coggygria Scop.) on Osmotic Stress using Polyethylene Glycol 6000 in vitro

“…Currently, systems for selection of drought-resistant forms are used for major crops such as wheat (Faisal et al 2019), rice (Purbajanti et al, 2019;Veronica et al, 2022), barley (Hellal et al, 2018), chickpea (Hussain et al, 2022), potatoes (Hanasz et al, 2022Sajid and Aftab, 2022), alfalfa (Elmaghrabi et al, 2018) and sorg hum (Badigannavar et al, 2018). At the same time, the osmotic influence on tree species in vitro is mentioned less often (Ahmad et al, 2020;Gul et al, 2021;Pasaribu et al, 2021;Vuksanovic et al, 2022).…”