Improvement of peanut rhizobial inoculant by incorporation of plant growth promoting rhizobacteria (PGPR) as biocontrol against the seed borne fungus, Aspergillus niger

Yuttavanichakul, Watcharin; Lawongsa, Pruksa; Wongkaew, Sopone; Teaumroong, Neung; Boonkerd, Nantakorn; Nomura, Nobuhiko; Tittabutr, Panlada

doi:10.1016/j.biocontrol.2012.06.008

Cited by 68 publications

(25 citation statements)

References 58 publications

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“…Biofilm production was evaluated using the microtiter plate assay (35) with the following modifications: single and combined inoculants (1:1 of each strain) (100 μL) of 2-d-old cultures in R2A broth were transferred into a 96-well polystyrene microtiter plate and incubated at 30°C for 12 h. After loosely associated bacteria were removed, wells were washed with sterilized distilled water, air dried, and then stained with 1% crystal violet solution (150 μL) for 45 min. The wells were washed again with distilled water, destained with 95% ethanol (200 μL), and 100 μL from each well was then transferred to new microtiter plates.…”

Section: Methodsmentioning

confidence: 99%

Bacterial Compatibility in Combined Inoculations Enhances the Growth of Potato Seedlings

Santiago

Yagi

Ijima

et al. 2017

Microbes and environments

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The compatibility of strains is crucial for formulating bioinoculants that promote plant growth. We herein assessed the compatibility of four potential bioinoculants isolated from potato roots and tubers (Sphingomonas sp. T168, Streptomyces sp. R170, Streptomyces sp. R181, and Methylibium sp. R182) that were co-inoculated in order to improve plant growth. We screened these strains using biochemical tests, and the results obtained showed that R170 had the highest potential as a bioinoculant, as indicated by its significant ability to produce plant growth-promoting substances, its higher tolerance against NaCl (2%) and AlCl3 (0.01%), and growth in a wider range of pH values (5.0–10.0) than the other three strains. Therefore, the compatibility of R170 with other strains was tested in combined inoculations, and the results showed that the co-inoculation of R170 with T168 or R182 synergistically increased plant weight over un-inoculated controls, indicating the compatibility of strains based on the increased production of plant growth promoters such as indole-3-acetic acid (IAA) and siderophores as well as co-localization on roots. However, a parallel test using strain R181, which is the same Streptomyces genus as R170, showed incompatibility with T168 and R182, as revealed by weaker plant growth promotion and a lack of co-localization. Collectively, our results suggest that compatibility among bacterial inoculants is important for efficient plant growth promotion, and that R170 has potential as a useful bioinoculant, particularly in combined inoculations that contain compatible bacteria.

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Section: Methodsmentioning

confidence: 99%

Bacterial Compatibility in Combined Inoculations Enhances the Growth of Potato Seedlings

Santiago

Yagi

Ijima

et al. 2017

Microbes and environments

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“…Antibiotic production by selected actinomycetes (4ER99, 4ER116, 9X161, and 9X166) was examined following the method of Yuttavanichakul . The four selected antagonistic isolates were grown for 7 days in ISP‐2 medium.…”

Section: Methodsmentioning

confidence: 99%

Antagonistic activity of endo‐β‐1,3‐glucanase from a novel isolate, Streptomyces sp. 9X166, against black rot in orchids

Sakdapetsiri

Fukuta

Aramsirirujiwet

et al. 2016

J. Basic Microbiol.

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A total of 123 actinomycetes was isolated from 12 varieties of wild orchids and screened for potential antagonistic activity against Phytophthora, which causes black rot disease in orchids. In vitro and in vivo experimental results revealed that Streptomyces sp. strain 9X166 showed the highest antagonistic activity; its β-1,3-glucanase production ability was a key mechanism for growth inhibition of the pathogen. PCR amplification and DNA sequencing of the 16S ribosomal RNA gene allowed the identification of this strain, with high similarity (99.93%) to the novel species Streptomyces similaensis. The glucanase enzyme, purified to homogeneity by anion exchange and gel filtration chromatography, showed a specific activity of 58 U mg(-1) (a 3.9-fold increase) and yield of 6.4%. The molecular weight, as determined by SDS-PAGE and gel filtration, was approximately 99 and 80 kDa, respectively, suggesting that the enzyme was a monomer. The purified enzyme showed the highest substrate specificity to laminarin, indicating that it was β-1,3-glucanase. The hydrolyzed products of cello-oligosaccharides suggested that this enzyme was endo-type β-1,3-glucanase. Streptomyces sp. 9X166 culture filtrate, possessing β-1,3-glucanase activity, could degrade both freeze-dried and living mycelium. This is the first report on a β-1,3-glucanase-producing Streptomyces sp. that could be an effective biocontrol agent for black rot disease in orchids.

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“…All but two isolates had plant growth promoting attributes, but different isolates imparted this function to different parts of the host plant (stem length, root length, leaf number or root number). Similarly, Yuttavanichakul et al ( 2012 ) inoculated roots of peanut with PGPR and inhibited the seed borne pathogen Aspergillus niger . The Bradyrhizobium spp.…”

Section: Anti-fungal Properties Of the Rhizospherementioning

confidence: 98%

Introduction: Soils and Their Promotion of Plant Growth

Dighton

2014

Interactions in Soil: Promoting Plant Growth

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Soil is a complex milieu of physical and biological entities that regulates the availability of nutrients for plant growth. The interactions between the elements of the soil biota and the plants during this process are complex and often rely on feedbacks between the plants and the great diversity of soil organisms that function to regulate processes to support plant growth. The rhizosphere and its mycorrhizal component are important parts of the connection between the plant and soil through its roots. This zone is a 'hot-spot' of microbial activity and trophic interactions with other soil organisms. It is here that growth of the plant root is infl uenced by nutrient availability, induction of root growth stimulating auxins, the interaction with plant pathogens and herbivores. Ecosystem engineers in the form of earthworms and other burrowing fauna are important in altering both the physical structure of soil and rates of decomposition of organic residues. A little researched component of soil is viruses. Their interaction with microbial and faunal communities is introduced here. Many of the soil processes infl uencing plant growth and the diversity of soil organisms is infl uenced by human activities ranging from agricultural and forestry practices through urbanization to the infl uence of pollution from industrial processes. To help us with the understanding of the interactions between biodiversity and function, new molecular tools involving metagenomics and transcriptomics are evolving, and are discussed here as an emerging suite of tools that can be applied to soil ecology. The chapter introduces the detail to come in subsequent chapters of this book.

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Improvement of peanut rhizobial inoculant by incorporation of plant growth promoting rhizobacteria (PGPR) as biocontrol against the seed borne fungus, Aspergillus niger

Cited by 68 publications

References 58 publications

Bacterial Compatibility in Combined Inoculations Enhances the Growth of Potato Seedlings

Bacterial Compatibility in Combined Inoculations Enhances the Growth of Potato Seedlings

Antagonistic activity of endo‐β‐1,3‐glucanase from a novel isolate, Streptomyces sp. 9X166, against black rot in orchids

Introduction: Soils and Their Promotion of Plant Growth

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