Improvement of Plant Regeneration from Immature Embryos of Wheat Infected by Agrobacterium tumefaciens

Tao, Li-Li; Yin, Gui-xiang; Du, Liangcheng; Shi, Zheng‐yuan; She, Maoyun; Xu, Huijun; Ye, Xingguo

doi:10.1016/s1671-2927(11)60010-2

Cited by 28 publications

(14 citation statements)

References 32 publications

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“…lunxuan987) via Agrobacterium-mediated transformation as described by Tao et al (2011). The TaTOC1 knockdown (referred to as TaTOC1-Ri) transgene was detected by a positive PCR product (478 bp) amplified with specific to pTCK303-D, located in the rice intron region and TaTRNi-R, in the TaTOC1 coding region.…”

Section: Constructs and Plant Transformationmentioning

confidence: 99%

The tae-miR408-Mediated Control of TaTOC1 Genes Transcription Is Required for the Regulation of Heading Time in Wheat

Zhao

Hong

et al. 2016

Plant Physiol.

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ORCID IDs: 0000-0002-1560-7935 (J.Y.W.); 0000-0002-3129-5206 (X.S.Z.).Timing of flowering is not only an interesting topic in developmental biology, but it also plays a significant role in agriculture for its effects on the maturation time of seed. The hexaploid wheat (Triticum aestivum) is one of the most important crop species whose flowering time, i.e. heading time, greatly influences yield. However, it remains unclear whether and how microRNAs regulate heading time in it. In our current study, we identified the tae-miR408 in wheat and its targets in vivo, including Triticum aestivum TIMING OF CAB EXPRESSION-A1 (TaTOC-A1), TaTOC-B1, and TaTOC-D1. The tae-miR408 levels were reciprocal to those of TaTOC1s under long-day and short-day conditions. Wheat plants with a knockdown of TaTOC1s via RNA interference and overexpression of tae-miR408 showed early-heading phenotype. Furthermore, TaTOC1s expression was down-regulated by the tae-miR408 in the hexaploid wheat. In addition, other important agronomic traits in wheat, such as plant height and flag leaf angle, were regulated by both tae-miR408 and TaTOC1s. Thus, our results suggested that the tae-miR408 functions in the wheat heading time by mediating TaTOC1s expression, and the study provides important new information on the mechanism underlying heading time regulation in wheat.

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Section: Constructs and Plant Transformationmentioning

confidence: 99%

The tae-miR408-Mediated Control of TaTOC1 Genes Transcription Is Required for the Regulation of Heading Time in Wheat

Zhao

Hong

et al. 2016

Plant Physiol.

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“…Hence, to facilitate a further development of transformed wheat cells we included ascorbic acid in all further Agrobacterium-mediated transformation experiments in wheat. Oppositely, Tao et al (2011) found that the wheat transformed-calli growth status was not improved by adding anti-oxidants ascorbic acid, cysteine and silver nitrate. Compared to the present study, they used the same concentration of ascorbic acid, but wheat genotypes, transformation vector, concentration of acetosyringone, which increases susceptibility to Agrobacterium, and addition of other organic compounds in suspension liquid medium were different.…”

Section: Discussionmentioning

confidence: 98%

“…The first fertile transgenic wheat plants have been obtained by Vasil et al (1992) using the microprojectile bombardment transformation method. Agrobacterium-mediated transformation has been demonstrated as a better alternative to biolistic gun for low copy insertions of T-DNA with minimal rearrangements and degree of gene-silencing phenomenon (Cheng et al 2004;Jones et al 2005;Shrawat & Lörz 2006;Tao et al 2011).…”

Section: Introductionmentioning

confidence: 99%

The procedure providing enhanced Agrobacterium-mediated transformation of wheat

et al. 2014

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The examinations of conditions for establishing a variety independent Agrobacterium-mediated transformation procedure for wheat are preferable since many of cultivars and breeding lines remain recalcitrant for biotechnological manipulation, mainly due to low efficiency of plant regeneration in vitro, which is highly genotype specific. This paper describes and discusses an improved protocol for enhanced and low-genotype dependent Agrobacterium-mediated transformation using a super-binary vector LBA4404/pTOK233 carrying reporter gus-intron gene and hygromycin (hpt) and kanamicyn (nptII) selectable marker genes. The protocol was optimized on highly responsive common wheat cv. Vesna. Transient expression monitored by the gus-intron on explants after 3, 6 and 25 days of co-cultivation, followed by GUS expression and hygromycin resistance in whole plants indicated the protocol including a co-cultivation of freshly isolated immature embryos in the presence of ascorbic acid, and acetosyringone added only in the bacteria-containing infection medium combined with a delayed and stepwise increasing hygromycin B selection procedure significantly enhanced the transformation efficiency in cv. Vesna that exceed 7% of treated explants from previously 0.41%. Explant pre-cultivation did not additionally improve transformation efficiency. The optimized protocol was successful in evoking satisfactory transformation efficiencies from 3.6% to 10.8% in 5 less-responsive wheat genotypes. All 57 T0 hygromycin-resistant and GUS-positive lines were phenotypically normal and fertile. Therefore, the conditions employed in this study may serve as a base to facilitate the transformation in other, particularly recalcitrant wheat cultivars.

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“…Various explants have been tested in wheat tissue culture, including immature embryos, mature embryos, anthers, and young panicle (Barro et al 1999). Research (Harwood 2012;Tao et al 2011) has shown that immature embryos have the highest callus induction efficiency as well as regeneration rate, and are the most frequently used explant in wheat tissue culture. On the other hand, immature embryos are not available at all times, as they can only be collected for several days after anthesis, resulting in mature embryos from mature seeds also being widely used.…”

Section: Introductionmentioning

confidence: 99%

Analysis of biochemical and physiological changes in wheat tissue culture using different germplasms and explant types

Yang

Wang

et al. 2015

Acta Physiol Plant

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To select adequate wheat germplasms for genetic transformation, tissue culture efficiency of 21 different wheat lines (Einkorn, Emmer, Durum wheat, etc.) were compared, along with two different explants, namely, immature embryo and mature embryo. The results showed that the average differentiation rate and regeneration rate of immature embryo calli (46.5 and 20.82 %) were better than those for mature embryo calli (14.03 and 4.37 %). The best genotypes for immature embryo callus culture were 'Ningchun 16' and 'Ei 15', 'Xiaoyan 22', followed by 'Durum 332' and 'Tr 256'. The best genotypes for mature embryo callus culture were 'Ying 4286', 'Yunyin 01', and 'Xiaoyan 22'. To analyze how physiological and biochemical settings influence the totipotency of calli, different physiological and biochemical indices were analyzed. Differences between immature embryo callus and mature embryo callus were significant, as well as differences of most indices among different wheat types. The interaction effects between explant types and genotypes were also significant. Correlation analysis results showed that the total phenol and soluble sugar contents were significantly correlated with callus differentiation and regeneration rates.

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Improvement of Plant Regeneration from Immature Embryos of Wheat Infected by Agrobacterium tumefaciens

Cited by 28 publications

References 32 publications

The tae-miR408-Mediated Control of TaTOC1 Genes Transcription Is Required for the Regulation of Heading Time in Wheat

The tae-miR408-Mediated Control of TaTOC1 Genes Transcription Is Required for the Regulation of Heading Time in Wheat

The procedure providing enhanced Agrobacterium-mediated transformation of wheat

Analysis of biochemical and physiological changes in wheat tissue culture using different germplasms and explant types

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